Thesis. / Includes bibliographical references.

Thesis (Ph. D.).--Catholic University of America, 1945. / Vita.

Thesis (Ph. D.)--University of Wisconsin--Madison, 1969. / Vita. Typescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

Thesis (M.S.)--University of Wisconsin--Madison, 1969. / eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

Thesis (M.S.)--Ohio State University, 1985. / Includes bibliographical references (leaves 204-208). Available online via OhioLINK's ETD Center.

M.Sc. (Chemistry) / This study was directed towards the investigation of oxidations by dimethyldioxirane, a new and versatile oxidant. To begin, the oxidation of various simple saturated hydrocarbons was examined. These oxidations gave alcohols in yields that varied considerably. This investigation showed that dimethyldioxirane reacts chemoselectively and that a preference is shown for tertiary C-H bonds and even more so for tertiary cis C-H bonds in bicyclic six-membered ring compounds and tertiary benzylic C-H bonds. A kinetic study of the oxidations of benzylic C-H bonds of various cumene derivatives by dimethyldioxirane confirmed the electrophilic nature of this reagent. In order to investigate the chemoselectivity of oxidations by dimethyldioxirane, the oxidation of eight steroids was examined. These oxidations were very selective and rarely gave more than two products. In all but one case, the oxidations selectively produced alcohols in good yields. The preference of dimethyldioxirane for tertiary cis C-H bonds in cyclic compounds and tertiary benzylic C-H bonds was confirmed by the oxidation' of an estrone derivative and two 5,8-5teroids. In all three cases the anticipated compounds were obtained as the major products. This oxidative method for the synthesis of 5,B-hydroxy steroids may provide a novel route for the synthesis of cardioactive steroids. The oxidation of a furo[2,3-b]benzofurane derivative was investigated to conclude this study of oxidations by dimethyldioxirane. The succesful oxidation of this compound at C-3a would furnish a synthon for aflatoxin M1 in a single step. Unfortunately, no product was obtained for the oxidation of the furo[2,3-b]benzofurane derivative by dimethyldioxirane. Futher studies into the source of this lack of reactivity are currently being undertaken.

[No abstract available] / Science, Faculty of / Chemistry, Department of / Graduate

The solubility of a long chain paraffin hydrocarbon (C₂₆H₅₈) over a temperature range of 75° has been measured in acetic, propionic and ethyl butyric acids. The phase relationship for the acetic acid - hexacosane system differs considerably from that of the other two. The results show that propionic acid is the best solvent to use for recrystallization and purification of a long chain hydrocarbon. / Applied Science, Faculty of / Chemical and Biological Engineering, Department of / Graduate

A four-part investigation was described in which first, the use of thiolignin and lignosulfonate to remove a factor involved in growth limitation in hydrocarbon fermentation was investigated; secondly, the performance of the Cyelone-fermenter in hydrocarbon fermentation was evaluated; thirdly, continuous hydrocarbon fermentation with reference to hydrocarbon, nitrogen, thiolignin and dilution rate was studied; and fourthly, the mechanism of n-decane oxidation was studied manometrically and gas chromatographically. The addition of polymerized lignosulfonate and thiolignin into the hydrocarbon fermentation media greatly, increased the fermentation rate and the yield of biomass. Lignin itself did not appear to be decomposed during the fermentation. Resting cell studies of Pseudomonas desmolytica S(11) indicated that the oxygen consumption increased with the decreasing n-alkane carbon number and did not parallel the production of total biomass. The greatest biomass occurred using n-undecane and decreased sharply with lower and higher n-alkanes. The individual n-alkanes in kerosene were not degraded uniformly, the lower ones were used preferentially. Thiolignin not only increased the rate of utilization of these lower n-alkanes but also extended microbial acceptability to the higher n-alkanes. Gas chromatographic analyses revealed that five monocarboxylic fatty acids corresponding to C(8), C(9), C(10, C(11), and C(12) were present in the fermentation fluid. The Cyclone-fermenter was found to be very suitable for hydrocarbon fermentation. The hydrocarbon, kerosene, was fermented with a pure bacterial culture in a continuous process for 250 hours without any noticeable change in the culture behavior. Moreover, the addition of various culture medium ingredients could be optimized to produce maximum cell yield or maximum acid production. Manometric and gas chromatographic studies revealed that cell-free extracts of 2(11) oxidized n-decane to n-decanol and n-decanoic acid, whereas the partially purified hydrocarbon-oxidizing enzyme only oxidized n-decane to decanol. The n-decane-oxidizing enzyme could be precipitated by 30% (NH4)2 SO4 and had a narrow optimal pH around 7.0. The enzyme also required a dialyzed, heat stable 60% (NH4)2 SO4 supernatant fraction and NAD for maximum enzyme activity. Ferrous, manganous and calcium ions did not stimulate the enzyme activity. It seemed that the enzymatic attack on n-decane occurred primarily at the terminal carbon atom and this was manifested by the fact that n-decanol, n-decanal and n-decanoic acid supported good growth for S(11). / Land and Food Systems, Faculty of / Graduate

The investigations to be presented in this thesis arose from studies on the respiration of mitochondria isolated from pig skeletal muscle. These animals developed the syndrome of Malignant Hypermedia after exposure to halothane - a halogenated hydrocarbon used as an inhalation anesthetic agent.