Accurate methods for glottal feature extraction include the use of high-speed video imaging (HSVI). There have been previous attempts to extract these features with the acoustic recording. However, none of these methods compare their results with an objective method, such as HSVI. This thesis tests these acoustic methods against a large diverse population of 46 subjects. Two previously studied acoustic methods, as well as one introduced in this thesis, were compared against two video methods, area and displacement for open quotient (OQ) estimation. The area comparison proved to be somewhat ambiguous and challenging due to thresholding effects. The displacement comparison, which is based on glottal edge tracking, proved to be a more robust comparison method than the area. The first acoustic methods OQ estimate had a relatively small average error of 8.90% and the second method had a relatively large average error of -59.05% compared to the displacement OQ. The newly proposed method had a relatively small error of -13.75% when compared to the displacements OQ. There was some success even though there was relatively high error with the acoustic methods, however, they may be utilized to augment the features collected by HSVI for a more accurate glottal feature estimation.