Made available in DSpace on 2016-05-02T13:55:17Z (GMT). No. of bitstreams: 1 Luiz Silva Souza-dissertacao.pdf: 3490471 bytes, checksum: c67d9a32f1552af2738fbcd53645873e (MD5) Previous issue date: 2012-07-10 / In addition to being an important source of nutrients, the potential therapeutic effectiveness of H. annuus L. seeds has been previously demonstrated; the beneficial properties of H. annuus L. seeds include an ability to relieve asthmatic symptoms, protective effects on the stomach, benefits to healing, and anti-inflammatory and anti-microbial properties. This research evaluated the mutagenicity and anti-mutagenicity of oil and tincture of H. annuus L. seeds using the micronucleus assay in bone marrow of mice. The interaction between these preparations and the genotoxic effects of doxorubicin (DOX) was also analyzed. Experimental groups were evaluated after 24-48 h of treatment with N-Nitroso-N-ethylurea (NEU) and DOX (positive controls), NaCl (a negative control), a sunflower tincture (250-2,000 mg.Kg-1 of THALS) and two sources of sunflower oils (250-2,000 mg.Kg-1 of POHALS and FOHALS). Anti-mutagenic assays were carried out using the sunflower tincture and oils separately and in combination with these controls. The frequency of micronucleated polychromatic erythrocytes (MNPCEs) was significantly different (p &#61500; 0.05) between (i) the positive and negative control treatments, (ii) the positive controls and animals treated with THALS and (iii) animals treated with THALS and and THALS combined with the positive controls. However, a slight genotoxicity was observed in the animals treated with the combination of THALS+DOX. Both sources of oils (FOHALS and POHALS) revealed similar results; in these groups, the frequencies of MNPCEs were similar to those observed in negative controls. Statistically significant differences were also observed between the sunflower oil treatments and their associated positive controls. There was no genotoxicity (clastogenicity/aneugenicity) observed in THALS, POHALS and FOHALS regardless of the dose, time (except FOHALS) and gender of mouse (except POHALS and FOHALS). The moderate anti-genotoxic effects of THALS suggest a potential slight protective mechanism against DOX-induced genotoxic effects. / A efetividade terapêutica potencial das sementes de H. annuus L. tem sido demonstrada (alívio dos sintomas da asma, efeitos da proteção gástrica, cicatrização, propriedades anti-inflamatórias e antimicrobianas), em adição à sua importante fonte de nutrientes. Esta pesquisa avaliou a mutagenicidade e anti-mutagenicidade do óleo e da tintura de sementes de H. annuus L. usando o ensaio do micronúcleo em medula óssea de camundongos. A associação sobre os efeitos genotóxicos induzidos pela doxorrubicina (DOX) também foi analisada. Grupos experimentais foram avaliados após 24-48h de tratamento com N-Nitroso-N-ethylurea (NUE) e DOX (controles positivos), NaCl (controle negativo), tintura (250-2.000 mg.Kg-1 de THALS) e duas fontes de óleo de girassol (250-2.000 mg.Kg-1 de POHALS e FOHALS). Ensaios anti-mutagênicos foram realizados usando os controles positives associados com a tintura e óleos de girassol, separadamente. A frequência de eritrócitos policromáticos micronucleados (MNPCEs) foi estatisticamente diferente (p < 0,05) entre (i) os tratamentos controles positives e negativo, (ii) controles positivos e tratamento com THALS e (iii) tratamento com THALS e sua associação aos controles positivos. Contudo, uma leve genotoxicidade foi observada na associação THALS+DOX. Ambas as fontes de óleos (FOHALS e POHALS) revelaram resultados similares, cujas frequências de MNPCEs foram consistentes com aqueles observados no controle negativo. Diferenças significativas também ocorreram entre os tratamentos de óleos e suas associações com os controles positivos. A ausência de genotoxicidade (clastogenia/aneugenia) da THALS, POHALS e FOHALS pode ser inferida independentemente da dose, tempo (exceto para FOHALS) e gênero de camundongo (exceto para POHALS e FOHALS). Efeitos anti-genotóxicos moderados de THALS sugerem um potencial mecanismo ligeiramente protetor sobre os efeitos genotóxicos induzidos pela DOX.