Source: Dissertation Abstracts International, Volume: 32-11, Section: B, page: 6486. / Thesis (Ph.D.)--The Florida State University, 1972.

Source: Dissertation Abstracts International, Volume: 35-07, Section: B, page: 3406. / Thesis (Ph.D.)--The Florida State University, 1974.

Source: Dissertation Abstracts International, Volume: 38-09, Section: B, page: 4149. / Thesis (Ph.D.)--The Florida State University, 1977.

Source: Dissertation Abstracts International, Volume: 30-12, Section: B, page: 5566. / Thesis (Ph.D.)--The Florida State University, 1969.

No description available.

Thesis (Ph. D.)--University OF MICHIGAN.

The aim of this study was to compare the neuromotor development of preterm and full-term infants over the first year of life. Thirty-six preterm infants born at less than 37 weeks gestation and who were neurologically normal at 12 months of age were evaluated on a variety of neuromotor assessments at term, and three, six, nine and twelve months adjusted age. The results were compared to a cross-sectional group of fifteen full-term infants, evaluated on the same reflex and motor assessments. Preterm infants performed significantly more poorly than the full-term infants, with particular lags noted at term and at three and twelve months of age. No significant differences were found between groups of preterm infants born at (i) 24-26, (ii) 27-29, and (iii) $ geq$30 weeks gestation. The differences between the preterm and full-term infants were attributed to factors associated with preterm birth and the evaluation procedures.

Needless to say, it is imperative for workers in the neurological field to know and understand the circulatory system of the Central Nervous System. The basal vessels of the brain, especially the circle of Willis and basilar artery are of particular interest and importance as they are the main and only source of metabolism to such vital structures as basal ganglia, hypothalamus, midbrain and pons. Many investigators have therefore contributed to the knowledge of the course, tributary area, variations, anomalies etc., from the anatomical, physiological and pathological aspects.

Although tissue human growth hormone receptors (GHRs) have been identified from as early as the first trimester of fetal life, little is known about their functional role. We recently reported that fetal hGHRs appear to be relatively immature or have adapted to the in utero environment [194]. To further characterize these age-related differences, we have examined certain aspects of the hGHR structure as well as its signalling pathways. Initial cDNA sequencing and immunoblotting analyses showed no difference in hGHR molecular size. We then used RT-PCR to investigate levels of an alternatively spliced mRNA for a truncated (1--279) hGHR (T) which acts as a dominant negative to the full-length (FL) receptor; however, fetal hepatocytes showed T/FL ratios similar to postnatal liver (0.07 +/- 0.02 vs. 0.09 +/- 0.01, M +/- SEM). Western blotting was used to assess developmental differences in the relative abundance of downstream signalling factors. Stimulatory Jak and Stat proteins were detected in all fetal hepatocytes and postnatal livers tested (13 weeks fetal age to 62 years). (Abstract shortened by UMI.)

The vertebrate body is patterned asymmetrically along the left-right axis. Left-right patterning is required to establish correct asymmetric organ formation and positioning, and ultimately it is essential for normal physiological functioning. Misregulation in this process can lead to severe physiological defects in multiple organs, including the heart and gut. Pitx2c is a paired-like homeodomain transcription factor and is crucial for translating left-right signals into asymmetric morphogenesis. It is asymmetrically expressed in the left lateral plate mesoderm and continues to be expressed on the left side of organ primordia that are destined to become asymmetrically formed or positioned organs. Previous work studying Pitx2c has demonstrated its evolutionarily conserved role in left-right patterning. While the homeodomain is important for Pitx2c's function, our lab identified a putative interaction domain in the N-terminus of Pitx2c that is required for its function in left-right patterning. To further characterize the role of Pitx2c, I used a yeast two-hybrid screen to identify candidate protein interaction partners of its N-terminus. Thirty-two candidates were identified from the screen. Candidates that were isolated at least twice were prioritized for further analysis. After eliminating non-coding clones, false positive interactions, and confirming interactions of the candidate putative interaction domains with the Pitx2c N-terminus in yeast, six candidates remained: Anti-silencing function 1 homolog B (S. cerevisiae; Asf1b), Eukaryotic translation initiation factor 3, subunit A (Eif3a), Eukaryotic translation initiation factor 3, subunit M (Eif3m), Niemann-Pick C type 2 (Npc2), Serine/arginine-rich splicing factor 2 (Srsf2), and Serine/arginine-rich splicing factor 15 (Srsf15). mRNA Expression patterns of these six Pitx2c candidate protein interaction partners was analyzed by whole mount in situ hybridization and compared to Pitx2c expression patterns. All candidates were expressed symmetrically in the embryo, including tissues where Pitx2c is asymmetrically expressed. In the chick embryo, Npc2 and Srsf2 were expressed in a tissue-specific manner from HH stage 10 to HH stage 26, while Eif3a and Eif3m were broadly expressed throughout the embryo from HH stage 10 and only became enriched in particular tissues at HH stage 24 to HH stage 26. All candidates were coexpressed with Pitx2c in the looped heart tube. Only Srsf2 was coexpressed with Pitx2c in the lateral plate mesoderm. The known functions of these candidates are consistent with the known role of Pitx2c in transcriptional regulation and suggests new roles for Pitx2c in mRNA processing and translational regulation during left-right patterning. / Le corps des vertébrés est structuré asymétriquement le long de l'axe gauche-droit. La latéralité est requise afin d'établir la formation des organes ainsi que leur positionnement qui, ultimement, sont essentielles pour un fonctionnement physiologique normal des vertébrés. Lors de ce processus, une mauvaise régulation peut causer des troubles physiologiques sévères dans plusieurs organes incluant le cœur et l'intestin.Le facteur de transcription homéodomaine Pitx2c joue un rôle essentiel lors de la traduction des signaux gauche-droit en morphogénèse asymétrique des organes. Pitx2c est exprimé de façon asymétrique dans le mésoderme de la plaque latérale gauche et continu d'être exprimé du côté gauche des futurs organes asymétriques. Quelques études ont démontré que Pitx2c a un rôle qui est conservé lors de l'évolution dans la latéralité. Le rôle de l'homéodomaine est important pour la fonction de Pitx2c et nous avons démontré dans notre laboratoire qu'un domaine d'interaction de la partie N-terminale de Pitx2c est aussi important pour sa fonction lors de la latéralité.Afin de caractériser d'avantage le rôle de Pitx2c, j'ai utilisé la méthode de double hybride afin d'identifier des protéines candidates d'interaction avec la partie N-terminale de Pitx2c. Trente-deux candidats ont été sélectionnés. De ce nombre, les faux positifs ainsi que les candidats qui représentent région la non-codante d'une protéine ont été supprimés ce qui a permis de réduire la liste à six candidats : Anti-silencing function 1 homolog B (S. cerevisiae; Asf1b), Eukaryotic translation initiation factor 3, subunit A (Eif3a), Eukaryotic translation initiation factor 3, subunit M (Eif3m), Niemann-Pick C type 2 (Npc2), Serine/arginine-rich splicing factor 2 (Srsf2), ainsi que Serine/arginine-rich splicing factor 15 (Srsf15).Les patrons d'expression des six protéines candidates potentielles d'interaction avec Pitx2c ont été examinés par hybridation in situ et ont été comparé avec le patron d'expression de Pitx2c. Chez tous les candidats, l'expression asymétrique dans les embryons de poulet a été observée incluant dans les tissus où Pitx2c est aussi exprimé asymétriquement. Npc2 et Srsf2 sont exprimés de façon spécifique dans certains tissus à partir du stade HH10 chez le poulet. Eif3a et Eif3m sont largement exprimés dans les embryons à partir du stade HH10 mais leur expression est enrichie dans certains tissus à partir du stade HH22. L'expression de tous les candidats coïncide avec celle de Pitx2c dans la courbure du tube cardiaque. Seulement l'expression de Srsf2 coïncide avec celle de Pitx2c dans le mésoderme de la plaque latérale gauche. Les fonctions des différents candidats suggèrent que ces partenaires potentiels d'interactions de la partie N-terminale de Pitx2c pourraient jouer un rôle important lors de la régulation de la transcription, la formation de l'ARN messager et la régulation de la traduction lors de la latéralité.