Thesis (M.S.)--University of Michigan, 1958.

Thesis (M.S.)--University of Michigan, 1974.

Thesis (M.S.)--University of Michigan, 1958.

Thesis (M.S.)--University of Michigan, 1974.

Thesis (doctoral)--Karolinska Institutet, Stockholm, 1989. / Extra t.p. with thesis statement inserted. Includes bibliographical references.

Thesis--Karolinska Institute. / Includes bibliographical references.

Male ring doves ( Streptopelia risoria ) held on long days perform more nest-building than birds held on short days. This difference persists even if circulating androgen is maintained at similar levels in both groups by castration and treatment with exogenous testosterone. It therefore appears that daylength can alter nest-building through some means other than changes in gonadal androgen production. The experiments described in this thesis examined three mechanisms which might be responsible for the effect of daylength on male nest-building. The first series of experiments tested the hypothesis that nest-building activity is greater under long days as a result of increased aromatization of testosterone into estrogen. Castrated male doves were implanted with estradiol benzoate, testosterone propionate (TP), or a combination of TP and an aromatase inhibitor, ATD, and exposed to either long or short photoperiods. Males held under long days continued to build more actively, regardless of the type of hormone being used. The results thus indicate that differences in the rate of aromatization cannot account for the effect of photoperiod. In a second series of experiments an attempt was made to determine whether an increase in the production of hypothalamic luteinizing hormone releasing hormone (LHRH) under long daylengths stimulates nest-building, as a result of a synergism with testosterone. Long- and short-day castrated males were treated with exogenous TP or TP and LHRH to see if the influence of short daylengths could be reversed by LHRH. LHRH treatment had no effect on the nesting activity of either short- or long-day birds. Finally, several tests were performed which focussed on the role of the pineal gland. Removal of the pineal caused a decrease in nesting activity in long-day birds, but not in short-day birds. The effect of pinealectomy does not depend on the presence of the gonads, since the birds displayed similar responses following castration and testosterone treatment. In the second test, the ability, of pinealectomy to nullify the stimulatory influence of long daylengths was confirmed. In addition, injections of chicken pineal extract increased nest-building in pinealectomized and in short-day pineal-intact birds, suggesting that the pineal produces a substance which is capable of stimulating nesting activity. The identity of this substance is unknown. Pilot tests using melatonin failed to show any stimulatory effect of this compound on nesting behaviour. However, serotonin, which is also present in the pineal in high concentration, did increase nest-building in short-day pinealectomized males. These results indicate that in response to long daylengths the pineal gland of male doves releases a compound, possibly serotonin, which stimulates nest-building activity. / Science, Faculty of / Zoology, Department of / Graduate

Prepartum, multiparous ewes were randomly assigned to experimental groups and sacrificed at 50(n=5), SO(n=4),115(n=5), and 140(n=4) days of gestation. Serum harvested the week prior to slaughter was assayed for progesterone (PG), prolactin (PRL) and growth hormone (GH) concentrations. Mammary tissue obtained at slaughter was assayed for receptor concentrations of progesterone (PGr), prolactin (PRLr) and insulin (Ir). Quantitative biochemical, histological and autoradiographical analyses were used to measure mammary gland growth and indicated no significant glandular growth occurs prior to 8O days of gestation. However, a major phase of parenchymal growth occurred between 8O and 115 days which coincided with significant increases in PG, PGr and PRLr concentrations. Parenchymal growth continued further into late pregnancy. GH and Ir concentrations did not change significantly during pregnancy and were not strongly correlated to growth measurements. These results suggest that mammary gland growth is receptor-mediated and direct or indirect regulation of PGr and PRLr is primarily responsible for the observed growth phenomenon. / Master of Science

published_or_final_version / Physiology / Doctoral / Doctor of Philosophy

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