Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2015-10-08T22:13:49Z No. of bitstreams: 1 Disserta??o Final_Fabio Ribeiro Garcia.pdf: 1235311 bytes, checksum: 99c4c15b4044b2e3d060b0f7f4d595d4 (MD5) / Made available in DSpace on 2015-10-08T22:13:49Z (GMT). No. of bitstreams: 1 Disserta??o Final_Fabio Ribeiro Garcia.pdf: 1235311 bytes, checksum: 99c4c15b4044b2e3d060b0f7f4d595d4 (MD5) Previous issue date: 2013-05-07 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / bromeliad species are native to Brazil and have great potential for use as an ornamental plant, and a relevant ecological importance. Considering the devastation of the Atlantic, currently 7.3% of its original area preserved and where about 70% are endemic bromeliad is important to establish methods of propagation and ex situ conservation in order to preserve this germplasm and avoid irreversible genetic erosion. Thus, the aim of this work was to establish protocols and micropropagation in vitro conservation of A. blancheteana and A. miniata. In the first chapter, for multiplication in vitro, we used different concentrations of BAP and for rooting tested different concentrations of auxins IAA, IBA and NAA. In the second chapter we tested two protocols, was first used in MS medium supplemented with different concentrations of auxin Picloram and 2,4-D combined with BAP and the second experiment evaluated MS medium supplemented with 2,4-D or picloram in different concentrations. In the third chapter, we investigated the effect of osmotic agents, sucrose, sorbitol and mannitol in vitro conservation A. blancheteana. For the best result micropropagation for multiplication was for medium supplemented with 4.44 ?M BA. To induce somatic embryos and regeneration of the concentration of 22.5 ?M 2,4-D was the most efficient. Independent of concentration, mannitol was more efficient for in vitro conservation of A. blancheteana for 12 months. / Aechmea blancheteana (Baker) L.B. Smith e Aechmea miniata (Beer) Baker s?o esp?cies de brom?lias nativas do Brasil que possuem grande potencial para uso como planta ornamental, al?m de relevante import?ncia ecol?gica. Considerando a grande devasta??o de Mata Atl?ntica, atualmente com 7,3% de sua ?rea original preservada, onde cerca de 70% das brom?lias s?o end?micas ? importante o estabelecimento de m?todos propaga??o e conserva??o ex situ com o objetivo de preservar esse germoplasma e evitar uma eros?o gen?tica irrevers?vel. Assim, o objetivo deste trabalho estabelecer os protocolos de micropropaga??o e conserva??o in vitro de A. blancheteana e A. miniata. No primeiro cap?tulo, para a multiplica??o in vitro, foram utilizadas diferentes concentra??es de BAP e na fase de enraizamento foram testadas diferentes concentra??es das auxinas AIA, AIB e ANA. No segundo cap?tulo foram testadas a combina??o de diferentes concentra??es das auxinas Picloram e 2,4-D e da auxina 2,4-D combinada com a citocinina BAP na indu??o de embri?es som?ticos. No terceiro cap?tulo, foi avaliado o efeito dos agentes osm?ticos, sacarose, sorbitol e manitol na conserva??o in vitro de A. blancheteana. Para a micropropaga??o o melhor resultado foi obtido em meio MS suplementado com 4,44?M de BAP. Para a indu??o e regenera??o de embri?es som?ticos, a concentra??o 22,5?M de 2,4-D foi a mais eficiente. Independente da concentra??o, o manitol foi mais eficiente para a conserva??o in vitro de A. blancheteana por 12 meses.