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Ice nuclei and convective storms.Isaac, George A. January 1972 (has links)
No description available.
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Cryostability of large unilamellar vesicles in relation to the effect of cryoprotective agents on ice matrixSiow, Lee Fong, n/a January 2008 (has links)
Freeze-injury at the plasma membrane level has been identified as being crucial for the survival of living matter. Since plasma membranes consist of several micro domains that make the structure rather complex, this study attempted to use simple model membranes to investigate the changes of phospholipid bilayers at sub-zero temperatures. Egg yolk L-α-phosphatidylcholine (EPC) and 1, 2-dipalmitoyl-rac-glycero-3-phosphocholine (DPPC) that mimic plasma membranes in their unique ways were used to prepare large unilamellar vesicles (LUV), which were the model membranes of this study.
At cooling rates of 0.5 and 10�C/min, LUV were freeze-concentrated in the unfrozen matrix as a result of the advancing extraliposomal ice front and the decreasing phase volume of the unfrozen matrix, both of which led to membrane lesion. At the slow cooling rate of 0.5�C/min, an additional freezing stress imposed by the osmotic gradient across the bilayers, due to the increase of solute concentration in the unfrozen matrix, promoted leakage of LUV.
The gel-liquid crystal phase transition temperature of phospholipids played an important role in determining if the LUV could withstand freezing stress when the LUV were held at a defined sub-zero temperature for a given period of holding time. EPC LUV were more leaky than DPPC LUV when they were held at the high sub-zero temperatures and their leakage increased with increasing holding time. The leakiness of EPC LUV could be related to the fluid and deformable nature of the EPC above its phase transition temperature. In contrast, DPPC LUV with a higher gel-liquid crystal phase transition temperature compared to EPC may become increasingly fragile at lower sub-zero temperatures, which led to the increase of leakage when the DPPC LUV were held at the lower sub-zero temperatures. These results indicated that the determination of the fatty acid profile of the plasma membranes was essential to aid in developing the most suitable holding temperature and time during the cryopreservation of biological specimens. Adding to the integrity of LUV that depended on the gel-liquid crystal phase transition temperature of phospholipids, intraliposomal ice formation also depended on the phase transition temperature of phospholipids. Intraliposomal ice formation was only observed for DPPC LUV but not for EPC LUV.
In addition to the extraliposomal ice formation, other physical changes such as the eutectic crystallization of sodium chloride (NaCl) and ice mixture on the stability of LUV were also investigated. The eutectic crystallization of NaCl/ice mixture was governed by the intra- and extraliposomal distribution of NaCl and was more likely to occur at the physiological NaCl concentrations compared to lower NaCl concentrations. The eutectic crystallization of NaCl/ice mixture further increased the leakage of LUV.
The understanding of the freezing behaviour and the mechanisms of freeze-injury of LUV allowed the use of the current model membranes for further investigations of the cryoprotective actions of cryoprotective agents (CPA). Partial phase diagrams of sugar-salt-water, dimethyl sulfoxide (DMSO)-salt-water and ethylene glycol (EG)-salt-water systems that resembled extraliposomal solute compositions were constructed and the phase volume of ice and unfrozen matrix was estimated from the freezing curves. Ice reduction was the major mechanism by which the non-permeable and permeable CPA protected the LUV from freeze-injury. Other cryoprotective mechanisms of the non-permeable and permeable CPA through the dilution and spacing out of the LUV in the unfrozen matrix as well as the suppression of the eutectic crystallization of NaCl/ice mixture were not ruled out. Non-permeable CPA were more effective in preventing leakage of DPPC than EPC LUV. Unlike the non-permeable CPA, permeable CPA were more effective for EPC than DPPC LUV that had been subjected to freezing and thawing processes. At room temperature, however, DMSO and EG were detrimental to the stability of DPPC LUV. The choice of CPA is strictly dependent on the type of phospholipids that varied in their acyl chain length and phase transition temperature.
In summary, this study provides insights of the freeze-injury of LUV and the cryoprotective mechanisms of the non-permeable and permeable CPA which are beneficial to the field of cryopreservation that often depends on empirical trial and error methods. By integrating a comprehensive molecular-based understanding, an optimal cryopreservation procedure could be designed.
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A study on the hyperactive antifreeze proteins from the insect Tenebrio molitorChoi, Young Eun. January 2007 (has links)
Thesis (M.S.)--Ohio University, November, 2007. / Title from PDF t.p. Includes bibliographical references.
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A comparison of theory with laboratory and field observations of wave propagation in grease ice /Newyear, Karl D. January 1997 (has links)
Thesis (Ph. D.)--University of Washington, 1997. / Vita. Includes bibliographical references (leaves [121]-127).
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Aerosol scavenging by natural ice crystals an experimental study /Sauter, David P. January 1984 (has links)
Thesis (M.S.)--University of Wisconsin--Madison, 1984. / Typescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 99-102).
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Ice nuclei and convective storms.Isaac, George A. January 1972 (has links)
No description available.
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O17 spin-lattice relaxation solid state NMR studies of pure and doped icesGroves, Ronald William. January 2002 (has links)
Thesis (Ph. D.)--Ohio State University, 2002. / Title from first page of PDF file. Document formatted into pages; contains xx, 128 p.; also contains graphics. Includes abstract and vita. Co-advisors: Charles H. Pennington and James V. Coe, Dept. of Chemistry. Includes bibliographical references (p. 124-128).
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The effect of ice crystal shape on aircraft contrailsMeza Castillo, Omar Enrique. January 1900 (has links)
Thesis (Ph. D.)--West Virginia University, 2010. / Title from document title page. Document formatted into pages; contains xvii, 124 p. : ill. (some col.). Includes abstract. Includes bibliographical references (p. 116-124).
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Ice lens formation and frost heave from a thermodynamic rheologic perspective.Smith, Sharon Lee, Carleton University. Dissertation. Geology. January 1992 (has links)
Thesis (Ph. D.)--Carleton University, 1992. / Also available in electronic format on the Internet.
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Characteristics and formation of precipitation over the Kananaskis Emergency Site during March and April 2015Hung, Ida 15 December 2016 (has links)
A field campaign was conducted in March and April 2015 in the Kananaskis Valley of Alberta to investigate the formation and characteristics of ice crystals and solid precipitation particles in the lee of the Rocky Mountains. This thesis examines data from 11 storms producing mainly light precipitation within generally sub-saturated surface and near-surface conditions. Instruments utilized include soundings, radar images, and surface observations, but the focus is on the analysis of 1,183 microphotographic images of precipitation particles. The particles (diameters up to 24 mm) were placed into 12 categories with rimed irregular snow particles being most common. Unrimed and rimed particles were commonly (14% of images) observed simultaneously and 62% of particles were rimed. Rimed, dense particles were less likely to sublimate before reaching the surface in the dry sub-cloud region that was at least partially a result of the air aloft being directed mainly towards the east and ‘downslope’. / February 2017
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