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Search for the Nuclear Localization Signal of Ime4Hernandez, Christian Monroy 01 May 2018 (has links)
Ime4 is the catalytic subunit of a conserved methyltransferase (MTase) complex found in yeast, S. cerevisiae. This complex is responsible for creating the RNA modification N6- methyladenosine (m6A), the most common post-transcriptional modification in higher eukaryotes. There is evidence to suggest that m6A is an important mediator of gene expression control within the cell and has been associated with a diverse array of phenotypic effects, notably as a conserved determinant of cell fate. The MTase complex is known to be a nuclear protein, the compartment where it is believed to carry out most of its methylation activity. Recently, the nuclear localization signals (NLS) of the subunits of the human MTase complex were experimentally identified, whereas the NLSs of the yeast MTase complex remain unknown. Here, we have experimentally identified the amino acid sequence 517RKYQEFMKSKTGTSHTGTKKIDKK540, located within the C-terminal region, as a putative bipartite NLS for Ime4.
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Localization of Ime4 in Saccharomyces cerevisiaeGhimire, Jenisha 01 May 2012 (has links)
One lesser-known but universal post transcriptional modification carried out in yeast and higher eukaryotes is the methylation of mRNA, as mediated by the Ime4 protein and its orthologs. Ime4 protein is essential for sporulation in yeast cells and for viability of higher eukaryotic cells. The precise locations of the Ime4 protein and the functions of the methylated mRNA are still largely unknown. Whereas Ime4 protein is believed to be exclusively nuclear in higher eukaryotes, we have observed the yeast Ime4 protein in the nucleus, in the cytosol and within cytosolic particles. These observations suggest that Ime4 could be a shuttling RNA binding protein, playing roles in the cytosol as well as the nucleus. As a first step to examining this idea, we tested the hypothesis that the punctuate cytosolic particles formed by Ime4 are P bodies. P bodies are transient aggregates of proteins and RNAs that form as a result of stresses such as glucose deprivation. This experiment was carried out using fluorescence microscopy using Ime4 tagged with GFP (green fluorescent protein) and the known P -body proteins Edc3, tagged with mCherry. We expected that if the proteins thus produced localized in the same place in the yeast cell, we could then deduce that Ime4 is present in P-bodies. We observed that Ime4 and Edc3 did not colocalize in the majority of cells, and thus concluded that the Ime4 granules are not P-bodies. However, our experiments showed instances of Ime4 signals near or around the P-bodies in some cells. Hence, the Ime4-containing aggregates are not likely to be P-bodies but could rather represent a different type of granule.
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Protein Interactions in mRNA Methylation ComplexesAlqara, Yazan Ali 01 May 2013 (has links)
Experiments were performed to test sequence and structural specific interactions of proteins with a conserved RNA modification enzyme, which is known as Ime4 in yeast and Mettl3 in mammals. Ime4 methylates N6-adenosine bases on mRNA molecules. The goal of this project is to gain direct insights into how novel proteins interact with Ime4 to form the methyltranferase (MTase) complex and to identify proteins that are essential for Ime4 activity. It has been recognized that there are two proteins that interact within the Ime4 complex, which are known as Mum2 (a cytoplasmic protein essential for meiotic DNA replication within yeast) and Slz1 (a transcription factor). We hypothesize that the N-terminal domain of Ime4 is the location of binding of the aforementioned proteins in this complex. Similarly, we tested whether the human ortholog of Ime4 (Mettl3) forms an analogous complex that includes an ortholog of Mum2, known as WTAP, and its binding partner WT1. The major approaches include in vivo genetic assays in yeast to test protein-protein interactions and the use of recombinant DNA technology to construct fusion genes/deletions. The results demonstrate that Mum2 interacts with a specific, non-conserved region in the Ime4 N-terminal domain. Furthermore, we discovered a new binding partner, Ygl036w, which also interacts with Ime4. Currently, several experiments are being carried out with the Mettl3 complex and its hypothesized protein binding partners to assess the interactions of this complex.
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Localization and Mutational Analysis of the Nuclear and Aggregation-Prone Ime4 Protein in Saccharomyces cerevisiaeDehon, Patricia M 15 December 2012 (has links)
In Saccharomyces cerevisiae, Ime4 is a protein that is induced during meiosis and has a primary role in regulating sporulation in starving diploids. One function of Ime4 is methylation of adenosine residues within mRNA transcripts. Recent studies have shown Ime4 to be induced in haploids during the mating response, although its role in mating has not been determined. In this report, I identify the subcellular localization of Ime4 during the mating response through treatment with alpha factor. A plasmid containing IME4-GFP under the control of the medium strength promoter CYC1 was created in order to express the protein in a controlled manner. Lastly, mutational analysis was conducted to determine which regions of the protein were necessary for its nuclear localization, aggregation, and sporulation function.
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Role of Ime4 Protein in PHO Regulon of S.cerevisiae.Ghimire, Jenisha 11 August 2015 (has links)
In the yeast Saccharomyces cerevisiae, the IME4 methyltransferase, interacts genetically with methyl binding protein, Pho92, to affect the expression of PHO regulon target genes. Cells mutant in IME4 or PHO92 show increases in the RNA abundance of PHO regulon target genes. The increase in the RNA abundance of the PHO regulon target genes is not additive in the cells double mutant in IME4 and PHO92. Hence, Ime4 and Pho92 interact in a single pathway in PHO regulon. Surprisingly, cells overexpressing IME4 and MUM2 shows increase in some PHO regulon target genes, indicating that IME4 affects the PHO regulon target genes through multiple mechanisms in different conditions. A promoter swap experiment revealed that one of the PHO regulon mRNAs that codes for phosphatase, PHO5, is a direct target of Ime4. Further experiments are required to examine whether the same is true for all PHO regulon mRNAs.
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Effects of a putative Reb1 protein binding site on IME4 sense and antisense transcription and sporulation in Saccharomyces cerevisiaeRamsay, Milele 20 December 2009 (has links)
Genome transcription is much more widespread than has been traditionally thought because our view of a "gene" or "transcription unit" has changed dramatically over the past 4 to 5 years with the identification of many different non-coding ribonucleic acids. In the yeast, Saccharomyces cerevisiae, meiosis and sporulation are an important part of the life cycle and IME4 gene expression is required for these processes. IME4 sense transcript levels of expression are influenced by the level of its complementary non-coding antisense strand by mechanisms that are currently unknown. The a1-alpha2 heterodimer binding in the downstream 3' region of IME4 is one component required for repression of IME4 antisense transcription. However, this thesis shows that the general regulatory protein Reb1 is also required in this system. Reb1 involvement is most likely to create a nucleosome-free zone in the promoter region of the IME4 antisense strand therefore contributing to transcription.
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