In recent years, endocrine disruption of corticosteroid signaling pathways in wildlife and humans by environmental chemicals have attracted increasing attention. The integrated potential of chemicals in the aquatic environment that disrupt corticosteroid actions have been evaluated using in vitro glucocorticoid receptor (GR) mediated bioassays. Exogenous natural and synthetic corticosteroids (CSs), which are widely used in human and animal therapeutic applications, were demonstrated to be the most important GR agonists, that can potentially cause adverse effects, especially on aquatic organisms. To date, only a few studies have investigated the occurrence and behavior of GR agonists in the aquatic environment and their removal in conventional wastewater treatment plants. Furthermore, there are hardly any data reported on the removal of GR agonists by advanced water and wastewater treatment, especially those synthetic CSs with high potency. To further understand the fate of GR agonists in water and wastewater treatment processes, a sensitive and robust LC-MS/MS method was successfully developed for analyzing a wide range of GR agonists in various environmental waters. The occurrence of GR agonists in surface water and groundwater was monitored along the Lower Santa Cruz River (SCR). Several GR agonists were detected, and a trend of degradation was observed downstream the two WWTP outfalls for both surface water and groundwater. The fate of GR agonists in a local wastewater treatment plant (WWTP) was investigated, and up to 14 GR agonists were detected at different stages. Highly potent synthetic CSs, including clobetasol propionate (CBP), fluticasone propionate (FTP), fluocinolone acetonide (FCA), and triamcinolone acetonide (TCA), were poorly removed in WWTP. Negative removal of some CSs was observed in primary treatment, which may due to the deconjugation of CS conjugates. Removal of GR agonists in secondary effluent during various advanced water treatment processes, including UV, ozonation, MF, RO and chlorination, were studied. UV and RO appeared to be the most efficient treatment process for the attenuation of GR agonists, followed by ozone, while chlorination had little effects on GR agonists in water. Bench-scale experiments were then carried out to investigate the removal of GR agonists by ultraviolet based advanced oxidation processes (UV/AOPs), and powder activated carbon (PAC). UV/chlorine and UV/H2O2 were demonstrated to be effective in removal GR agonists in wastewater, and UV photolysis would be the predominant mechanism in UV/AOP processes. Four types of PACs were tested for removing GR agonists in wastewater effluent, and Cabot HDB carbon was suggested, while Calgon PWA carbon was not recommended due to its low removal efficiency.