This study is to explore the staffs of Kaohsiung city government for: (1) The relationships among job stress, quality of work life, humor leadership, and worksite health promotion; (2) The moderating effect of humor leadership toward job stress and quality of work life; (3) The moderating effect of worksite health promotion toward job stress and quality of work life; (4) The differences of sample characters on job stress, quality of work life, humor leadership, and worksite health promotion. The questionnaire survey was conducted in the study. Based on the data from Department of Budget, Accounting and Statistics of Kaohsiung city government, the budgeted staffs in 2009 are 10,840 persons who were located into 11 institute¡¦s catagories. According to the staffs numbers, we measured by ratio sampling (8%-10%), and associated with convenient sampling. Total 1005 questionnaires were distributed to the government staffs and 71 public schools staffs, and the valid questionnaires were 800 copies with return rate of 79.6%. This study adopted exploratory factor analysis (EFA), confirmatory factor analysis (CFA), reliability analysis, descriptive statistics, independent-sample t-test, one-way ANOVA, Structural Equation Modeling (SEM), correlation analysis and multiple regression. The findings are: (1) The government staffs have more stress on outside-giving, and they were mostly satisfied with leadership style of their supervisors; (2) Extrinsic effort and Overcommitment negatively and significantly affect quality of work life; Self-esteem and job promotion positively and significantly affect quality of work life; (3) Extrinsic effort and overcommitment negatively and significantly affect the balance of job/life; Self-esteem and job promotion positively and significantly affect quality of work life; (4) Humor leadership is a moderator toward extrinsic effort, self-esteem, overcommitment and quality of work life; (5) Health life, comfortable environment, friendly worksite are partial moderators toward self-esteem, job promotion, overcommitment and job characteristics; (6) Part of personal characters have significant differences in job stress, quality of work life, humor leadership, and worksite health promotion. This study aims to the relationship between job stress and quality of work life from the staffs of Kaohsiung city government, and could confirm humor leadership and worksite health promotion to be a moderator partially affecting job stress and quality of work life. The results could be the reference to provide Kaohsiung city government and related institutes to work on the strategy for reducing job stress and increasing quality of work life.