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A longitudinal study of the covariation of skeletal growth with reference to the mandible, the metacarpals, and standing height a thesis submitted in partial fulfillment ... [orthodontics] /Minkoff, Robert. January 1959 (has links)
Thesis (M.S.)--University of Michigan, 1959.
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Electrophoretic studies on matrix vesicles from rabbit growth plate cartilage梁志衡, Leung, Chi-hang, Wilfred. January 1996 (has links)
published_or_final_version / Biochemistry / Master / Master of Philosophy
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Phenotypic characterization of cartilage cells during endochondral ossification (an avian growth plate model).January 1990 (has links)
by Lee Kwong Man, Simon. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1990. / Bibliography: leaves 88-94. / ABSTRACT --- p.I / ACKNOWLEDGEMENTS --- p.IV / TABLE OF CONTENTS --- p.V / Chapter CHAPTER ONE - --- INTRODUCTION --- p.1 / Chapter CHAPTER TWO - --- STRUCTURE OF CARTILAGE / Chapter 2.1 --- Characteristics of Cartilage --- p.4 / Chapter 2.2 --- Types of Cartilage --- p.4 / Chapter 2.3 --- Matrix --- p.5 / Chapter 2.3.1 --- Collagen --- p.5 / Chapter 2.3.2 --- Proteoglycan --- p.7 / Chapter 2.4 --- Diffusion of Solultes in Cartilage --- p.9 / Chapter 2.5 --- Chondroytes --- p.10 / Chapter CHAPTER THREE - --- PHYSIOLOGICAL CHANGES WITHIN CARTILAGE / Chapter 3.1 --- Endochondral Ossification --- p.12 / Chapter 3.2 --- Hormone Responses on Cartilage --- p.16 / Chapter 3.3 --- Degradative enzymatic system --- p.17 / Chapter 3.3.1 --- Evidence for the Role of Enzymes in osteoarthritis --- p.18 / Chapter 3.3.2 --- Neutral Protease Acivity --- p.20 / Chapter 3.3.3 --- Neutal proteases in osteoarthritis --- p.21 / Chapter 3.3.4 --- Collagenase activity in articular cartilage --- p.22 / Chapter CHAPTER FOUR - --- METHODOLOGIES / Chapter 4.1 --- Isolation of Chick Growth Plate and Articular Chondrocytes --- p.24 / Chapter 4.2 --- Countercurrent Centrifugal Elutriation --- p.25 / Chapter 4.3 --- Size Determination of Chondrocytes --- p.26 / Chapter 4.4 --- Chondrocyte Cell Culture --- p.28 / Chapter 4.5 --- Flow Cytometry Cell Cycle Analysis of Elutriated Chondrocytes --- p.28 / Chapter 4.6 --- Thymidine Incorporation Assay on Elutriated Chondrocytes --- p.29 / Chapter 4.7 --- Sulfur Incorporation Assay on Elutriated Chondrocytes --- p.30 / Chapter 4.8 --- Hyalurondiase Assay on Elutriated Chondrocytes --- p.31 / Chapter 4.9 --- Alkaline Phosphatase Assay on Elutriated Chondrocytes --- p.32 / Chapter 4.10 --- Acid Phosphatase Assay --- p.33 / Chapter 4.10.1 --- Total Acid Phosphatase Assay on Elutriated Chondrocytes --- p.33 / Chapter 4.10.2 --- Optimal pH Determination of Phosphatase in Isolated Chondrocytes --- p.33 / Chapter 4.10.3 --- Enzyme Kinetics of Acid Phosphatase of Isolated Growth Plate and Articular Chondrocytes --- p.34 / Chapter 4.10.4 --- Tartrate Inhibition Effect on Acid Phosphatase of Growth Plate and Articular Chondrocytes --- p.35 / Chapter 4.10.5 --- Distribution of Acid Phosphatase Isoenzymes Among Chondrocytes of Different Size --- p.35 / Chapter 4.11 --- Hormonal Effects on Acid and Alkaline Phosphatase Activities in Growth Plate and Articular Chondrocytes --- p.36 / Chapter CHAPTER FIVE - --- RESULTS / Chapter 5.1 --- Morphology of the Isolated Chick Chondrocytes --- p.39 / Chapter 5.2 --- Countercurrent Centrifugal Elutriation VI --- p.39 / Chapter 5.3 --- Thymidine Incorporation Assay on Elutriated Chondrocytes --- p.44 / Chapter 5.4 --- Flow Cytometer Cell Cycle Analysis of Elutriated Chondrocytes --- p.44 / Chapter 5.5 --- Sulfate Incorporation Assay on Elutriated Chondrocytes --- p.48 / Chapter 5.6 --- Hyaluronidase Assay on Elutriated Chondrocytes on --- p.48 / Chapter 5.7 --- Alkaline Phosphatase Assay on Elutriaed Chondrocytes --- p.48 / Chapter 5.8 --- Acid Phosphatase Assay --- p.52 / Chapter 5.8.1 --- pH Curve of Phosphatase in Isolated Chondrocytes --- p.52 / Chapter 5.8.2 --- Enzyme Kinetics of Acid Phosphatase oflsolated Growth Plate and Articular Chondrocytes --- p.52 / Chapter 5.8.3 --- Tartrate Inhibition Effect on Acid Phosphatae of Growth Plate and Articular Chondrocytes --- p.55 / Chapter 5.8.4 --- Distribution of Acid Phosphatase Isoenzymes Among Chondrocytes in Different Size --- p.57 / Chapter 5.9 --- Hormonal Effects on Acid and Alkaline Phosphatase Activities in Growth Plate and Articular Chondrocytes --- p.59 / Chapter CHAPTER SIX - --- DISCUSSION / Chapter 6.1 --- Identification of Chondrocyte Subpopulations --- p.63 / Chapter 6.2 --- Characterization of Chondrocyte Subpopulations --- p.72 / Chapter 6.3 --- Characterization of Acid Phosphatase in Chick Chondrocytes --- p.74 / LIST OF FIGURES --- p.84 / LIST OF TABLES --- p.87 / REFERENCES --- p.88 / Chapter APPENDIX I --- Principle of Countercurrent Centrifugal Elutriation --- p.95 / Chapter APPENDIX II --- Principle of Flow Cytometry --- p.98 / Chapter APPENDIX III --- Reagents for Experiments --- p.103
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In vitro and in situ porcine models for the studies on phenotypic characterization of cartilage cells during endochondral ossification.January 1996 (has links)
by Lee Kwong Man, Simon. / Thesis (Ph.D.)--Chinese University of Hong Kong, 1996. / Includes bibliographical references (leaves 239-277). / ABSTRACT --- p.i / ACKNOWLEDGMENT --- p.vi / PUBLICATIONS --- p.vii / ABBREVIATIONS --- p.viii / TABLE OF CONTENT --- p.xi / Chapter CHAPTER ONE --- General Introduction --- p.1 / Chapter CHAPTER TWO --- Identification and Biochemical Characterization of Various Differentiative Growth Plate Chondrocytes by Countercurrent Centrifugal Elutriation --- p.8 / Chapter CHAPTER THREE --- Differential Expression of Glycoconjugates during Endochondral Ossification in Porcine Growth Plate --- p.50 / Chapter CHAPTER FOUR --- Intra- & Extra-Cellular Free Calcium Activities of Porcine Growth Plate Chondrocytes at Various Stages of Maturation --- p.90 / Chapter CHAPTER FIVE --- A New In Situ Model for Electrophysiological Characterization of Ionic Channels in Growth Plate Chondrocytes --- p.144 / Chapter CHAPTER SIX --- Effects of Quinolones on Growth Plate Chondrocytes --- p.201 / Chapter CHAPTER SEVEN --- Summary and Conclusion --- p.226 / BIBLIOGRAPHY --- p.239
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Electrophoretic studies on matrix vesicles from rabbit growth plate cartilage /Leung, Chi-hang, Wilfred. January 1996 (has links)
Thesis (M. Phil.)--University of Hong Kong, 1996. / Includes bibliographical references (leaf 88-107).
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Essential roles of Pdia3/PLAA receptor complex and CaMKII IN 1α,25(OH)₂D₃ and Wnt5a calcium-dependent signaling pathways in osteoblasts and chondrocytesDoroudi, Maryam 08 June 2015 (has links)
The vitamin D metabolite 1,25-dihydroxyvitamin D3 [1α,25(OH)2D3] plays an important role in the regulation of musculoskeletal growth and differentiation. 1α,25(OH)2D3 mediates its effects on cells, including chondrocytes and osteoblasts, through the classical nuclear 1α,25(OH)2D3 receptor. Additionally, recent evidence indicates that several cellular responses to 1α,25(OH)2D3 are mediated via a rapid, calcium-dependent membrane-mediated pathway. These actions of 1α,25(OH)2D3 can be blocked by antibodies to protein-disulfide isomerase family A, member 3 (Pdia3), indicating that it is part of the receptor complex; however, the pathway which is activated by this receptor is not fully understood. The overall goal of this thesis was to examine the roles of phospholipase A2 activating protein (PLAA) and calcium calmodulin-dependent kinase II (CaMKII) in 1α,25(OH)2D3 rapid membrane-mediated signaling. We further investigated the interaction between two pathways regulating growth plate cartilage and endochondral bone formation, 1α,25(OH)2D3 and Wnt5a, at the receptor complex level. Results indicated that PLAA was required for mediating 1α,25(OH)2D3 signal from Pdia3. Furthermore, CaM and CaMKII were identified as mediators of 1α,25(OH)2D3-stimulated PLAA-dependent activation of cPLA2 and PKCα, and downstream biological effects. Wnt5a and 1α,25(OH)2D3 are important regulators of endochondral bone formation. This study demonstrated that 1α,25(OH)2D3 and Wnt5a mediate their effects via similar receptor components in osteoblasts and chondrocytes suggesting that these pathways may interact.
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Epiphyseal plate repair using fat interposition to reverse physeal deformity : an experimental study /Foster, Bruce Kristian. January 1989 (has links) (PDF)
Thesis (Ph. D.)--University of Adelaide, Dept. of Pathology, 1989. / Includes bibliographical references (leaves 169-197).
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Ossification of the mammalian metatarsal proliferation and differentiation in the presence/absence of a defined growth plate /Reno, Philip Louis. January 2006 (has links)
Thesis (Ph.D.)--Kent State University, 2006. / Title from PDF t.p. (viewed Mar. 5, 2009). Advisor: C. Owen Lovejoy. Keywords: growth plate, bone, epiphyses, proliferation, reserve zone, endochondral ossification, evolution, chondrocyte, histology, mouse, alligator, differential growth, PTHrP, PTH/PTHrP-receptor, Patched, Indian hedgehog, Bag-1. Includes bibliographical references (p. 144-155).
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Estrogen action in growth plate cartilageRafi, Ali January 2011 (has links)
No description available.
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Analysis of physiological death in equine chondrocytes /Ahmed, Yasser Abdel Galil. January 2007 (has links)
Thesis (Ph.D.)--University of Melbourne, School of Veterinary Science, 2007. / Typescript. Includes bibliographical references (leaves 153-176).
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