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This work deals with the determination of the heat transfer coefficient in small channels with a rotating wall. The research part of the thesis is focused on specific geometry, namely labyrinth seals used in rotary machines. Existing criteria equations determining heat transfer coefficients and other parameters in these specific geometries are described. The central part describes the built experimental device for the determination of values of heat flows and heat transfer coefficients on both stator and rotor for given airflow and rotor speed. The following section is devoted to presenting the results of the performed experiments with the corresponding criteria equations. The next part describes the basic mathematical models of inverse heat conduction problems. Calculations of heat flux using these inverse methods are performed and compared with reference heat flux sensors.