Erratum pasted inside back end paper. / Bibliography: leaves 174-201. / xvii, 202, [77] leaves, [8] leaves of plates : ill. (chiefly col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Establishes that the herbicide carbetamide degrades rapidly upon repeated application to soil, when compared to a previously untreated soil. / Thesis (Ph.D.)--University of Adelaide, Dept. of Crop Protection, 1997

Call number: LD2668 .T4 1964 C74 / Master of Science

Nine distinctive bacteria were isolated from a diclofop-methyl-degrading-consortium biofilm and their genomic DNA extracted for genetic characterization. With one exception, all the isolates contained plasmid(s). The consortium degrades diclofopmethyl producing a number of metabolites that are intermediates or substrates for bacteria that degrade chlorinated aromatic compounds. Accordingly, specific catabolic gene PCR primers for chlorinated degradation pathways were designed and tested to determine if these genes are involved in diclofop-methyl degradation. DNA homology analysis between the PCR products and the known catabolic genes investigated by Southern hybridization analysis and by sequencing, suggested that novel catabolic genes are functioning in the isolates. Specific fluorescent oligonucleotides were designed for two of the isolates following the 16S rDNA sequencing and the identification of each of the isolates. These probes were successfully used in whole cell hybridization and will be used in subsequent studies of the microbial ecology of the consortium.

No description available.

Digitized by Kansas Correctional Industries

Soil samples were collected from several corn fields with history of atrazine (herbicide) application. Samples were inoculated into Erlenmeyer flasks each containing 50m1 of minimal basal salts medium amended with 100 ppm atrazine as sole nitrogen source. Flasks were shaken at 200 rpm at ambient temperature and were examined daily for one week for microbial growth and/or disappearance of atrazine. Promising consortia were subcultured for further additional enrichments before characterization of potentially active protein (enzyme)fractions. Proteins from cell-free and cellbound fractions were compared for ability to denature atrazine. Following gel permeation chromatography, isolated protein fractions were examined for atrazinefound in the cell-bound fractions capable degrading degradation. Two were found in the cell-free fractions (approx. Mol. wts. 55kDa and 180kDa) and one (55 kDa) atrazine to hydroxyatrazine. Atrazine and its breakdown products (hydroxyatrazine in particular) were detected via HPLC using C18 and C8 columns with absorbance at 229 nm. / Department of Biology

Typescript. / Thesis (Ph. D.)--University of Hawaii at Manoa, 1978. / Bibliography: leaves 193-205. / Microfiche. / xv, 205 leaves ill. 28 cm

The use of chemicals to control economic pests has grown in this century both in the amount and the diversity of materials used (Headley and Lewis, 1967). About ten billion pounds of pesticides have been used in the United States since 1945. It is estimated that there are 340 million acres of cultivated lands in the Continental United States. Of this amount, approximately 119 million acres were sprayed with herbicides, 97 million acres were treated with insecticides and 25 million acres were treated with fungicides and other minor pesticides (Matsumura, 1972).

Graduation date: 1999

Diss. (sammanfattning) Uppsala : Sveriges lantbruksuniversitet, 2006. / Härtill 4 uppsatser.