京都大学 / 0048 / 新制・課程博士 / 博士(エネルギー科学) / 甲第22798号 / エネ博第412号 / 新制||エネ||79(附属図書館) / 京都大学大学院エネルギー科学研究科エネルギー応用科学専攻 / (主査)教授 平藤 哲司, 教授 土井 俊哉, 教授 馬渕 守 / 学位規則第4条第1項該当 / Doctor of Energy Science / Kyoto University / DFAM

Hierarchical systems that integrate nano- and macroscale structural elements can offer enhanced stability over traditional immobilization methods. Microparticles were synthesized using interfacial assembly of lipase with (CLMP-N) and without (CLMP) nanoparticles into a crosslinked polymeric core, to determine the impact of the highly ordered system on lipase stability in extreme environments. Kinetic analysis revealed the macrostructure significantly increases the turnover rate (kcat) following immobilization. The macrostructure also stabilized lipase at neutral and basic pH values, while the nanoparticles influenced stability under acidic pH conditions. A greener solvent, choline chloride and urea, was applied to produce sugar ester surfactants. Microparticles exhibited decreases in the turnover rate (kcat) and catalytic efficiency (kcat/Km) following exposure, but retained over 60% and 20% activity after exposure at 50 ºC and 60 ºC, respectively. CLMP and CLMP-N outperformed the commercially available lipase per unit protein in the production of sugar esters. The utilization of greener solvent systems with hierarchical immobilized enzyme systems has the potential to improve processing efficiency and sustainability for the production of value-added agricultural products.

Content: Nowadays, tannery pollution is of great concern worldwide. The unhairing and fiber bundle-opening processes contribute the majority of the pollution by the use of sodium sulfide and calcium hydroxide, which were proposed to be replaced by neutral protease combined with (potassium chloride) KCl, (lithium bromide)LiBr/1-allyl-3-methylimidazolium chloride([AMIm]Cl) in the present work. Proper amount of KCl can speed up the unhairing with the grain not destroyed by the neutral protease. Four methods for unhairing and fiber bundle-opening were used as follows: 1. Two steps in different floats as KCl/neutral protease unhairing, followed by LiBr/[AMIm]Cl for fiber bundle-opening; 2. Two steps in different floats as neutral protease unhairing, followed by LiBr/[AMIm]Cl for fiber bundle-opening; 3. One step in the same float as neutral protease for unhairing firstly and then LiBr/[AMIm]Cl for fiber bundle-opening. 4. One step in the same float as neutral protease/KCl for unhairing firstly and then LiBr/[AMIm]Cl for fiber bundle-opening. It was found that using neutral protease/KCl solution for unhairing and LiBr/[AMIm]Cl solution for fiber bundle-opening is the best in fiber bundle-opening at the liming process. Besides, all the methods used here are better than the traditional liming processes (C) from viewpoints of unhairing and fiber bundleopening. Take-Away: 1. The addition of potassium chloride can improve the stability of neutral protease solution. 2. The catalytic efficiency of the enzyme was improved because of adding potassium chloride can enhance the contact rate of neutral protease with substrate. 3. Ionic liquids have less hydrolysis of collagen and larger dissolution of polysaccharides.

This work deals with electrolytes and ionic liquids for Li-ion batteries. Following interaction of electrolytes and ionic liquids to electrodes material. In the theoretical part attention is focused on the description of battery electrolytes and ionic liquids for lithium-ion batteries.

The theoretical part of diploma thesis „Selection of a suitable type of flux for wave soldering”, is devoted to problematic of fluxes, flux residues and wave soldering. It introduces reader with requirements of company HC electronics and with selection of a suitable types of fluxes for testing. It also contains theoretical comparison and design of flux testing methodologies. The practical part of the diploma thesis is focused on the selection of a suitable flux for the company HC electronics by performing practical tests according to the previously proposed methodologies, which include wetting balance test method, surface insulation resistance measurement, copper mirror test, soldering test of PCB and ROSE method.

The master's thesis deals with experimental study of tribological characteristics of smart fluids. Smart fluids are substances in liquid state reacting to the presence of magnetic or electric field by change in rheological properties. For possible application in devices using conventional lubricants is necessary to choose suitable smart fluid and study the influence of excitation on formation of lubricating layer, friction and wear. Comprehensive description of excitation influence is executed using three experimental devices and theoretical model for measurements parameters specification. Assessed are lubricant film thickness, friction coefficient and wear under smart fluid activation in non-conformal contact. Results show significant observable influence of smart fluids excitation on all assessed aspects. Understanding the mechanisms of smart fluids excitation can be a key step in development of intelligent devices with active external control of lubricant behaviour and character, that could lead to maintenance cost reduction and effectivity improvement.

This thesis deals with design and implementation of mobile application for iOS and Android operating systems using tools for mobile application development. Ionic 4 was chosen for implementation, which is a set of tools that can be used to develop software, using Angular, HTML and SASS.

京都大学 / 0048 / 新制・課程博士 / 博士(エネルギー科学) / 甲第22795号 / エネ博第409号 / 新制||エネ||78(附属図書館) / 京都大学大学院エネルギー科学研究科エネルギー基礎科学専攻 / (主査)教授 萩原 理加, 教授 佐川 尚, 教授 野平 俊之 / 学位規則第4条第1項該当 / Doctor of Energy Science / Kyoto University / DFAM

京都大学 / 0048 / 新制・課程博士 / 博士(エネルギー科学) / 甲第22860号 / エネ博第413号 / 新制||エネ||79(附属図書館) / 京都大学大学院エネルギー科学研究科エネルギー基礎科学専攻 / (主査)教授 萩原 理加, 教授 佐川 尚, 教授 野平 俊之 / 学位規則第4条第1項該当 / Doctor of Energy Science / Kyoto University / DFAM

Isoporous membranes are attractive for the regulation and detection of transport at the molecular level. A well-defined asymmetric membranes from diblock copolymers with an ordered nanoporous membrane morphologies were fabricated by the combination of block copolymer self-assembly and non-solvent-induced phase separation (NIPS) technique. This is a straightforward and fast one step procedure to develop integrally anisotropic (“asymmetric”) membranes having isoporous top selective layer. Membranes prepared via this method exhibit an anisotropic cross section with a thin separation layer supported from underneath a macroporous support. These membrane poses cylindrical pore structure with ordered nanopores across the entire membrane surfaces with pore size in the range from 20 to 40 nm. Tuning the pore morphology of the block copolymer membranes before and after fabrication are of great interest. In this thesis, we first investigated the pore morphology tuning of asymmetric block copolymer membrane by complexing with small organic molecules. We found that the occurrence of hydrogen-bond formation between PS-b-P4VP block copolymer and –OH/ –COOH functionalized organic molecules significantly tunes the pore morphology of asymmetric nanoporous membranes. In addition, we studied the complexation behavior of ionic liquids with PS-b-P4VP block copolymer in solutions and investigated their effect on final membrane morphology during the non-solvent induced phase separation process. We found that non-protic ionic liquids facilitate the formation of hexagonal nanoporous block copolymer structure, while protic ionic liquids led to a lamella-structured membrane. Secondly, we demonstrated the catalytic activity of the gold nanoparticle-enhanced hollow fiber membranes by the reduction of nitrophenol. Also, we systematically investigated the pore morphology of isoporous PS-b-P4VP using 3D imaging technique. Thirdly, we developed well-distributed silver nanoparticles on the surface and pore walls of PS-b-P4VP block copolymer membranes and then investigated the biocidal activity of the silver nanoparticles grown membranes. Finally, a novel photoresponsive nanostructured triblock copolymer membranes were developed by phase inversion technique. In addition, the photoresponsive behavior on irradiation with light and their membrane flux and retention properties were studied.