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Improved Fabrication and Quality Control of Substrate Integrated Microelectrode ArraysZim, Bret E. 05 1900 (has links)
Spontaneously active monolayer neuronal networks cultured on photoetched multimicroelectrode plates (MMEPs) offer great potential for use in studying neuronal networks. However, there are many problems associated with frequent, long-term use of MMEPs. The major problems include (1) polysiloxane insulation deterioration and breakdown, (2) and loss of gold at the gold electroplated indium-tin oxide (ITO) electrodes. The objective of this investigation was to correct these major problems. Quality control measures were employed to monitor MMEP fabrication variables. The phenotypes of polysiloxane degradation were identified and classified. Factors that were found to contribute most to insulation deterioration were (1) moisture contamination during MMEP insulation, (2) loss of the quartz barrier layer from excessive exposure to basic solutions, and (3) repetitive use in culture. As a result, the insulation equipment and methods were modified to control moisture-dependent insulation deterioration, and the KOH reprocessing solution was replaced with tetramethylguanidine to prevent damage to the quartz. The problems associated with gold electroplating were solved via the addition of a pulsed-DC application of gold in a new citrate buffered electroplating solution.
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Výroba optických zrcadel pro vysokofrekvenční antény / Manufacture of optical mirrors for high-frequency antennaMuzikant, Dušan January 2011 (has links)
This thesis deals with problems with manufacturing of high frequency elliptic and hyperbolic optic mirrors, which makes one of the most important functional parts of parabolic satellites made for biggest and highest placed international astronomical radio-telescope in the world - ALMA, which is built on Chajnantor plateau in Chille in South America. Individual chapters are designed in the flow of technological process of manufacturing, containing theoretical and practical analysis of the problems. Content of the thesis leads especially to description and clarification of individual causes of problems during manufacturing.
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