The presented thesis includes three related projects, that are linked by a common interest in the evolution of eukaryotic organelles and machineries that import proteins into these compartments. The first project considers the possibility of peroxisomes (eukaryotic organelles known in aerobic organisms) being conserved in two related anaerobic protists: a free-living amoeba Mastigamoeba balamuthi and a parasite Entamoeba histolytica. The most important hint for the presence of peroxisomes was the discovery of proteins that are homologous to known components of the peroxisomal protein import machinery. The second project aims to characterize the unknown protein translocase of the inner membrane (TIM) in the mitosomes (extremely reduced mitochondria) of an anaerobic protozoan Giardia intestinalis. We have discovered an important subunit of the mitosomal translocase (Tim44), which usually tethers the Hsp70/PAM (presequence translocase-associated motor) complex to the TIM translocon. The last project shows that the protein translocase of the outer mitochondrial membrane in trypanosomatids is related to a typical eukaryotic channel Tom40. This finding is important because the absence of Tom40 was previously considered an ancestral feature of trypanosomatids.

Oligopoly behaviour by domestic firms faced with foreign competition in a small open economy is examined in the context of a market for differentiated products. This paper concentrates on the responsiveness of import flows to import price in the context of trade with imperfect competition. The empirical work analyses the behaviour over time of the interaction between domestic industry prices and domestic costs as well as foreign competitors' prices. A structural model is employed for estimation purposes with consumer demand derived from a CES (constant elasticity of substitution) utility function of domestic and foreign composites of goods. Domestic firms are assumed to face Leontief production functions and maximise profit independently subject to their conjectures about the reactions of rivals. Firm behaviour is modelled using conjectural variations to identify market power, distinguishing two models of oligopoly, namely, Cournot and Bertrand conjectural variations. This leads to the econometric specification of pricing, import and budget share equations consistent with oligopolistic equilibrium. The interrelationship between the budget share equations and the price-cost margin provides encompasses either Cournot or Bertrand conjectural variations. The econometric specification is applied to each of the two digit Australian manufacturing industries using quarterly data covering the period from 1984 to 2000. Results of the industrial behaviour indicate that industries that produce consumer products are generally react to price movements The classification of industry 21 to 24 is more proximate to consumer products as compared to higher industrial numbering. The regression results for industry 25 to 28 suggest quantity reactions. This is in line with the nature of the products produce by these industries, which are heavy industrial manufacturing products. / The elasticity with respect to foreign price is distinguished between the "partial" and the "total" effect. The partial elasticity of import demand ranges from .6205 to 4.9497, while the total elasticity of import demand ranges from .6505 to 19.8132. The elasticity of demand ranges from .0191 for Wood and Paper Product manufacturing to 3.4093 for Food, Beverage and Tobacco manufacturing.

The aim of the diploma thesis was to handle the recommendation, which would serve as a useful tool for businesses with the commercial intent on importing goods from China. It was necessary to recognize secondary data and gain primary data in order to comply it. The methodology of the thesis was divided in three parts. In the analytic part, the collecting of secondary information was carried out through the study of literature. The books and the Internet sources of Czech and foreign origin were used. On the basis of this information the literary recherché was written and then the analysis of the foreign trade of the Czech Republic and China was carried out. The synthetic part dealt with the preparation and the realization of the field investigation and its processing. To obtain the primary information the method of controlled conversation was used. The realization of field investigations was carried out in the company of ETA a.s. in Hlinsko. The subsequent evaluation of the field investigation was carried out through the drafting of a practical example, where the company's business cooperation with the Chinese enterprises to the ETA was pointed out. The last part of the application was devoted to the processing of recommendations of good practice when dealing with China, which presented a firm from the Czech Republic as the importer, and China's venture as the exporter, according to the example of the ETA company. The aim of the recommendation, which was established in the framework of this thesis, is to become a useful tool for businesses that have minimal experience with foreign trade and at the same time they would like to establish business cooperation with Chinese companies.

No description available.

Plants have evolved sophisticated defence systems against pathogen infection. Initiation of induced defence signalling often involves specific recognition of invading pathogens by the products of specialized host Resistance (R) genes. Consequently, the pathogen is stopped at the site of infection. A unique dominant mutant in Arabidopsis thaliana, snc1, constitutively expresses pathogensis-related (PR) genes and exhibits enhanced resistance to bacterial and oomycete pathogens. SNC1 encodes an R-gene – a single amino acid change renders this protein constitutively active without interaction with pathogens. snc1 displays a stunted phenotype that may be caused by both the accumulation of toxic compounds and energy squandered on unnecessary defence instead of normal growth. The distinctive morphological phenotype of snc1 is intimately associated with the other resistance phenotypes, and provides a robust genetic tool for dissecting the signalling events downstream of snc1. To identify genes important for defence signalling, we carried out a suppressor screen to identify modifier of snc1 (mos) mutants that restore the wild type size and morphology in the snc1 background. Furthermore, in most cases, a loss of sneakiness in mos mutants correlated with a reduced or abolished constitutive PR gene expression, SA accumulation and pathogen resistance in snc1 plants. These loss of function mutants represent defects in positive regulators of the snc1 pathway. I cloned and characterized two mos mutants, and showed that they both have roles in Arabidopsis innate immunity as well. mos6 partially suppresses snc1 and exhibits enhanced disease susceptibility (EDS) to an oomycete pathogen. MOS6, identified by map-based cloning, encodes an alpha-importin subunit, one of 8 found in Arabidopsis, and has a demonstrated role in nucleocytoplasmic partitioning (protein import). Two other genes cloned by others from this screen, MOS3 and MOS7, encode components of the nuclear pore complex, implicating nuclear trafficking as a key regulator in plant innate immunity. mos4 exhibits EDS to virulent and avirulent bacterial and oomycete pathogens. There is evidence that MOS4-mediated resistance is independent of the signalling protein NPR1. MOS4 encodes a protein with homology to human Breast Cancer Amplified Sequence 2 and with predicted protein-protein interaction domains. Subcellular localization of MOS4-GFP shows that MOS4 is localized to the nucleus. To illuminate the biochemical function of MOS4, a yeast-2-hybrid screen was conducted. One MOS4-interactor was a putative myb transcription factor, MOS4-Associated Complex Protein 1 (MAC1), also known at AtCDC5. MAC1 interacts directly with MOS4 in vitro and in planta. mac1 insertional mutants exhibit defects in immune responses to pathogens similar to that of mos4. In addition, mac1 also partially suppressed snc1 morphology and enhanced resistance. Both MOS4 and MAC1 have homologs in humans and fission yeast that are members of a discrete protein complex that has been implicated in several different biological processes including RNA splicing, apoptosis and protein degradation. Using proteomics data from yeast and human, we found genes with homology to additional components of the orthologous complex in Arabidopsis, and isolated insertion mutants in these. Mutations in PRL1, which encodes a WD protein, display similar disease phenotypes to that of mos4 and mac1. AtCDC5 has DNA binding activity, suggesting that this complex may regulate defence responses through transcriptional control. Since the complex components along with their interactions are highly conserved from fission yeast to Arabidopsis and human, they may also have a yet-to-be identified function in mammalian innate immunity.

General purpose rich-text editors, such as MS Word are often used to author software requirements specifications. These requirements specifications contain many different logical structures, such as use cases, business rules and functional requirements. Automated recognition and extraction of these logical structures is necessary to provide useful automated requirements management features, such as automated traceability, template conformance checking, guided editing and interoperability with sophisticated requirements management tools like Requisite Pro. The variability among instances of these logical structures and their attributes poses many challenges for their accurate recognition and extraction. The thesis provides a framework for the extraction of logical structures from software requirements documents. The framework models information about style, structure, and attributes of the logical structures and uses the defined meta-model to extract instances of logical structures. A meta-model also incorporates information about the variability present in the instances. The framework includes an extraction tool, ET, that reads the meta-model and extracts instances of modelled logical structures from the documents. The framework is evaluated on a collection of real-world software requirements documents. Using the framework, different logical structures can be extracted with high precision and recall, each close to 100%. The performance of the extraction tool is acceptable for fast extraction of logical structures from documents with extraction times ranging from a few milliseconds to a few seconds.

Plants have evolved sophisticated defence systems against pathogen infection. Initiation of induced defence signalling often involves specific recognition of invading pathogens by the products of specialized host Resistance (R) genes. Consequently, the pathogen is stopped at the site of infection. A unique dominant mutant in Arabidopsis thaliana, snc1, constitutively expresses pathogensis-related (PR) genes and exhibits enhanced resistance to bacterial and oomycete pathogens. SNC1 encodes an R-gene – a single amino acid change renders this protein constitutively active without interaction with pathogens. snc1 displays a stunted phenotype that may be caused by both the accumulation of toxic compounds and energy squandered on unnecessary defence instead of normal growth. The distinctive morphological phenotype of snc1 is intimately associated with the other resistance phenotypes, and provides a robust genetic tool for dissecting the signalling events downstream of snc1. To identify genes important for defence signalling, we carried out a suppressor screen to identify modifier of snc1 (mos) mutants that restore the wild type size and morphology in the snc1 background. Furthermore, in most cases, a loss of sneakiness in mos mutants correlated with a reduced or abolished constitutive PR gene expression, SA accumulation and pathogen resistance in snc1 plants. These loss of function mutants represent defects in positive regulators of the snc1 pathway. I cloned and characterized two mos mutants, and showed that they both have roles in Arabidopsis innate immunity as well. mos6 partially suppresses snc1 and exhibits enhanced disease susceptibility (EDS) to an oomycete pathogen. MOS6, identified by map-based cloning, encodes an alpha-importin subunit, one of 8 found in Arabidopsis, and has a demonstrated role in nucleocytoplasmic partitioning (protein import). Two other genes cloned by others from this screen, MOS3 and MOS7, encode components of the nuclear pore complex, implicating nuclear trafficking as a key regulator in plant innate immunity. mos4 exhibits EDS to virulent and avirulent bacterial and oomycete pathogens. There is evidence that MOS4-mediated resistance is independent of the signalling protein NPR1. MOS4 encodes a protein with homology to human Breast Cancer Amplified Sequence 2 and with predicted protein-protein interaction domains. Subcellular localization of MOS4-GFP shows that MOS4 is localized to the nucleus. To illuminate the biochemical function of MOS4, a yeast-2-hybrid screen was conducted. One MOS4-interactor was a putative myb transcription factor, MOS4-Associated Complex Protein 1 (MAC1), also known at AtCDC5. MAC1 interacts directly with MOS4 in vitro and in planta. mac1 insertional mutants exhibit defects in immune responses to pathogens similar to that of mos4. In addition, mac1 also partially suppressed snc1 morphology and enhanced resistance. Both MOS4 and MAC1 have homologs in humans and fission yeast that are members of a discrete protein complex that has been implicated in several different biological processes including RNA splicing, apoptosis and protein degradation. Using proteomics data from yeast and human, we found genes with homology to additional components of the orthologous complex in Arabidopsis, and isolated insertion mutants in these. Mutations in PRL1, which encodes a WD protein, display similar disease phenotypes to that of mos4 and mac1. AtCDC5 has DNA binding activity, suggesting that this complex may regulate defence responses through transcriptional control. Since the complex components along with their interactions are highly conserved from fission yeast to Arabidopsis and human, they may also have a yet-to-be identified function in mammalian innate immunity.

No description available.

General purpose rich-text editors, such as MS Word are often used to author software requirements specifications. These requirements specifications contain many different logical structures, such as use cases, business rules and functional requirements. Automated recognition and extraction of these logical structures is necessary to provide useful automated requirements management features, such as automated traceability, template conformance checking, guided editing and interoperability with sophisticated requirements management tools like Requisite Pro. The variability among instances of these logical structures and their attributes poses many challenges for their accurate recognition and extraction. The thesis provides a framework for the extraction of logical structures from software requirements documents. The framework models information about style, structure, and attributes of the logical structures and uses the defined meta-model to extract instances of logical structures. A meta-model also incorporates information about the variability present in the instances. The framework includes an extraction tool, ET, that reads the meta-model and extracts instances of modelled logical structures from the documents. The framework is evaluated on a collection of real-world software requirements documents. Using the framework, different logical structures can be extracted with high precision and recall, each close to 100%. The performance of the extraction tool is acceptable for fast extraction of logical structures from documents with extraction times ranging from a few milliseconds to a few seconds.

Thesis (doctoral)--Universität Bern.