M. Tech. Agriculture / South African unimproved indigenous goats are disease tolerant, able to survive on harsh conditions such as extreme temperatures and poor vegetation. It is therefore, important to include this breed in breeding objectives during this time of climate change and when animals are resistant to antibiotics. However, these goats are under threat of extinction and very little information on their reproductive status is recognized. In order to improve or maintain South African unimproved indigenous goats, basic science concerning male fertility, semen quality and production should be investigated to conserve genetic materials for future breeding. Conflicting results have been reported concerning the effect of semen collection techniques on buck sperm quality. Longevity of sperm following semen collection is a major limitation to use fresh semen for artificial insemination in rural communities where majority of South African unimproved indigenous bucks are kept. Extenders such as Tris, egg yolk and cow skimmed milk have been used to prolong the survivability of buck sperm in other countries, but contradictory results have been reported concerning which extender is more suitable for buck semen. Egg yolk based extenders have been used mostly in buck semen compared to Tris or cow skimmed milk based extenders. The purpose of the study was to compare semen collection techniques on South African unimproved indigenous goat semen, and the utilization of indigenous chicken egg yolk for preserving South African indigenous buck semen.

MSCAGR (Animal Science) / Department of Animal Science

MSCAGR / Department of Animal Science

Thesis (MSc. (Animal Production)) -- University of Limpopo, 2018 / A study was done to determine the effect of Acacia (A.) karroo, A. tortilis or A. nilotica leaf meal inclusion in a diet on methane emission and productivity of yearling male Boer goats fed a basal diet of Avena sativa hay. The first part of the study was conducted to determine and compare nutrient contents and tannin levels of A. karroo, A. tortilis and A. nilotica leaf meals and Avena sativa hay in a completely randomised design. The diets means were compared using Fisher’s least significant difference at the 5 % level of probability. The three Acacia species had higher (P<0.05) crude protein contents compared to Avena sativa hay, ranging from 12.42 to 14.49 %. No tannins were detected in Avena sativa hay. Among Acacia species, A. nilotica leaves had higher (P<0.05) total phenolic and total tannin contents than A. karroo and A. tortilis leaves, the latter being similar (P>0.05). All three Acacia species had similar (P>0.05) contents of condensed tannins, ranging from 1.49 to 1.76 %. However, only A. nilotica leaves had hydrolysable tannins of 128 mg/g. The second part of the study was conducted to determine the effect of A. karroo, A. tortilis or A. nilotica leaf meal inclusion level on diet intake and digestibility, methane emission, productivity and blood profiles of yearling male Boer goats. A total of three experiments were conducted and different goats were used in each experiment. A grand total of thirty six (twelve per experiment) yearling male Boer goats with initial mean live weights of 23 ± 2 kg (experiment 1), 23 ± 2 kg (experiment 2) and 23 ± 2 kg (experiment 3) were used in 21-day experiments. The goats were randomly assigned to four dietary treatments, each containing A. karroo, A. tortilis or A. nilotica leaf meal inclusion levels at 10, 15, 20 and 30 % with Avena sativa hay as a basal diet. Each goat was housed in well-ventilated individual metabolic pen. The diets were replicated three times. The data collected were subjected to analysis of covariance and analysis of variance in a completely randomized design using SAS. Differences were separated at 5 % level of probability. Additionally, regression equations were used to determine the relationships and responses in optimal diet intake and digestibility, methane emission, productivity and blood profiles of yearling male Boer goats to differing inclusion levels of A. karroo, A. tortilis and A. nilotica leaf meal. v Experiment 1 involved feeding different levels of A. karroo leaf meal inclusion to yearling male Boer goats fed Avena sativa hay as a basal diet. Results of methane emission before and during the experiment indicated that inclusion of A. karroo leaf meal in the diets of goats reduced (P<0.05) methane emission. Similarly, some of the blood parameters before and after experiment were affected (P<0.05) by inclusion of A. karroo leaf meal. However, A. karroo leaf meal inclusion level had no effect (P>0.05) on diet intake, methane emission, live weight changes and digestibility, except (P<0.05) for digestibility of crude fibre. Feed conversion ratio improved linearly with increased A. karroo leaf meal inclusion level. In the case of experiment 2, results of methane emission before and during the experiment indicated that inclusion of A. tortilis leaf meal in the diets of goats reduced (P<0.05) methane emission. Similarly, some of the blood parameters before and after the experiment were affected (P<0.05) by inclusion of A. tortilis leaf meal. However, variation in A. tortilis leaf meal inclusion level had comparable effect (P>0.05) on methane emission, live weight changes and digestibility, except (P<0.05) for digestibility of crude protein and acid detergent fibre. Diet intake and feed conversion ratio were affected (P<0.05) by A. tortilis leaf meal inclusion level. A positive relationship was observed between A. tortilis leaf meal inclusion level and diet intake. In experiment 3, results of methane emission before and during the experiment indicated that inclusion of A. nilotica leaf meal in the diets of goats reduced (P<0.05) methane emission. Similarly, some of the blood parameters before and the after experiment were affected (P<0.05) by inclusion of A. nilotica leaf meal. Apart from diet fat digestibility and feed conversion ratio, no statistical differences (P>0.05) were observed on diet intake, digestibility and live weight changes of goats fed A. nilotica leaf meal inclusion level. Positive relationships were observed between A. nilotica leaf meal inclusion level, diet fat digestibility and feed conversion ratio in male Boer goats. The high crude protein and low tannin contents in A. karroo, A. tortilis and A. nilotica leaves indicate that these Acacia species can be safe to use as a source of protein in animal nutrition if used sparingly. It was noted that nutrient intake, digestibility, vi feed conversion ratio and blood profiles of Boer goats in the present study were optimised at different inclusion levels for all the three Acacia species. This may imply that inclusion levels of A. karroo, A. tortilis and A. nilotica leaf meals for optimal productivity will depend on the Acacia species used and, also, on particular productivity parameters in question. Although, A. karroo, A. tortilis and A. nilotica leaf meal reduced methane emission, optimal reduction in methane emission was not determined. Further validation is required to determine Acacia inclusion levels for optimal methane production and emission by goats. / NRF (National Research Foundation), AGRISETA (Agricultural Sector and Education Training Authority) and NSFAS (National Students Financial Aid Scheme)

Thesis ( M. Tech. (Agriculture )) - Central University of Technology, Free State, 2014

MSCAGR (Animal Science) / Department of Animal Science / The Boer goat (Capra hircus) is one of the most desirable goat breeds for meat production. The impact of cryopreservation on the viability of its semen depends on the extenders, freezing and thawing methods. This study evaluated the effects on sperm viability in Boer goat semen extended using Bioxcell, Biladyl and Ham’s F10, and frozen in semen straws placed on a rack at 4, 5, 6 or 7 cm above the surface of liquid nitrogen. After storage in liquid nitrogen for 7 days, the frozen semen was thawed at 37 oC for 30 seconds or 90 oC for 5 seconds. Samples of sperm were also frozen to -196 oC in a programmable freezer, as the control regime for the freezing treatments. Sperm morphology, motility and viability were evaluated using the computer aided sperm analysis (CASA) system in a randomised design in which the treatments were in a 3 (extender) X 5 (freezing regime) and X 2 (thawing regime) factorial arrangement. The extenders Bioxcell and Biladyl were affected in the total motility, progressive motility and static (P<0.01), the motility was overall maintained only in straws placed at 5 cm above the liquid nitrogen level, with significant difference for the interaction extender X freezing regime in the total motility (p<0.01), non-progressive motility (p<0.05) and progressive motility (p<0.01), the 37 oC for 30 sec thawing regime had significantly more (P<0.05) in cut-head spermatozoa. Ham’s F10 extender had significantly lower normal spermatozoa (P<0.05) compare to Biladyl and Bioxcell extenders. In conclusion, the extender type, freezing and thawing regime were important factors for consideration in goat semen / NRF