En este estudio se examina la creación de la idea de negocio llamada Easy Move. Esta idea de negocio quiere aprovechar el boom inmobiliaria que se presenta en Perú adaptando las necesidades del mercado al servicio. El concepto de este servicio es de conectar a un transportista independiente con un cliente interesado en el servicio de mudanza. Por medio de hipótesis y concierge se examinan los posibles escenarios de este negocio y se va dando forma a la idea negocio de acuerdo a las necesidades de los clientes. Los datos analizados muestran que existen diferentes tipos de necesidades en las personas que se quieren mudar. Se identifican diferentes tipos de clientes, las personas que planifican su mudanza, las personas que no planifican su mudanza y necesitan mudarse de inmediato y los transportistas independientes que necesitan clientes interesados en sus servicios. Además, los datos analizados reflejan que, si se resuelve el problema de un potencial cliente, este se sentirá satisfecho y adquirirá el servicio. / This study examines the creation of the business idea called Easy Move. This business idea is going to take advantage of the real estate boom that is present in Peru, adapting the needs of the market to the service. The concept of this service is to connect an independent carrier with a customer interested in moving services. By means of hypothesis and concierge, the possible scenarios of this business are examined, and the business idea is shaped according to the needs of the clients. The data analysed showed that there are different types of needs in people who want to move. Different types of clients are identified, the people who plan their move, the people who do not plan their move, and need to move immediately and the independent carriers that need clients interested in their services. In addition, the analysed data reflects that if the problem of a potential client is solved, they will feel satisfied and will require the service. / Trabajo de investigación

Molecular dynamics simulation was used to study ion permeation through different membrane proteins embedded in a lipid bilayer (DMPC) with different saline solutions. The potential of mean force (PMF) for ion transport was obtained by umbrella sampling simulations. A revised MacKerell force field for tryptophan residues was studied using gramicidin A (gA) channel as a test model. The revised force field contribution to the Na+ PMF was consonant with the prediction from the experimental results, but in stark contrast to the prediction of the CHARMM force field, version 22, for the tryptophan side-chain. A new grid-based correction map algorithm by MacKerell group, called CMAP, was introduced into the CHARMM force field, version 31. The CMAP algorithm focused on optimizing phi, psi dihedral parameters for the peptide backbone. The CMAP corrections reduced the excessive translocation barrier. Decomposition demonstrated the reduction in the translocation barrier was due to effects on the K+ PMFH2O rather than on K+ PMFgA. The presence of negatively charged sulfonate group at the entrance and exit of the gA channel affected the depth and the location of the highly occupied sites. The negatively charged sulfonate group produced a strong attraction for the cations in the bulk towards the channel mouth. In the M2 transmembrane domain channel (M2-TMD), three M2-TMD structures were studied, differing only in whether the selectivity-filter (four His37 side-chains) was uncharged, +2 charged, or +3 charged. M2-TMD structural properties were compared with the structural properties of other models extracted from NMR and X-ray studies. The spontaneous cation and anion entry into the charged selectivity-filter was different from that into a neutral selectivity-filter. Cl- ions had a lower free-energy barrier in the selectivity-filter than either Na+ or NH4+ ions through the M2-TMD channel. NH4+ ions had a lower free-energy barrier in the selectivity-filter than Na+ ions. Based on accessible rotamer conformations, a revised conductance mechanism was proposed. In this conductance mechanism, the His37 side-chain functioned as an acceptor and donor group, whereas the Trp41 side-chain functioned as a carrying group.