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1	Preparation and Characterization of Multifunctional Stationary Phases for Multimode Separations Wijekoon, Asanka 24 February 2010 (has links) No description available. Analytical Chemistry Atoms and Subatomic Particles Biochemistry Biogeochemistry Chemical Engineering Chemistry Materials Science HPLC NMR Reversed Phase IEX HILIC Motional Dynamic Heterogeneity
2	Purification of His-tagged Proteins Using WorkBeads 40 TREN as a Pre-Treatment Step Prior Loading Sample onto IMAC Resins with the Purpose to Enhance Performance Thorsén, Jenny January 2021 (has links) This work is the result of evaluating a novel strategy for the purification of recombinant His-tagged proteins. Proteins purified in this study were the E. coli translational proteins IF-3, RF-1, and RFF. The study aimed to analyse the potential of using Bio-Works WorkBeads™40 TREN, a multimodal anion ion exchange chromatography resin, as a pretreatment step upstream an immobilized metal ion chromatography (IMAC) resin to enhance performance efficiency of His-tagged protein purification. The method demonstrated here shows potential for anyone seeking to increase the purity of His-tagged protein purification or to introduce an effective purification procedure by replacing a polishing step downstream IMAC with WorkBeads 40 TREN upstream IMAC. The latter contributing to guard the IMAC column from heavy bioburden. This study showed that running WorkBeads 40 TREN prior IMAC captures impurities and removes 97-98 % more dsDNA compared to direct IMAC. WorkBeads 40 TREN is therefore highly advantageous to include early in a purification process to remove protein binding DNA fragments. Moreover, WorkBeads 40 TREN increases purity in the final product by capturing more host cell proteins than when running direct IMAC. This concept is general and WorkBeads 40 TREN could be used upstream a variety of resins such as Protein A and RPC. Purification IMAC SEC IEX His-tag Protein Resin Bio-Works Bradford ELISA DNA host cell DNA host cell proteins separation Natural Sciences Naturvetenskap Biochemistry and Molecular Biology Biokemi och molekylärbiologi Bioengineering Equipment Bioteknisk apparatteknik

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Preparation and Characterization of Multifunctional Stationary Phases for Multimode Separations

Purification of His-tagged Proteins Using WorkBeads 40 TREN as a Pre-Treatment Step Prior Loading Sample onto IMAC Resins with the Purpose to Enhance Performance