This study investigates the relationship between Organizational Behavior (OB), IT-business strategic alignment (ITBSA), and Firm Performance (FP) within the context of the Jordanian maritime industry. Through a survey conducted among companies in the industry, data were collected to assess the constructs of OB, ITBSA, and FP. The analysis of the data revealed significant findings supporting the positive impact of OB on FP, suggesting that effective organizational behavior practices contribute to improved firm performance. Furthermore, the study found that OB positively influences ITBSA, indicating that a strong organizational behavior framework promotes the alignment between IT and business strategies. Additionally, the results demonstrate that ITBSA directly affects FP, emphasizing the importance of strategic alignment in driving favorable performance outcomes. The study also identified that ITBSA acts as a mediator between OB and FP, underscoring its role in facilitating the relationship between organizational behavior and firm performance. These findings offer valuable insights into the dynamics of the Jordanian maritime industry and emphasize the significance of fostering a conducive organizational behavior environment and aligning IT with business strategies. The implications of these findings for both theory and practice are discussed, along with suggestions for future research directions to further enhance our understanding of the relationships between OB, ITBSA, and FP in the maritime industry context.