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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Extended X-Ray Absorption Fine Structure and Redox Potential Studies of Heme-Substituted Horseradish Peroxidase and Myoglobin

He, Bing 01 May 1995 (has links)
Heme-substituted horseradish peroxidases and myoglobins were reconstituted from the apoenzyme using mesoheme and diacetyldeuteroheme. X-ray absorption spectroscopy was used to determine the dimensions of the active sites of these heme-substituted proteins, and were compared with those of the proto-hemeproteins. The change in the active-site structure corresponded with the electron withdrawing and donating effects of the different side chains. The oxidation-reduction potentials of Fe4+/Fe3+ couples of the heme-substituted proteins were measured at pH 7 with K2IrC16. The oxidation-reduction potential sequence for compound I/compound II was diacetyldeutero-> proto-> meso-in horseradish peroxidase. The oxidation-reduction potential sequence for compound II /ferric was meso-> proto-> diacetyldeutero-in both HRP and myoglobin. These results indicate that the oxidation of ferric to ferryl form may be related to a radical mechanism. A net charge theory was also proposed to explain these results.

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