Typescript (photocopy). / Digitized by Kansas Correctional Industries / Department: Biology.

Includes bibliographical references (leaves 281-328) / iii, 328 leaves : ill. ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / The experiments reported are investigations of the pituitary of rats stunted by undernutrition imposed between birth and 3 weeks of age. / Thesis (Ph.D.)--University of Adelaide, Dept. of Animal Physiology, 1971

Includes bibliographical references (leaves 281-328) The experiments reported are investigations of the pituitary of rats stunted by undernutrition imposed between birth and 3 weeks of age.

Includes articles co-authored by the author during the preparation of this thesis. / Includes erratum on back leaf. / Includes bibliographical references (leaves 257-299). / xvii, 300 [27] leaves : ill. (some col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Examines regional variations in trabecular bone remodelling and bone loss following oophorectomy in the distal femur of the rat. The studies reveal a complex interaction between weight bearing and ovarian hormone deficiency, and show that physiological signals exist which can negate all adverse effects of postmenopausal osteoporosis / Thesis (Ph.D.)--Adelaide University, Dept. of Physiology, 2001

No description available.

Taste buds are spindle-shaped collections of taste receptor cells located in the surface epithelium of the oral cavity. Taste receptor cells are specialized sensory epithelial cells that are responsible for the transmission of taste information to the taste nerves. Immunocytochemical evidence of the neuropeptide cholecystokinin in various taste cells of the rat has been presented by our lab (Herness 1991). These results have prompted our investigation of the messenger RNA encoding this peptide in rat taste cells. CCK may play an important role in taste signal transmission or modulation.Three areas of the oral cavity were investigated for the presence of CCK mRNA. Two of these areas contain collections of taste buds in well-defined structures called papillae. Circumvallate papillae, located on the posterior surface of the tongue, and foliate papillae, located on the lateral surfaces of the tongue, were sectioned and probed for CCK mRNA using non-radiographic in situ hybridization. The third area investigated was the nasoincisor duct, located on the roof of the oral cavity. This duct contains isolated taste buds within the surface epithelium near the opening to the oral cavity.The results of this study confirm the presence of CCK mRNA in all three areas studied. Taste buds located in the circumvallate papillae, foliate papillae and in the nasoincisor duct all contain taste cells that express CCK mRNA. These results are verified by the absence of labeled cells in negative control experiments. The negative controls consists of the omission of probe, anti-DIG antibody, and the application of a sense probe. The immunocytochemical results show only a subset of taste cells labeled for the CCK peptide while the in situ results depict all cells in the bud labeled for CCK mRNA. The in situ results very closely parallel the immunocytochemical results previously obtained by our lab, although with in situ hybridization epithelial staining is more prominent. The surface epithelium contains the messenger RNA encoding CCK likely because taste receptor cells are derived from the lingual epithelium.Several roles for CCK can be considered in taste physiology. Taste reception and taste signal transduction is not fully understood. Also the localization and pharmacology of CCK receptors in taste systems awaits investigation. These two areas must be studied further to understand the function of CCK in taste cells. / Department of Biology

The purpose of these studies was to determine whether lactogen-dependent Nb2-11c cells and lactogen-independent Nb2-SP cells differ with respect to morphology and autocrine growth control. To this end, the ultrastructural and surface morphology of both Nb2 cell lines was analyzed and the autocrine growth modulatory activity of Nb2 cell conditioned medium (Nb2-CM) was determined. The autocrine growth inhibitory activity of Nb2-CM was biologically characterized and attempts were made to biochemically characterize and purify the Nb2 cell autocrine growth inhibitor as well as to determine its mechanism of action. Quantitative analysis of transmission electron micrographs reveals that the ultrastructural morphology of lactogen-dependent Nb2-11c cells differs from that of lactogen-independent Nb2-SP cells. Nb2-11c cells exhibit a greater incidence and volume density of nuclear pockets, whereas the incidence and volume density of lipid droplets is greater in the Nb2-SP cell line. Surface feature of Nb2-11c and Nb2-SP cells, as examined with scanning electron microscopy, and indistinguishable. Nb2-11c and Nb2-SP cells share a common mode of growth control in the form of constitutive secretion of an autocrine inhibitory factor. Medium conditioned by either Nb2-11c or Nb2-SP cells inhibits the growth of both cell lines. Nb2-CM-mediated growth inhibition is dose-dependent and reversible. Nb2-CM does not induce quiescence or cell death, but rather, causes a delay in the progression of cells through all phases of the cell cycle. Nb2 cell proliferation stimulated by a variety of mitogens is inhibited by Nb2-CM. Nb2-CM also has the ability to inhibit the growth of normal rat splenocytes as well as MCF-7 human breast cancer cells. Biochemical analysis of Nb2-CM was equivocal; however, indirect evidence suggests that the autocrine growth inhibitory factor produced by Nb2 cells may be a prostaglandin or another arachadonic acid metabolite since the growth inhibitory activity of Nb2-CM is reduced when CM is prepared in the presence of indomethacin. Interestingly, levels of prostaglandin F₁(α) are elevated in CM-treated culture supernatants. Examination of other signal transduction systems in Nb2 cells suggests that neither cAMP activation, polyamine biosynthesis, nor protein kinase C activation mediate or influence the inhibitory effect of Nb2-CM.

Zhou Liran. / "June 2003." / Thesis (Ph.D.)--Chinese University of Hong Kong, 2003. / Includes bibliographical references (p. 182-216). / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Mode of access: World Wide Web. / Abstracts in English and Chinese.

Tsang Suk Ying. / "August 2003." / Thesis (Ph.D.)--Chinese University of Hong Kong, 2003. / Includes bibliographical references (p. 196-226). / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Mode of access: World Wide Web. / Abstracts in English and Chinese.

Lau, Pui Ling. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2011. / Includes bibliographical references (leaves 84-94). / Abstracts in English and Chinese. / Abstract (English) --- p.i / Abstract (Chinese) --- p.v / Acknowledgements --- p.ix / Contents --- p.x / List of Figures --- p.xiv / Chapter Chapter 1 --- General Introduction / Chapter 1.1 --- Regulations of gametogenesis and mass spawning in corals --- p.1 / Chapter 1.1.1 --- Endogenous cues --- p.2 / Chapter 1.1.2 --- Environmental cues --- p.3 / Chapter 1.2 --- Studies on black corals --- p.5 / Chapter 1.2.1 --- Introduction of black corals --- p.5 / Chapter 1.2.2 --- Black corals harvesting --- p.6 / Chapter 1.2.3 --- Biodiversity and distribution of black corals in Chinese and Hong Kong waters --- p.8 / Chapter 1.2.4 --- Threats to black corals --- p.9 / Chapter 1.3 --- Significance and objectives of the present study --- p.11 / Chapter 1.3.1 --- Objectives --- p.12 / Chapter 1.3.2 --- "The targeted species, Antipathes curvata and the study site" --- p.13 / Chapter 1.4 --- Thesis outline --- p.14 / Chapter Chapter 2 --- "Reproductive Biology of Antipatharian Black Coral, Antipathes curvata in Lan Giio Shui, Hong Kong" / Chapter 2.1 --- Introduction --- p.18 / Chapter 2.1.1 --- Coral Reproduction --- p.20 / Chapter 2.1.2 --- Sexual reproduction in black corals --- p.21 / Chapter 2.2 --- Materials and methods --- p.24 / Chapter 2.2.1 --- Sample collections and pre-treatment --- p.24 / Chapter 2.2.2 --- Histological processing --- p.25 / Chapter 2.2.3 --- Light microscopy --- p.26 / Chapter 2.2.4 --- Gametogenesis --- p.27 / Chapter 2.2.5 --- Environmental and statistical analysis --- p.27 / Chapter 2.3 --- Results --- p.28 / Chapter 2.3.1 --- General reproductive mode --- p.28 / Chapter 2.3.2 --- "Sex ratio, size at sexual maturity and density of gamete" --- p.28 / Chapter 2.3.3 --- Characteristics of polyps and gametes of A. curvata --- p.29 / Chapter 2.3.4 --- Changes in geometric diameter of gametes overtime --- p.30 / Chapter 2.3.5 --- Developmental stages of gametogenesis --- p.31 / Chapter 2.3.5.1 --- Oogenesis --- p.31 / Chapter 2.3.5.2 --- Spermatogenesis --- p.32 / Chapter 2.3.6 --- Development of oocytes and spermaries over time --- p.34 / Chapter 2.3.7 --- Correlation of black coral reproduction with seawater temperature --- p.35 / Chapter 2.3.8 --- Gametogenesis in individual colonies --- p.36 / Chapter 2.3.8.1 --- Female colonies --- p.37 / Chapter 2.3.8.2 --- Male colonies --- p.39 / Chapter 2.4 --- Discussion --- p.40 / Chapter 2.4.1 --- Asynchronization of gametogenic cycle --- p.40 / Chapter 2.4.2 --- Possible effect of seawater temperature on reproduction of A. curvata --- p.43 / Chapter Chapter 3 --- Detection of the Sex Steroid 17β-estradiol and its Possible Roles on Gametogenesis in Black Corals Antipathes curvata from Hong Kong / Chapter 3.1 --- Introduction --- p.55 / Chapter 3.1.1 --- "Roles of sex hormone, 17(3-estradiol (E2) in the reproduction of vertebrates" --- p.58 / Chapter 3.1.2 --- Roles of vertebrate-type sex steroids in Cnidaria --- p.59 / Chapter 3.2 --- Materials and methods --- p.63 / Chapter 3.2.1 --- Study site --- p.63 / Chapter 3.2.2 --- 17β-estradiol (E2) extraction --- p.63 / Chapter 3.2.3 --- 17β-estradiol (E2) assay --- p.65 / Chapter 3.2.4 --- Calculation and assay validation --- p.65 / Chapter 3.2.5 --- Gametogenesis of A. curvata --- p.66 / Chapter 3.2.6 --- Seawater temperature and statistical analysis --- p.67 / Chapter 3.3 --- Results --- p.67 / Chapter 3.3.1 --- Seasonal profile of E2 --- p.67 / Chapter 3.3.2 --- Gametogenesis --- p.68 / Chapter 3.3.3 --- Correlation with seawater temperature --- p.69 / Chapter 3.4 --- Discussion --- p.70 / Chapter Chapter 4 --- Summary and Perspectives --- p.78 / References --- p.84