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Propriétés mécaniques et nanotribologiques de monocouches auto-assemblées de microgels de poly(NIPAM) cationique en milieux aqueux / Mechanical and lubricant properties of self-assembled layers of poly(NIPAM)-based cationic microgels in waterVialar, Pierre 22 November 2018 (has links)
Le but de cette thèse est de faire évoluer les connaissances et la compréhension des systèmes lubrifiants en milieux aqueux, synthétiques comme biologiques. Pour cela, nous élaborons des systèmes de monocouches auto-assemblées de microgels thermosensibles de pNIPAM cationiques afin d’en étudier les propriétés mécaniques et nanotribologiques. Nous mettons au point plusieurs synthèses de microgels afin d’étudier l’effet de l’élasticité sur le comportement tribologique. Nous regardons également l’effet de la nature du greffage des microgels en sur-face, en élaborant une méthode de couplage chimique novatrice, pour comparer les propriétés de monocouches physi- et chimisorbées. Nous étudions les propriétés mécaniques en mi-lieux aqueux des couches des différents microgels en fonction de la température, de la nature du greffage et du sel en présence, à l’aide d’une Microbalance à Cristal de Quartz avec mesure de Dissipation (QCM-D). Le coeur de notre étude est réalisé à l’aide d’un Appareil de Forces de Surface (SFA) modifié pour permettre des mesures tribologiques, dont les résultats seront traités en deux parties. La première consiste à caractériser les forces normales de surface lors-que l’on comprime deux surfaces décorées de microgels. La seconde est constituée de l’ana-lyse de ces surfaces sous compression et cisaillement. Nous explorons les propriétés lubrifiantes du système et observons l’apparition une force de normale dépendant de la vitesse de cisaillement, et dont nous cherchons l’origine. Nous avons ainsi découvert un mécanisme propre au substrat souple, décoré de particules discrètes avec un contact répulsif sans friction à longue portée. / The aim of this project is to advance the knowledge and understanding of lubricating systems, whether synthetic or biological, in aqueous media. For this purpose, we develop self-assem-bled monolayer 2D-arrays of cationic pNIPAM thermosensitive microgels in order to study their mechanical and nanotribological properties. We establish several synthetic routes to modulate the microgel rigidity and study its effect on the tribological behaviour. We also look at the effect of the grafting nature of microgels on the substrate, by developing an innovative chemical coupling method, to compare the properties of physisorbed and chemisorbed mon-olayers. We probe the mechanical properties of the microgel layers in aqueous environment while varying the temperature, the nature of the grafting and the salts added to the system, primarily by using a Quartz Crystal Microbalance with Dissipation monitoring (QCM-D). The core of our study is performed using a modified Surface Forces Apparatus (SFA) which allows for tribological measurements, the results of which will be treated in two parts. First, we char-acterise the normal surface forces when compressing two surfaces decorated with the micro-gel layers. Second, we study the behaviour of these surfaces under compression and shear. We explore their lubricant properties and observe the appearance of a shear-induced velocity-dependent lift force, whose origin we seek to determine. We thus discovered a mechanism specific to a compliant substrate, decorated with discrete particles presenting a repulsive con-tact without friction at long range.
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Conductive and recognitive hydrogels for biosensing applicationsBayer, Carolyn Louise 09 April 2012 (has links)
Human disease processes are often characterized by a deviation from the normal physiological concentration of critical biomarkers. The detection of disease biomarkers requires the development of novel sensing methods which are sensitive, specific, efficient and low-cost. To address this need, a novel conductive and recognitive hydrogel composite material has been developed. This work investigated the fabrication methods, the chemical and physical composition, the sensing capabilities, and the biocompatibility of the proposed conductive and recognitive hydrogel composite materials. The conductive polymer was found to respond by changing conductivity in the presence of biomolecules. Specificity can then be incorporated into the system by integrating the conductive polymer with a molecularly imprinted hydrogel. The demonstration of a conductive and recognitive hydrogel composite is a step towards the integration of these materials into close-loop sensing and drug delivery systems. / text
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Protein hydrogels as tissue engineering scaffoldsHaji Ruslan, Khairunnisa Nabilah January 2015 (has links)
Hydrogels aim to mimic the natural living environment by entrapping large amount of water or biological fluids in their polymeric network. There has been growing interest in the development of peptide and protein hydrogels, due to their improved biocompatibility, biodegradability and biological properties in comparison to purely synthetic polymer hydrogels. Under the appropriate conditions, biomacromolecular protein hydrogels can self-assemble into ordered meso- to macroscopic supramolecules with better resulting networks that promote tissue development. The work presented here mainly focuses on producing protein hydrogels with controlled physical properties useful for tissue regeneration process and drug delivery applications. Hen egg white lysozyme (HEWL) hydrogels were studied in the presence of water and different reducing agents forming three HEWL systems including HEWL/water, HEWL/DTT and HEWL/TCEP gels. Strong, self-supporting HEWL gels were successfully prepared in the range of pH 2 to 7, using a temperature of 85°C. At pH 2, the protein denaturation in water was relatively slow resulting in a high percentage of turn structure (~50%) that promotes HEWL gelation after 3 days of heating. No lysozyme gelation in water was observed at pH 3, 4 and 7 even after 21 days of heating. A small quantity of DTT (~20 mM) was added to encourage lysozyme unfolding and HEWL/DTT samples formed gels at higher pH including at physiological pH. The pH 2 HEWL/water gel was found to be stronger but more brittle than pH 7 HEWL/DTT gel. It was observed there were some irregularities in the distribution of pH 2 fibrils (~7µm in length) that form large pore sizes within the network. The pH 7 sample contained shorter and stiff fibrils with repetitive polygon-shaped mesh network. The use of TCEP, which is a stronger reductant than DTT, led to the formation of self-supporting HEWL gels between pH 3.5 and 5.5. The highest storage modulus was observed at pH 5, which is related to the high β-sheet content of the sample (~45%). In addition, a promising strategy has been devised to form thermoresponsive HEWL hydrogels by synthesising and incorporating a small fraction of lysozyme-PNIPAAm bioconjugates into the major protein matrix. Results show the thermoresponsive nature of PNIPAAm was conferred to HEWL protein that exhibits higher storage stability in response to changing temperature.
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Small Diameter Vascular Substitues Based on Physical Chitosan Hydrogels : Proof of Concept / Substitut vasculaire de petit calibre à base d’hydrogels physiques de chitosane : preuve de conceptMalaise, Sébastien 09 April 2014 (has links)
Le chitosane présente des propriétés biologiques (biocompatibilité, biorésorbabilité, bioactivité) idéalement adaptées à des applications en ingénierie tissulaire. Dans cette étude partenariale (Programme ANR TECSAN 2010 ChitoArt), nous avons travaillé à l'élaboration d'hydrogels physiques de chitosane à propriétés physico-chimiques et biologiques variées et contrôlées, sans utilisation d'agents de réticulation externes. Ces hydrogels sont envisagés sous forme de tube mono ou pluri-membranaires pour une utilisation en tant que substituts vasculaires de petit diamètre (<6mm). En effet, l'ingénierie vasculaire présente, encore de nos jours, de nombreuses limitations lorsqu'il est question de vaisseaux de petits calibres. Notre démarche consiste en la modulation des paramètres structuraux (degré d'acétylation, masse molaire) et environnementaux (concentration du bain de gélification, du collodion) intervenants dans le procédé d'élaboration des hydrogels pour atteindre les critères physiques, biologiques et mécaniques compatibles avec cette application. L'étude morphologique des hydrogels par Cryo-Microscopie Électronique à Balayage (Cryo-MEB), via une méthode de préparation originale a permis une meilleure compréhension de l'organisation micro-structurale et multi-échelle des hydrogels de chitosane. Cette approche fondamentale a été couplée à une évaluation in vivo des propriétés biologiques des hydrogels ainsi qu'a des caractérisations mécaniques des substituts vasculaires. En particulier, l'évaluation de la suturabilité de nos substituts a mené au développement d'une formulation donnant lieu à des hydrogels physiques de chitosane suturables ayant fait l'objet d'un dépôt de brevet (N° de dépôt FR1363099). Le contrôle et la modulation des paramètres d'élaboration des hydrogels ont permis l'obtention de substituts vasculaire cellularisables et respectant les exigences (suture, compliance, résistance à l'éclatement) concernant leur implantation in vivo / Chitosan presents biological properties (biocompatibility, bioresorbability, bioactivity) ideally suited for tissue engineering. In this partnership study (ANR TECSAN 2010 ChitoArt program), we worked at the elaboration of physical chitosan hydrogels presenting various and controlled physicochemical and biological properties, without any external crosslinkers. These hydrogels are envisioned under mono- or poly-membranous tubes for small diameter vascular substitutes (<6mm) purposes. Indeed, vascular engineering presents, even today, numerous limitations for small calibre vessels. Our strategy consists in the modulation of both structural (degree of acetylation, molar mass) and environmental (neutralization bath and collodion composition and concentration) parameters involved in hydrogels elaboration process in order to reach physical, biological and mechanical requirements suitable for this application. The study of hydrogels morphology by Cryo-Scanning Electron Microscopy (Cryo-SEM), using an original sample preparation method led to a better comprehension of chitosan hydrogels fine structure and multi-scale organization. This fundamental approach was conducted through the in vivo biological evaluation of hydrogels but also to mechanical characterizations of vascular substitutes. In particular, our substitutes were evaluated in term of suture retention resulting in the development of a formulation that led to suturable physical chitosan hydrogels, which were protected by a patent (Deposit number: FR1363099). Hydrogels elaboration parameters control and modulation have resulted in the development of colonisable vascular substitutes matching their in vivo implantation requirements (suture retention, compliance, burst pressure)
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Development and validation of a microfluidic hydrogel platform for osteochondral tissue engineeringGoldman, Stephen M. 07 January 2016 (has links)
Due to the inability of intra-articular injuries to adequately self-heal, current therapies are largely focused on palliative care and restoration of joint function rather than true regeneration. Subsequently tissue engineering of chondral and osteochondral tissue constructs has emerged as a promising strategy for the repair of partial and full-thickness intra-articular defects. Unfortunately, the fabrication of large tissue constructs is plagued by poor nutrient transport to the interior of the tissue resulting in poor tissue growth and necrosis. Further, for the specific case of osteochondral grafts, the presence of two distinct tissue types offers additional challenges related to cell sourcing, scaffolding strategies, and bioprocessing. To overcome these constraints, this dissertation was focused on the development and validation of a microfluidic hydrogel platform which reduces nutrient transport limitations within an engineered tissue construct through a serpentine microfluidic network embedded within the developing tissue. To this end, a microfluidic hydrogel was designed to meet the nutrition requirements of a developing tissue and validated through the cultivation of chondral tissue constructs of clinically relevant thicknesses. Additionally, optimal bioprocessing conditions with respect to morphogen delivery and hydrodynamic loading were pursued for the production of bony and cartilaginous tissue from bone marrow derived mesenchymal stem cells. Finally, the optimal bioprocessing conditions were implemented within MSC laden microfluidic hydrogels to spatially engineer the matrix composition of a biphasic osteochondral graft through directed differentiation.
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Occlusion of the left atrial appendage using catheter-delivered hydrogels for prevention of thromboembolic phenomenaZimbroff, Andrew David 15 October 2014 (has links)
The Left Atrial Appendage, once thought to be "a relatively insignificant portion of cardiac anatomy," has currently been realized to possess "important pathological associations [1a]" particularly in its role in promoting serious, frequent thromboembolic events common in individuals suffering from Atrial Fibrillation. Prior approaches to mitigating these events have either required invasive procedures, proved less than fully effective, or presented with problematic sequelae of their own. This work will present a new procedure that addresses both the prevention of the thromboembolic events and the correction of the shortcomings of the major prior methods utilized. A compliant hydrogel that can conform to the geometry of the appendage is proposed as a more effective method of occluding the chamber. This material would be transported to the LAA in liquid form via a multi-lumen catheter, and then solidify within the chamber to form a solid plug. Previous research has identified a candidate hydrogel, comprised of PEG-tetra-thiol and Dextran vinyl sulfone as a candidate hydrogel for this application. Experimental work has investigated fluid properties of the material, as well as degradation and swelling properties of the material. Results from this experimentation were used for fluid transport analysis, and for evaluation of anchoring force of the hydrogel within the chamber. Finally, subfunctions of the occlusion procedure were modeled and tested. During the actual procedure, a catheter balloon will isolate the appendage from the rest of the heart. A model was developed to study interactions between the appendage and this balloon. Additionally, due to fast solidification time, hydrogel components in the surgical procedure will be mixed in a mixing chamber at the tip of the catheter. Potential mixing chamber designs were modeled, and a ternary diffusion model was developed to better understand hydrogel mixing. Prototypes for both these subfunctions were built and tested as well. Additional analysis looked at the overall occlusion procedure, and how various subfunctions interacted with each other. / text
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The parallel synthesis of a new class of glycodendrimersMcWatt, Martin Joseph January 2000 (has links)
No description available.
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Development of a Thermosensitive Trimethyl Chitosan Hydrogel for in situ Tissue EngineeringUnknown Date (has links)
Chitosan was widely studied for applications in tissue regeneration, because of its biodegradability and biocompatibility. However, its insolubility in a neutral solution and long gelation time limit its wide application in tissue engineering. In this thesis, a new chitosan-based biomaterial was synthesized, and its chemical structure and solubility were characterized. Afterwards, the gelation properties (crosslinker, crosslink time, swelling ratio, drug release and biocompatibility) of TMC material was investigated. Results show that TMC has higher water solubility than chitosan. The TMC liquid solution can transform to a hydrogel quickly at body temperature. The formed hydrogel controlled the release of the model protein. Cytotoxicity result shows the cationic TMC hydrogel brings a toxic effect on stromal cells but it may have the potential to inhibit bacteria or cancer cells, although more studied are required to confirm its potential functions. In summary, this new TMC hydrogel has a promising potential in biomedical fields. / Includes bibliography. / Thesis (M.S.)--Florida Atlantic University, 2018. / FAU Electronic Theses and Dissertations Collection
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Polyoxazoline derivatives for the design of polymer brushes and hydrogelsTang, Pei January 2018 (has links)
Hydrophilic POx have very similar behaviour with PEG owing to its peptdomimetic structure, however, POx shows higher chemical stability than PEG and can be further functionalised via substitute R in the side chains or at the end of the chain. In addition, PEtOx has been approved as an indirect food additive by FDA which may indicate the possibility of good immunogenicity of POx-based materials. Synthetic surfaces with reproducibility and biocompatibility for in vitro cell culture offer lots of advantages on adherent cells. A variety of synthetic polymers as well as properties like mechanical and chemical robustness resulting polymer brushes prior to other surface modification methods. Synthetic hydrogels can be further modified and allow a variety of mechanical and biochemical properties that determine the cell phenotype which makes it a good candidate for biomedical applications. Our work has focused on the design of polyoxazolines with controlled end chains for the design of such hydrogels and polymer brushes. In the second chapter, we review the synthesis of defined polyoxazoline and its applications, synthesis of non-fouling surfaces, fabricated hydrogels and characterisations. In the third chapter, we explore the design of poly(2-oxazolines) with controlled end chains and characterise the structure and control of initiation and termination steps. A range of initiators (bromide, iodide as well as tosylates) and termination agents were used to introduce functionalisable or polymerisable end groups on poly(2-oxazolines). Microwave assisted synthesis was used for polyoxazoline synthesis. Polyoxazolines can be simply synthesized in relatively mild conditions using this approach. The structure of the resulting polymers is characterised by NMR, MALDI-ToF and FTIR. In the third chapter, we explore the use of polymerisable polyoxazolines for the design of grafted from polymer brushes. The growth of poly(oxazoline) brushes was studied first and the resulting polymer brushes characterised. We then explored the functionalization of polymer brushes using thiol-ene chemistry and their protein resistance for cell and protein patterning. Hence, we explored the design of polyoxazoline nonfouling coatings. These surfaces allow the control of surface properties such as protein adsorption and bio-functionalization. In the fourth chapter, we designed a series of thiolated poly(oxazolines) to be used for the design of hydrogels crosslinked via thiol-ene chemistry. We fully characterised the thiolated polymers designed and studied the formation of hydrogels using an alkene-functionalised polyoxazoline and a range of thiolated crosslinkers with polyethylene glycol and poly(oxazoline) backbones. Synthetic hydrogels have attracted much attention recently for in vitro cell culture as they allow the control of the properties of soft biomaterials (mechanics, cell adhesion, degradation). Importantly, the gelation conditions used for 3D cell encapsulation are essential as they allow controlling the mechanics and stability of the gel, whilst curing in mild, non-toxic conditions. Properties such as hydrogel chemistry, macroscopic and nanoscale mechanical properties and degradation have indeed been shown to strongly affect cell phenotype and the use of these materials for tissue engineering. To study gelation in situ, photo-rheology was used to characterise the properties and kinetics of the resulting hydrogels. Here, we investigated the formation of hydrogels with different multi-arm PEG thiols. This allowed us to improve the properties of hydrogel even at low weight concentration of materials where gelation is particularly challenging.
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Defined hydrogel microenvironments for optimized neuronal cultureSeidlits, Stephanie Kristin 16 February 2015 (has links)
Three-dimensional (3D) in vitro culture systems that provide controlled, biomimetic microenvironments would be a significant technological advance for both basic cell biology research and the development of clinical therapeutics (e.g., as in vivo cell delivery constructs). A variety of signals determine cell phenotype, including those from soluble factors, immobilized biomolecules, mechanical substrates, and culture geometry. My research seeks to create hydrogel culture systems that incorporate these signals in a defined, controllable manner. Specifically, I have focused on developing hydrogels based on the extracellular matrix (ECM) component hyaluronic acid (HA) with precisely specified mechanical, chemical and geometrical microenvironments. For example, the mechanical environment presented by HA hydrogels was tuned to span the threefold range measured for neonatal brain and adult spinal cord by modifying HA with varying numbers of photocrosslinkable methacrylate groups. These hydrogels were used to evaluate the effects of mechanical properties of a 3D culture paradigm on the differentiation of ventral midbrain-derived neural progenitor cells (NPCs) and results demonstrated that the mechanical properties of these scaffolds can assert a defining influence on differentiation. In addition, whole fibronectin was incorporated into HA hydrogels as an adhesive factor to encourage angiogenesis in 3D cultures, as interplay between endothelial cells and neurons is an important determining factor during NPC development and axonal regeneration after injury. To create spatially defined neuronal cultures in three-dimensions, multiphoton excitation (MPE) was used to photocrosslink protein microstructures within HA hydrogels. This method can be used to create complex, 3D architectures that provide both chemical and topographical cues to direct cell adhesion and guidance on size scales relevant to in vivo environments. Using this approach, both dorsal root ganglion cells (DRGs) and hippocampal NPCs could be guided along user-defined, 3D paths. In future studies, these strategies can be combined into a single hydrogel to create a culture microenvironment with multiple types of highly specified cues (i.e., chemical, topographical, and mechanical). / text
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