Nearly 80 % of child oncological patients survive treatment nowadays and live in adulthood. Therefore, one of the main current children's oncology task is not only to cure the patient but to assure the life after treatment in as highest quality as possible. Related to the quality of life is the adequate adult body height which is in these children after hematooncological treatment ussualy known as impaired. The aim of this diploma thesis was to specify the frequency and level of impaired final body height in children after hematopoietic stem cell transplantation, and find its relation to sex, diagnosis and age at the time of transplantation. Thesis criteria met 89 children (35 female), who recieved the hematopoietic stem cell transplantation between years 1989 and 2012 in University Hospital in Motol, Prague. Children were observed prospectively in Laboratory of Clinical Anthropology of the Pediatric Clinic of University Hospital in Motol since the date of their transplantation till they reach their final body height. Each survey consists of the measurement of body height and weight, BMI calculation, Tanner pubertal stages and bone age assessment by the TW3 method. These, completed by clinical data about diagnosis, growth hormone deficiency treatment, and menarche in girls, were compared to the...

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To study the effect of NaCl stress on gene expression in alfalfa (Medicago sativa L.), greenhouse and laboratory experiments were conducted with 22 cultivars during germination and post-germination growth. The ability of alfalfa cultivars to germinate at the different NaCl concentrations was not related to their postgermination performance (salt tolerance) under those conditions. Genetic effects were evident for Na and Cl ion uptake and accumulation in alfalfa shoots and roots. The strategies of alfalfa cultivars to cope with NaCl stress includes exclusion of Na from shoots and Cl from roots or from the whole plant depending on cultivar and NaCl level. The reduction in shoot dry weight was not related to water stress or reduction in Ca, Mg, or chlorophyll concentrations. Results of this study indicated that the reduction in K ion uptake and more directly the toxic effects of high Na and Cl in plant tissues contributed to the reduction in shoot dry weight . The possible subpartitioning of Na and Cl ions between different tissues, cell types, cell components, and/or the different changes in protein structure and enzyme activity at the high Na and Cl concentrations, independent of total concentration of ions, might also contribute to differences in salt tolerance (shoot dry weight) among cultivars. The factors determining shoot dry weight in alfalfa grown under the different NaCl treatments of this study were not the same for all cultivars. Sodium chloride stress induced qualitative and quantitative changes in shoot and root proteins of alfalfa. These changes were dependent on cultivar and salt concentration, as well as length of exposure to salt stress.

Submitted by JÚLIO HEBER SILVA (julioheber@yahoo.com.br) on 2018-05-16T17:24:42Z No. of bitstreams: 2 Dissertação - Cássio do Prado Borges - 2018.pdf: 1666721 bytes, checksum: f51abeeb5f0d89d62697065a578e27e8 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2018-05-17T13:23:13Z (GMT) No. of bitstreams: 2 Dissertação - Cássio do Prado Borges - 2018.pdf: 1666721 bytes, checksum: f51abeeb5f0d89d62697065a578e27e8 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Made available in DSpace on 2018-05-17T13:23:13Z (GMT). No. of bitstreams: 2 Dissertação - Cássio do Prado Borges - 2018.pdf: 1666721 bytes, checksum: f51abeeb5f0d89d62697065a578e27e8 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2018-03-29 / Fundação de Amparo à Pesquisa do Estado de Goiás - FAPEG / The objective of this work was to evaluate the influence of the application of fungicide and biostimulant, on different stages, sources and forms off aplication of nitrogen fertilization, on the agronomic characters of maize. Three experiments were carried out in the field, one for the evaluation of the application of biostimulant and fungicide, one for the evaluation of the application of only biostimulant and one for the evaluation of nitrogen fertilization in coverage. All experiments were implanted in the no - tillage system, with the same cultural treatments, in a randomized block design, with three replications. In the first experiment, it was evaluated the influence of the application of biostimulant and fungicide on the agronomic characters of maize hybrid cultivated in the first and second harvests, agricultural year 2016/2017. The other experiments were carried out only in the second harvest. The second experiment was evaluated only the effect of the application of biostimulant based on algae extract, applied in the treatment of seeds and in the phenological stages V4 and VT. The third experiment evaluated the effect of the application of nitrogen fertilization in cover, in the V4 stage, being the treatments formed by the combination of five sources of urea and two forms of application (on the surface or incorporated to the soil). The application of fungicide and biostimulant in the first harvest was only significant for stem diameter. In second crop presented for all the characters, except mass of a thousand grains. The application of biostimulant based on seaweed extract, in the treatment of seeds and in the V4 and VT stages, promoted increases in the agronomic characteristics, except for a thousand grain mass. The sources and forms of application of nitrogen fertilization based on urea did not differ from the fertilization done only in the planting for none of the characters evaluated, except for a thousand grain mass. / Objetivou se com o presente trabalho avaliar a influência da aplicação conjugada ou não de fungicida e bioestimulante em diferentes estádios fenológicos, formas e fontes de adubação nitrogenada aplicada em cobertura, sobre os caracteres agronômicos de milho. Para isso foram conduzidos três experimentos em campo, sendo um para avaliação da aplicação conjugada ou não de bioestimulante e fungicida, um para a avaliação da aplicação de somente bioestimulante e um para a avaliação de adubação nitrogenada em cobertura. Todos os experimentos foram implantados no sistema de plantio direto, com mesmos tratos culturais, em delineamento de blocos casualizados, com três repetições. No primeiro experimento avaliou se a influência da aplicação conjugada ou não de bioestimulante e fungicida nos estádios fenológicos V4 e VT, sobre os caracteres agronômicos de hibrido de milho cultivado em primeira e segunda safra, ano agrícola 2016 / 2017. Os demais experimentos foram desenvolvidos somente em segunda safra. O segundo experimento foi avaliado somente o efeito da aplicação de bioestimulante a base de extrato de algas, aplicado no tratamento de sementes e nos estádios fenológicos V4 e VT. O terceiro experimento avaliou o efeito da aplicação de adubação nitrogenada em cobertura no estádio V4, sendo os tratamentos formados pela combinação de quatro fontes de ureia e duas formas de aplicação. A aplicação conjugada ou não de fungicida e bioestimulante em primeira safra apresentou significância somente para diâmetro de colmo. Em segunda safra apresentou para todos os caracteres, exceto massa de mil grãos. A aplicação de bioestimulante a base de extrato de alga, no tratamento de sementes e nos estádios V4 e VT, promoveu incrementos nos caracteres agronômicos, exceto massa de mil grãos. As fontes e formas de aplicação de adubação nitrogenada a base de ureia em cobertura, não diferiram da adubação realizada somente no plantio para nenhum dos caracteres avaliados, exceto massa de mil grãos.