As fossil fuel supplies decrease and concerns of climate change increase, the search for alternative sources of fuel has pushed biomass crops to the forefront of discussion. Saccharum spontaneum readily hybridizes with commercial sugarcane and lends cold tolerance and greater yields to the hybrid progeny, called energycane. Twenty genotypes were tested against an energycane variety (Ho 02-113) as a control. Two locations were tested: the HH Leveck Animal Research Center (planted in 2019), and the Bearden Dairy Research Center (planted in 2020). The Bearden Dairy Research Center yielded significantly higher than the HH Leveck Animal Research Center regarding dry matter yield (P < 0.0001). Dry Matter yields were significantly greater in the plant cane year than in the ratoon year at the HH Leveck Animal Research Center (P = 0.0008). There were significant differences among replications in both locations regarding dry matter.