This study investigates the bioactivity of bioprinted scaffolds cultured with adult human dermal fibroblast cells (aHDF) used for tissue regeneration by employing Lumen X+ 3D bioprinter. Polyethylene glycol diacrylate (PEGDA) is the widely used bioink in these Lumen bioprinters. Although PEGDA is meritorious for its photopolymerization capabilities and biological and biomedical applications, it has very minimal inherent cell adhesion and proliferation properties. Hence in order to improvise this challenge we demonstrated the development of a novel kind of tissue-specific hybrid bioinks utilizing PEGDA that has been altered by incorporating short, self-assembling dipeptides that are based on FF or Phe-Phe hydrogels. To leverage the bioactivity of FF peptides, we first encapsulated adult human dermal fibroblasts (aHDF) in Fmoc-FF hydrogel and investigated how the HDF cells interacted to the microenvironment of the peptide in terms of adhesion and material-cell interactions. Further, we fabricated three different sets of honeycomb lumen lattice constructs: (i) Matrigel coating on lumen lattice, (ii) peptide coating on lumen lattice, and (iii) peptide infused lumen lattice lumen was printed by combing PEGDA bioinks. We investigated the potential ability of these scaffolds to enhance cell attachment rate, proliferation, and expansion of human dermal fibroblast cells and drug-delivering abilities.