The human use of landscape causes fragmentation and loss of original habitats. Different species vary in their sensitivity to habitat loss. Especially carnivores can be more sensitive to decrease of their habitat because of lower abundance of their prey. On the other hand several opportunistic carnivores can profit in human modified habitats. This study was carried out in the České Budějovice basin, Czech Republic during the years 2008 and 2009 and the aim of this study was to determine carnivore{\crq}s habitat preferences in the fragmented landscape. During these two years were seven carnivore species monitored in forest patches by using scent stations. Records of this monitoring were compared with physiognomy of these patches and structure of surrounding landscape.