Environmental conditions can influence epigenetic variability of clonal organisms. These environmentally induced changes have potential to persist in following generations. In theory, this transgenerational memory could allow clonal (and especially apomictic) plants to partly compensate reduced genetic variability and could enhance adaptative ability of apomictic populations. According to Muller's ratchet model, asexual organisms amass deleterious mutations, which could lead to their extinction. Possible transgenerational memory could partly compensate for this. It could also account for longtime survivorship of asexual organisms in environment. This thesis deals with transgenerational memory of triploid apomictic populations of Hieracium alpinum, with completely missing sexuality and thus genetically uniform offspring. Transgenerational effect in Hieracium alpinum was studied on clonal lineages originated from 5 populations (Norway, Austria, Bosnia and Herzegovina and Slovakia). Seeds were planted in cultivation experiment I in 3 treatments (control, added nutrients, added salicylic acid) and vegetative and generative traits were measured. Plants responded to nutrient addition but not to salicylic acid. Treatment with nutrient addition increased biomass, number of leaves and SLA (specific leaf...

The mode of reproduction can greatly influence the demography and the evolutionary success of the taxon. In the case of autonomous asexual formation seeds are apomictic taxa fully independent of pollinators and compatible partners. For sexual taxa with strict autoincompatibility it is the opposite, i.e. sexual taxa need pollinators and compatible partners for birth of offspring. Therefore, in marginal population and for more extreme situation with lower pollinating activity will have apomictic taxa a higher level of reproductive assurance than sexual taxa vascular plants. This hypothesis was tested in natural populations of apomictic and sexual taxa. In the diploma thesis we therefore investigate the method mode of reproduction and reproductive assurance of 52 taxa of the genus Hieracium s. str. (family Asteraceae) in Europe. Of these, 12 were diploid sexually diploid taxa and 42 polyploid apomictic reproductive taxa. From these taxa we harvested seeds from fully developed capitulum and we determined the potential (total number of seeds in the capitulum) and the realized (the percentage of well-developed seeds at the capitulum). The ploidy of the offspring (the embryos and the seedling) and method origins of seeds we examined using flow cytometry. The results show that the plants of diploid species...

<div>Grand Sable Dunes, as a perched dune field on the shore of Lake Superior, is a sensitive</div><div>ecosystem subject to continual disturbance. Repeated natural disturbances necessitate specialized</div><div>plant communities to develop. There were two objectives of my research in this system that are</div><div>treated in separate chapters. They include: 1) the quantification of successional changes in the</div><div>plant community over time, and the identification of population demography changes for rare</div><div>species within the dunes and 2) the evaluation of evaluate in pollinator species for two plants</div><div>Hieracium caespitosum (Yellow Hawkweed) and Lithospermum caroliniense (Carolina</div><div>Puccoon).</div><div>For the first objective, target plant community composition and structures (i.e. richness,</div><div>diversity) were quantified in 2011 and 2018 across Grand Sable Dunes in 1 m² quadrats.</div><div>Additionally, two relatively rare plant species (Cirsium pitcheri and Tanacetum bipinnatum)</div><div>were selected to quantify demographic (i.e. flowering, non-flowering) patterns and changes over</div><div>time. Samples for C. pitcheri and T. bipinnatum were acquired via circle-plots 2.5 m in diameter.</div><div>Population comparisons between 2011 and 2018 illustrate minimal change in community</div><div>structure (richness and diversity). Composition increased slightly with eight species occurring in</div><div>2018, but not 2011. Additionally, community similarity was high (~78%) between the two years.</div><div>C. pitcheri occurrence was inversely related to presence of other species. Plant community</div><div>composition in eastern and western survey zones within the dunes appear to be diverging. Minor</div><div>changes in the plant community composition and structures indicate successional changes have</div><div>occurred, but without major disturbance. This divergence in community composition may be</div><div>related to weather related incidents associated with Lake Superior disturbance potential.</div><div>The secondary objective concerns pollinator species on two similar plant species found in</div><div>the Grand Sable Dunes. Individuals of H. caespitosum and L. caroliniense were observed and all</div><div>floral visitors were identified to family. The majority of arthropod families were observed</div><div>10</div><div>visiting both H. caespitosum and L. caroliniense, with an absence of typically important families</div><div>(e.g. Apidae, Bombiliidae). Halictidae, Muscidae and Syrphidae were the most common visitors,</div><div>with L. caroliniense attracting far more Muscidae than their H. caespitosum competitors.</div><div>Overlap in visitors for both species was observed, which may lead to decreased reproduction in</div><div>L. caroliniense.</div>

Invasive species represent a major concern; they can result in serious ecological and economic losses and are recognised as one of the most serious threats to global species diversity. Plant invasions are of particular concern in New Zealand, which has high proportions of both naturalised and endemic plant species. In this thesis I focussed on the invasive plant Hieracium lepidulum, an exotic weed introduced from Europe to New Zealand prior to 1941. It is invasive in a variety of habitats in the South Island, where it has steadily increased in distribution and abundance over the last 50 years, and is thought to have detrimental impacts on native plant communities. I investigated factors influencing its invasion success and tested for impacts on native plant communities, making extensive use of existing plots into which H. lepidulum was experimentally introduced in 2003. I examined how community richness, turnover, resource availability and propagule pressure of the invader interacted to determine the invasion success of H. lepidulum. Results differed markedly above and below treeline. Above treeline, plots with higher richness and turnover were more invaded; below treeline, plots with higher available light were more invaded. In both habitats, these findings were modified by the influence of propagule pressure; at low propagule pressure, site characteristics were non-significant in explaining invasion success, while at higher propagule pressure these effects became significant. To test for impacts resulting in altered community composition and structure, I looked for changes in community richness, diversity and evenness subsequent to H. lepidulum introduction. As impacts may be more apparent at fine spatial scales, I made measurements at a 5 x 5 cm cell scale in addition to the established 30 x 30 cm plot scale. Plot species richness increased from 2003 to 2009 and a component of this increase was associated with H. lepidulum density. Other relationships between the plant community and H. lepidulum were generally non-significant. Results showed that H. lepidulum has had no negative effects on community richness, evenness or diversity. Despite being able to opportunistically colonise grassland sites with high turnover, and forest sites subject to canopy disturbance, dependant on propagule pressure, it appears H. lepidulum has not impacted community composition or structure.