In recent years the 6.67 GHz masing transition of CH3OH has proven to be a superior tracer of massive star formation (see Minier 2001). Maser sites often occur in proximity to UCHII regions, however, up to 75 per cent of sites have no detectable radio counterpart (Walsh 1998) and are instead hypothesised to trace the less evolved 'hot molecular core' phase of stellar evolution. This has been confirmed for a only handful of well known sources (e.g., Cesaroni 1994). Presented here are the results of multi-species molecular line observations towards warm, dusty clumps, undertaken with the goal of investigating the relationship between hot cores, UCHII regions and CH3OH masers. Data from the 22-m Mopra telescope is used extensively in this thesis and substantial efforts were made to calibrate the brightness temperature scale. Measurements conducted on SiO masers and planets show that the beam pattern is divided into a Gaussian main beam plus an inner error lobe, which in 2004 contained 1/3 of the power in the main beam. Full-width half-maximum beam sizes were measured from the data and the beam efficiencies were derived for the years 2000-2004. A 3-mm wavelength molecular line survey was conducted, using Mopra, towards 83 massive star-forming clumps associated with CH3OH masers. Emission from the transitions 13CO (1-0), N2H+ (1-0), HCO+ (1-0), HCN (1-0) and HNC (1-0) was detected towards 82 sources (99 per cent), while CH3OH emission was detected towards 78 sources (94 per cent). The warm gas tracer CH3CN was observed specifically to search for hot core chemistry, and was detected towards 58 sources (70 per cent), confirming that CH3OH masers are excellent tracers of hot cores. CH3CN is found to be brighter and more commonly detected towards masers associated with UCHII regions compared to 'isolated' masers. That CH3CN is detected towards isolated maser sources strongly suggests that these objects are internally heated. The molecular line data have been used to derive rotational temperatures and chemical abundances in the clumps and these properties have been compared between sub-samples associated with different indicators of evolution. In particular, CH3OH is found to be brighter and more abundant in UCHII regions and in sources with detected CH3CN, and may constitute a crude molecular clock in single dish observations. Gas-kinematics were analysed via asymmetries in the HCO+ line profiles. Approximately equal numbers of red and blue-skewed profiles, indicative of inward or outward motions, respectively, are found among all classes of object. Bolometric luminosities were derived via greybody fits to the sub-millimetre and mid-infrared spectral energy distributions, and an empirical gas-mass to luminosity relation of L proportional to M^0.68 was fit to the sample. This is a considerably shallower power law than L proportional to M^3 for massive main-sequence stars. In the mid-infrared, 12 sources were identified as 'infrared dark clouds' (IRDCs). Such objects have been hypothesised as precursors to the hot core phase of evolution, however, we find these sources have greater linewidths and rotational temperatures than the bulk of the sample, and one contains an embedded HII region The filamentary star forming region NGC3576 was also investigated via a molecular line and 23 GHz continuum mapping survey, utilising the ATCA, Mopra and Tidbinbilla telescopes. The results of these observations provide detailed information on the morphology, masses, kinematics, and physical and chemical conditions along the cloud. Analysis of NH3 data has revealed that the temperature and linewidth gradients exist in the western arm of the filament. Values are highest near to the central HII region, indicating that the embedded cluster of young stars is influencing the conditions in the bulk of the gas. Six new H2O masers were detected in the arms of the filament, all associated with clumps of NH3 emission. Star formation is clearly underway, however, clump masses range from 1 to 128 solar masses, possibly too low to harbour very massive stars. The lack of detected 23 GHz continuum emission in the arms supports this assertion.

Alors que les galaxies évoluent, leur milieu interstellaire (MIS) s’enrichit continuellement en métaux, et cet enrichissement influence la formation d’étoiles. Les galaxies naines de faible métallicité de l’Univers Local sont les candidates idéales pour étudier l’influence de cet enrichissement en métaux sur les propriétés du MIS des galaxies et nous donne un aperçu des processus d’enrichissement et de formation stellaire dans des conditions proches de celles trouvées dans les systèmes pauvres en métaux de l’Univers primordial. Des études précédentes ont montré que le MIS des galaxies naines pose un certain nombre d’énigmes en terme d’abondance des grains, de composition de la poussière et même des processus d’émission en infrarouge lointain (FIR). Cependant, ces études étaient limitées à la poussière chaude émettant à des longueurs d’onde plus courtes que 200 micromètres et étaient effectuées sur un petit nombre de galaxies. Grâce à une sensibilité et une résolution améliorées dans les domaines FIR et submillimétriques (submm), Herschel nous donne une vue nouvelle sur les propriétés de la poussière froide dans les galaxies et nous permet d’étudier les galaxies les plus pauvres en métaux de manière systématique. Dans ce travail, je mène une étude des propriétés des poussières dans les galaxies naines et compare avec des environnements plus riches en métaux, pour aborder la question de l’impact de la métallicité sur les propriétés de la poussière. La nouveauté de ce travail réside dans le fait que les galaxies naines sont étudiées de manière systématique, nous permettant d’accéder aux, et de quantifier les propriétés générales représentatives de ces systèmes. Cette étude est conduite sur toute la gamme de longueurs d’onde infrarouge (IR)-submm, avec les nouvelles observations en FIR/submm d’Herschel, ainsi que des données Spitzer, WISE, IRAS, et 2MASS. Nous complétons ces données avec des mesures en domaine submm de télescopes au sol comme APEX ou le JCMT, pour étudier la présence et les caractéristiques de l’excès submm dans mon échantillon de galaxies. Je collecte aussi les données HI et CO pour accéder aux propriétés du gaz dans ces galaxies et étudier l’évolution du rapport en masse gaz-sur-poussière (G/D) avec la métallicité. Notre étude révèle des propriétés de poussière différentes dans les environnements de faible métallicité que celles observées dans des systèmes plus riches en métaux (par exemple, une poussière globalement plus chaude). Une émission en excès par rapport aux modèles utilisés, apparait souvent aux alentours de 500 micromètres, menant à d’importantes incertitudes sur les propriétés de la poussière, notamment sur la masse de poussière. Les excès les moins importants peuvent cependant être expliqués en utilisant une autre composition pour la poussière, avec des grains plus émissifs. Traceur idéal de l’état d’évolution chimique d’une galaxie, le G/D est en fait bien plus grand que ce que l’on pourrait attendre si l’on considère un modèle simple d’évolution chimique. Interprétée avec des modèles d’évolution chimique plus complexes, incorporant des processus de croissance des grains et/ou une formation d’étoiles épisodique, la relation entre le G/D et la métallicité, ainsi que sa dispersion, peuvent être expliquées par la grande variété d’environnements que nous considérons dans notre étude. / As galaxies evolve, their Interstellar Medium (ISM) becomes continually enriched with metals, and this metal enrichment influences the subsequent star formation. Low metallicity dwarf galaxies of the local Universe are ideal candidates to study the influence of metal enrichment on the ISM properties of galaxies and gives us insight into the enrichment process and star formation under ISM conditions that may provide clues to conditions in early universe metal-poor systems. Previous studies have shown that the ISM of dwarf galaxies poses a number of interesting puzzles in terms of the abundance of dust grains, the dust composition and even the FIR emission processes. However these studies were limited to the warmer dust emitting at wavelengths shorter than 200 microns and were done only on a small number of dwarf galaxies. Thanks to its increased sensitivity and resolution in FIR and submillimeter (submm) wavelengths, Herschel gives us a new view on the cold dust properties in galaxies and enables us to study the lowest metallicity galaxies in a systematic way. In this work, I carry out a study of the dust properties in dwarf galaxies and compare with more metal rich environments, in order to address the question of the impact of metallicity on the dust properties. The novelty of this work lays in the fact that dwarf galaxies are studied here in a systematic way, enabling us to derive and quantify the general properties that are representative of these systems. This study is conducted over the full IR-to-submm range, using new FIR/submm Herschel observations, Spitzer, WISE, IRAS and 2MASS data. We complete this set of data with longer submm measurements from ground-based facilities such as APEX and JCMT to study the presence and characteristics of the submm excess in my sample of galaxies. I also collect Hi and CO data to access the gas properties of the galaxies and study the evolution of the G/D with metallicity. Our study reveal different dust properties in low-metallicity environments than that observed in more metal-richs systems (e.g., an overall warmer dust component). An excess submm emission is often apparent near and/or beyond 500 microns rendering large uncertainties in the dust properties, even for something as fundamental as dust masses. Some of the smallest excesses can be explained by using another dust composition with more emissive grains. Ideal tracer of the chemical evolutionary stage of a galaxy, the gas-to-dust mass ratios (G/D) is found to be much higher than what is expected by simple chemical evolution models. Interpreted with more sophisticated chemical evolution models, including dust growth in the ISM and/or episodic star formation, the relation of the G/D with metallicity and its scatter can be explained by the wide variety of environments we are considering.