Hybrid renewable energy systems (HRESs) have become increasingly popular, especially for isolated regions. This thesis describes the design of a HRES for the isolated Ha'apai Island group in Tonga following a devastating cyclone which happened in 2014. Several renewable power generation and storage possibilities were investigated; solar, wind and battery were found to be feasible for Ha'apai. The conceptual design of a new energy storage system, the Subterranean Ocean Energy Storage System (SOESS), is also discussed as a possible alternative to batteries and a more viable substitute for an ocean renewable energy storage (ORES) system. For the proposed Ha'apai system, the optimum system configuration (solar 450 kW, wind 550 kW, battery 1,216 kAh/4,864 kW) with 90% renewable penetration was obtained using the HOMER software. Based on the optimum system configuration, load flow simulations of both the previous system and the proposed HRES were performed in DIgSILENT PowerFactory. The results of the load flow analysis show that all the transformers and transmission lines in both systems operate safely in both peak and nominal load conditions, and that the voltage levels of all LV buses are within the acceptable range of ±5%. The detailed system topology of the proposed HRES is discussed from the system implementation point of view. A unique set point control algorithm for the start-up/shut-down of the diesel generators was developed. The system dynamic performance was simulated according to the control logic during the three main switching events in DIgSILENT PowerFactory. The dynamic simulation results indicate that the proposed system would operate safely with acceptable voltage and frequency oscillations. This thesis could be used as a template for the design of other isolated HRESs with high renewable penetrations.

Hybrid renewable energy systems (HRESs) have become increasingly popular, especially for isolated regions. This thesis describes the design of a HRES for the isolated Ha'apai Island group in Tonga following a devastating cyclone which happened in 2014. Several renewable power generation and storage possibilities were investigated; solar, wind and battery were found to be feasible for Ha'apai. The conceptual design of a new energy storage system, the Subterranean Ocean Energy Storage System (SOESS), is also discussed as a possible alternative to batteries and a more viable substitute for an ocean renewable energy storage (ORES) system. For the proposed Ha'apai system, the optimum system configuration (solar 450 kW, wind 550 kW, battery 1,216 kAh/4,864 kW) with 90% renewable penetration was obtained using the HOMER software. Based on the optimum system configuration, load flow simulations of both the previous system and the proposed HRES were performed in DIgSILENT PowerFactory. The results of the load flow analysis show that all the transformers and transmission lines in both systems operate safely in both peak and nominal load conditions, and that the voltage levels of all LV buses are within the acceptable range of ±5%. The detailed system topology of the proposed HRES is discussed from the system implementation point of view. A unique set point control algorithm for the start-up/shut-down of the diesel generators was developed. The system dynamic performance was simulated according to the control logic during the three main switching events in DIgSILENT PowerFactory. The dynamic simulation results indicate that the proposed system would operate safely with acceptable voltage and frequency oscillations. This thesis could be used as a template for the design of other isolated HRESs with high renewable penetrations.

Systemic Lupus Erythematosus (SLE) is a complex autoimmune disease. HTLV-1 Related Endogenous Sequence (HRES-1), a human endogenous retrovirus, produces 2 retroviral-like Gag capsid proteins (p8 and p15) that share significant sequence homology to the U1-subunit of the small ribonucleoprotein complex (U1sn-RNP), an autoantigen of lupus. The central hypothesis is that molecular mimicry between HRES-1 and U1sn-RNP serves as a priming event in SLE via the production of cross-reactive autoantibodies. Anti-HRES-1/U1sn-RNP serological responses in subjects with SLE and comparison populations were characterized. An overlapping peptide set mapping the HRES-1 p8 and p15 proteins was used. SLE subjects produce IgG to several regions of HRES-1. Healthy subjects or those with RA, HIV-1 infection, or HTLV-1-infection produced no significant anti-HRES-1 IgG. Anti-HRES-1 antibodies deposited in the kidneys of patients with SLE glomerulonephritis were identified. Our data suggests that HRES-1 plays a role in SLE by means of a molecular mimicry mechanism with U1sn-RNP.

Yes / The cost of electricity produced by thermal power plants in Republic of Djibouti is relatively high at about $0.32/ kWh. This is due to its dependence on imported oil coupled with fluctuating oil prices. Consequently, the customer pays a high electricity bill. However, Djibouti is endowed with indigenous renewable energy resources such as a good solar irradiance of 5.92 kWh/ m2 day, a potential geothermal energy estimated up to 1000 MW, and few sites with annual wind speed higher than 6 m/s. The goal of this paper is, therefore, to assess an economic evaluation of different grid connected hybrid renewable energy systems to a residential urban house located in Tadjourah city (11.7913◦ N, 42.8796◦ E) in the North-Eastern part of Djibouti to reduce the cost of electricity from the grid. To reach this objective, a powerful software tool called HOMER (Hybrid Optimization Model for Electric Renewables) has been used to find the optimum hybrid energy system using real wind and solar irradiation data. The results obtained from this study show that the best economical suited combination of hybrid renewable energy system is a PV-Wind grid connected system. This study shows also that potentially the indigenous renewable energy contribution, in Tadjourah, can be as much as 77 % with 47 % of solar and 30% of Wind energy. The Net Present Cost, the Levelized Cost of Energy, and the operating cost of the optimal HRES are $337, $0.002/kWh and $1,025/year, respectively. When compared with the average cost of grid-only connection of $0.32/kWh, the optimal hybrid renewable energy system is more economical and will save 51 % of the cost that the customer must pay when using only the electricity from the grid.

This thesis presents a parallel-series two-bridge DC/DC converter topology with the ability to operate with ZVS over a wide input and load range. The intended application is power conditioning systems (PCS) of photovoltaic (PV) arrays used in hybrid renewable energy system architectures. The proposed topology provides two degrees of freedom which allows the PV-PCS to regulate the DC-link voltage, while tracking the maximum power point (MPP) of the PV array. This topology distributes the main power into two bridges and the phase-shift between the two bridges and provides another degree of freedom for the PCS to track the MPP. The proposed topology is also able to achieve soft-switching over a wide range. The power conditioning system shows a modular structure to efficiently transfer the power to the load as the main power is divided between two bridges. In addition, the proposed control scheme provides complete decoupling between the input side controller from the output side controller in order to perform MPPT and regulate the the DC-link voltage simultaneously. A 2kW Experimental prototype has been provided to validate the feasibility and performance of the converter. Experimental results prove that the converter is able to regulate the DC-link voltage and track the maximum power extracted from the PV array simultaneously. / Thesis (Master, Electrical & Computer Engineering) -- Queen's University, 2012-04-18 19:51:43.405

Hormone-dependent cancers (HDCs), such as those of the prostate, ovary, breast and endometrium, share characteristics that indicate similar underlying mechanisms of carcinogenesis. Through steroid hormone signalling on "down-stream" target genes, the growth, development and progression of HDCs are regulated. One such family of target genes, highly expressed in HDCs and regulated by steroid hormones, are the tissue kallikreins (KLKs). The KLKs are a multigene family of serine proteases involved in physiological processes such as blood pressure regulation, inflammation, and tumour development and progression via the hydrolysis of specific substrates. Although the KLK gene family is clearly implicated in tumourigenesis, the precise roles played by these genes are largely unknown. Additionally, except for the androgen-responsive genes, KLK2 and KLK3, the mechanisms underlying their hormonal regulation in HDCs are yet to be identified. The initial focus of this thesis was to examine the regulation of the kallikreins, KLK1 and KLK4, by estradiol and progesterone in endometrial and breast cancer cell lines. From these studies, progesterone clearly regulated KLK4 expression in T47D cells and therefore, the focus of the remaining studies was to further examine this regulation at the transcriptional level. An overview of the results obtained is detailed below. Human K1 and hK4 protein levels were increased by 10 nmol/L estradiol benzoate, progesterone, or a combination of the two, over 48 hours in the endometrial cancer cell line, KLE. However, these same treatments resulted in no change in KLK1 gene or hK1 protein levels in the endometrial cancer cell lines, HEC1A or HEC1B (only hK1 analysed). Progesterone treatment (0-100 nmol/L) over 24 hours resulted in a clear increase in KLK4 mRNA at the 10 nmol/L dose in the breast cancer cell line, T47D. Additionally, treatment of T47D cells with 10 nmol/L progesterone over 0-48 hr, resulted in the rapid expression of the hK4 protein at 2 hr which was sustained for 24 hr. Further analysis of this latter progesterone regulation with the antiprogesterone, RU486, over 24 hours, resulted in an observable decrease in hK4 levels at 1 µmol/L RU486. Although the estrogen and progesterone regulation of the hK1 protein was not further analysed, the data obtained for hK4 regulation in T47D cell lines, supported the premise that this gene was progesterone-responsive. The rapid expression of hK4 protein by progesterone at two hours suggests that KLK4 transcription is directly coupled to progesterone regulation, perhaps through progesterone receptor (PR) binding to progesterone-responsive regions within the KLK4 promoter or far "up-stream" regions. Thus, the following further studies were performed. To test this hypothesis, the transcription initiation site (TIS) and 5' flanking regions of the KLK4 gene in T47D cells were interrogated. Primer extension and 5' RACE identified the TIS 78 bp 5' of the putative ATG site for translation as identified by Korkmaz et al. (2001). This KLK4 gene transcript consists of only four exons, and thus excludes the pre/pro signal peptide. Although a TATA-box is not present within -25 to -30 bp 5' of the identified TIS, a number of consensus binding motifs for Sp1 and estrogen receptor half-sites were identified. It is possible that the Sp1 sites are involved in the basal levels of transcription for this gene. Additionally, a putative progesterone response element (PRE) was identified in the far "up-stream" regions of the KLK4 gene. Basal levels of transcription were observed within the KLK4 proximal promoter region when coupled to a luciferase reporter gene and transfected into T47D cell lines. Additionally, the KLK4 proximal promoter region did not induce the luciferase reporter gene expression when progesterone was added to the system, however, estradiol was inhibitory for luciferase gene expression. This suggests that the proximal promoter region of the KLK4 gene could contain functional EREs but not PREs. In keeping with this hypothesis, some ER half-sites were identified, but PR sites were not obvious within this region. The identified PRE in the far "up-stream" region of the KLK4 gene assembled the progesterone receptor in vitro, and in vivo, as assessed by electromobility shift assays and chromatin immunoprecipitation assays (EMSAs and ChIPs), respectively. The binding of the PR to the KLK4 PRE was successfully competed out by a PR antibody and not by an androgen receptor antibody, and thus confirms the specificity of the KLK4 PRE-PR complex. Additionally, the PR was recruited and assembled onto and off the progesterone-responsive KLK4 region in a cyclic fashion. Thus, these data strongly suggest that the PR represents one of the core components of a transcription complex for the KLK4 gene, and presumably also contributes to the expression of this gene. Moreover, these data suggest a functional coordination between the PR and the KLK4 progesterone-responsive region in T47D cells, and thus, provide a model system to further study these events in vivo.