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The generation of macrophages requires the coordinated responses to stimulatory and inhibitory signals that cell receive from their environment. While the up-regulation of macrophage production and survival is essential to fortify the immune system, their down-regulation is also vital to prevent macrophage related diseases and malignancy. Previous studies in goldfish showed that primary kidney macrophages release endogenous growth inducing factors into proliferative phase supernatant, which up-regulate their proliferation and survival. In this thesis, the effect of senescence phase supernatant (collected from goldfish primary kidney macrophage cultures) on goldfish primary kidney macrophages proliferation, survival and its impact on the ability of macrophages to tolerate H2O2 was analyzed. The results showed that the senescence phase supernatant down-regulated the proliferation and survival, and decreased the chemical tolerance of the cells. This indicated that the goldfish primary kidney macrophages promote targeted control of their proliferation and survival by secreting endogenous growth inhibitory factors in the senescence phase supernatant. / Physiology, Cell and Development Biology