by Ng, Shuet Fai. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1990. / Bibliography: leaves 118-137. / ACKNOWLEDGEMENTS --- p.i / ABSTRACT --- p.ii / CONTENTS --- p.iv / Chapter CHAPTER 1: --- GENERAL INTRODUCTION / Chapter I. --- The History of Gossypol --- p..4 / Chapter II. --- The Chemistry of Gossypol --- p..7 / Chapter A. --- Enantiomers of Gossypol --- p.10 / Chapter B. --- Analysis of Gossypol --- p.11 / Chapter III. --- Metabolism and Distribution of Gossypol in Body --- p.12 / Chapter IV. --- The Effect of Gossypol --- p.14 / Chapter A. --- Effect of Gossypol on Different Animal Species --- p.14 / Chapter B. --- Effect of Gossypol on Male Reproductive Organs --- p.16 / Chapter 1. --- Testis and Epididymis --- p.16 / Chapter 2. --- Spermatozoa and Spermatogenic Cells --- p.18 / Chapter C. --- Effect of Gossypol on Reproductive Hormones --- p.20 / Chapter D. --- Effect of Gossypol on Enzymes --- p.22 / Chapter 1. --- Enzymes in Capacitation --- p.23 / Chapter 2. --- The Metabolic Enzymes --- p.24 / Chapter a. --- On Glycolysis --- p.24 / Chapter b. --- On TCA Cycle --- p.26 / Chapter c. --- On Oxidative Phosphorylation --- p.26 / Chapter 3. --- Adenyl Cyclase --- p.26 / Chapter 4. --- Na+/K+-ATPase --- p.28 / Chapter E. --- Other Effects of Gossypol --- p.29 / Chapter V. --- Toxicity of Gossypol --- p.30 / Chapter A. --- General Toxic Effect --- p.30 / Chapter B. --- The Mutagenicity --- p.32 / Chapter VI. --- Clinical Trials of Gossypol --- p.33 / Chapter VII. --- The Purpose of This Study --- p.35 / Chapter A. --- Gossypol and LDH-X --- p.35 / Chapter B. --- Effect of Gossypol on Antioxidant Defense Systems / Chapter C. --- The Influence of Gossypol on Zinc Metabolism --- p.37 / Chapter CHAPTER 2: --- EFFECT OF GOSSYPOL ON LDH-X OF COCK AND DRAKE / Chapter I. --- Introduction --- p.39 / Chapter A. --- General Description of Lactate Dehydrogenase --- p.39 / Chapter B. --- Discovery of LDH-X --- p.40 / Chapter C. --- Properties of LDH-X --- p.41 / Chapter D. --- Role of LDH-X in the Spermatozoa --- p.42 / Chapter E. --- Gossypol and LDH-X --- p.43 / Chapter II. --- Materials and Methods --- p.44 / Chapter A. --- Reagents --- p.45 / Chapter B. --- Methods --- p.45 / Chapter 1. --- The Effect of Gossypol on the Spermatozoa of Cock and Drake --- p.45 / Chapter 2. --- "Measurement of LDH-X Activities in Cock, Drake, Mouse and Rat" --- p.46 / Chapter a. --- Preparation of Samples --- p.46 / Chapter b. --- Enzyme Assay --- p.47 / Chapter III. --- Results / Chapter A. --- Effect of Gossypol on the Spermatozoa of Cock and Drake --- p.48 / Chapter 1. --- Cock --- p.48 / Chapter 2. --- Drake --- p.49 / Chapter B. --- "The LDH-X Activities of Cock, Drake, Rat and Mouse" --- p.51 / Chapter IV. --- Discussion --- p.54 / Chapter CHAPTER 3: --- "THE EFFECT OF GOSSYPOL ON THE METABOLISM OF ZINC IN THE TESTIS, HAIR AND RETINA OF HAMSTER AND MOUSE" / Chapter I. --- Introduction --- p.57 / Chapter A. --- The Physiological Role of Zinc in Animals --- p.57 / Chapter B. --- The Relationship Between Gossypol and Divalentions --- p.58 / Chapter II. --- Materials and Methods --- p.60 / Chapter A. --- Reagents --- p.60 / Chapter B. --- Treatment of Animals for the Zinc Content Study / Chapter 1. --- Detection of Zinc Content in Hamster Hair --- p.61 / Chapter 2. --- "Detection of Zinc Distribution in the Hair, Testis, and Retina in Hamster and Mouse" --- p.62 / Chapter C. --- Determination of Zinc and Iron --- p.63 / Chapter III. --- Results --- p.63 / Chapter A. --- Amount of Zinc in Hamster's Hair --- p.63 / Chapter B. --- "The Distribution of Zinc Content in Hair, Testis and Retina of Hamster and Mouse" --- p.69 / Chapter IV. --- Discussion --- p.71 / Chapter CHAPTER 4: --- EFFECT OF GOSSYPOL ON THE ANTIOXIDANT DEFENSE SYSTEM IN THE TESTIS OF HAMSTER / Chapter I. --- Introduction --- p.73 / Chapter A. --- Oxygen Radicals in Living Cells --- p.75 / Chapter B. --- Oxygen Damage of Spermatozoa --- p.76 / Chapter C. --- Antioxidant Defense System --- p.77 / Chapter 1. --- Enzymatic Antioxidants --- p.78 / Chapter 2. --- Non-Enzymatic Antioxidants --- p.78 / Chapter II. --- Materials and Methods --- p.81 / Chapter A. --- Reagents --- p.81 / Chapter B. --- Experimental Animals --- p.82 / Chapter C. --- The Effect of Scavengers on the Antifertility of Gossypol --- p.83 / Chapter 1. --- Vitamin C --- p.83 / Chapter 2. --- Selenium --- p.84 / Chapter 3. --- Vitamin E --- p.85 / Chapter D. --- Chemical Interaction of Gossypol with Vitamin C and Sodium Selenite --- p.85 / Chapter E. --- Malonaldehyde Assay --- p.85 / Chapter F. --- The Effect of Gossypol on the Antioxidant Defense Enzymes --- p.86 / Chapter 1. --- Drug Treatment --- p.86 / Chapter 2. --- Preparation of Samples for Enzyme Determination --- p.87 / Chapter 3. --- Enzyme Assays --- p.87 / Chapter a. --- Se-GSH-Px --- p.88 / Chapter b. --- Glutathione-S-Transf erase --- p.88 / Chapter c. --- Catalase --- p.89 / Chapter d. --- Superoxide Dismutase --- p.90 / Chapter III. --- Results --- p.90 / Chapter A. --- The Effect of Scavengers on the Antifertility of Gossypol --- p.90 / Chapter B. --- Chemical Interaction of Gossypol with Vitamin C and sodium selenite --- p.91 / Chapter C. --- The MDA Concentration of the Testis of Hamster after Gossypol and Scavengers Injection --- p.101 / Chapter D. --- The Antioxidant Defense Enzymes --- p.101 / Chapter IV. --- Discussion --- p.109 / Chapter CHAPTER 5: --- CONCLUSION AND GENERAL DISCUSSION --- p.113 / REFERENCES --- p.118 / Chapter APPENDIX I --- THE INFLUENCE OF DIETARY LARD ON THE ANT I FERTILITY EFFECT OF GOSSYPOL --- p.138 / Chapter APPENDIX II --- PROTOCOL FOR GOSSYPOL DETERMINATION --- p.158