A comprehensive understanding of urban development is critical for moving towards the goal of sustainability. Despite a collection of urban sustainability indicator (USI) conceptual frameworks proposed and explored in practical urban sustainability assessment, establishing an integrated, well-quantified, spatially characterized USI model is still a challenging task. Therefore, based on a manuscript-style format this thesis develops a subjectively weighted integrated USI model and then applies it to the city of Saskatoon, SK, Canada, as a case study, based on quantifying a hierarchical index system. In addition, urban environmental sustainability is spatiotemporally investigated for an improved understanding of Urban Heat Island (UHI) effect. Results show that the proposed integrated USI model improved urban sustainability measurement by overcoming the shortages in existing USI models. Geospatial statistics demonstrated disparity in urban sustainability across residential neighbourhoods for Saskatoon in 2006 based on the significant clusters and outliers. It also found that population increases can possibly improve intellectual and economic well-being and promote urbanization, but may cause environmental degradation and lead to a decline in overall urban sustainability. This research also demonstrates that satellite imagery can be used to study environmental sustainability at different spatiotemporal scales. This research reveals that both urban water and green spaces had significant cooling effects on the surrounding urban LST within specific ranges. Urban surface temperature can be estimated based on a multiple linear regression model with sustainable traveling mode index and land use information as input variables. The overall significance of this research has three folds. First, it lays a preliminary theoretical foundation for a comprehensive understanding of urban sustainability based on a well-quantified integrated USI model. Second, it is relatively original with respect to improving urban sustainability measurements through the incorporation of subjective information into objective data. Third, this research has explored spatiotemporal analysis to detect urban sustainability patterns based on compiling multiple data sources using geospatial techniques. The proposed USI model is highly suitable for comparison analysis at different spatial scales as well as continuously tracking the dynamic changes. Therefore, this research can be a good practice of applying the spatiotemporal philosophy to urban geographical problems.

In this paper, Swedish wolf population management was analyzed by two models. One is information index system to measure the positive and normative information held by the stakeholders. By using this system, the quantitative information indices can be measured to give people a clear understanding of the current situation of themselves and other stakeholders in order to enhance communication and engagement. By testing the current information shortage from the national survey, it was confirmed that the information shortage do exist in the society and the need for improving the information acquirement is significant. Besides, satisfaction functions of positive and negative stakeholders towards wolf population were used to test an agreeable wolf population. Stakeholder satisfaction was expressed as a function of wolf pupulation, either positively or negatively correlated, using economic and social features such as taxes, compensation, preventative payments, lupophobia, biphilia, etc. Weights were given to derive overall goal functions for pro- and anti-wolf stakeholders in order to find if there exist wolf population levels which might indicate include common preference. Whereas, current wolf pupulation is around 210 in Sweden, the results showed that all stakeholder groups could be satisfied with a population of around 500 wolves. Some major policy measures were studied for their influence on stakeholders' interests, in particular in how to increase wolf population in order to achieve this solution.

<p>In many forensic cases, such as mass disasters reconstruction cases, the recovered DNA is highly degraded. In such incidences, typing of STR loci has become one of the most powerful tools for retrieving information from the degraded DNA. However, as DNA degradation proceeds, three phenomena occur consecutively: loci imbalance, allele dropout and no amplification. To solve the problem of degraded DNA, redesigned primer sets have been developed in which the primers were positioned as close as possible to the STR repeat region. These reduced primer sets were called Miniplexes. Unfortunately, a few of the CODIS STR loci cannot be made into smaller amplicons. For this reason non-CODIS miniSTRs have been developed. The present study was undertaken for the population genetic analysis of microsatellite variation in four South African populations / Afrikaner, Xhosa, Mixed Ancestry and Asian Indian using eight non-CODIS miniSTR loci. These miniSTRs loci were characterized within the populations by estimating the levels of diversity of the markers, estimating the population genetic parameters, and studying the inter-population relationships. All of the miniSTRs were amplified successfully and the genetic variability parameters across all loci in Afrikaner, Mixed Ancestry, Asian Indian and Xhosa were estimated to be in the range of 3 (D4S2364) to 12 (D9S2157) alleles, the total number of alleles over all loci ranged from 100 to 204, the allelic richness ranged from 3.612 to 10.307 and the heterozygosity ranged from 0.4360 to 0.8073. Genetic distance was least between Afrikaner and Asian Indian and highest between Xhosa and Mixed Ancestry. Deviations from Hardy-Weinberg equilibrium were not observed for most of the loci. The low mean FIS (-0.027) and FIT (-0.010) and FST (0.017) values across the populations indicated low level of inbreeding within (FIS) and among (FST) the populations. The Asian Indian population showed higher levels of the inbreeding coefficient, indicating less gene exchange between it and other populations. These 8 markers can be used for genetic investigations and assessing population structure. The study contributed to the knowledge and genetic characterization of four South African populations. In addition, these MiniSTRs prove to be useful in cases where more genetic information is needed.</p>

<p>In many forensic cases, such as mass disasters reconstruction cases, the recovered DNA is highly degraded. In such incidences, typing of STR loci has become one of the most powerful tools for retrieving information from the degraded DNA. However, as DNA degradation proceeds, three phenomena occur consecutively: loci imbalance, allele dropout and no amplification. To solve the problem of degraded DNA, redesigned primer sets have been developed in which the primers were positioned as close as possible to the STR repeat region. These reduced primer sets were called Miniplexes. Unfortunately, a few of the CODIS STR loci cannot be made into smaller amplicons. For this reason non-CODIS miniSTRs have been developed. The present study was undertaken for the population genetic analysis of microsatellite variation in four South African populations / Afrikaner, Xhosa, Mixed Ancestry and Asian Indian using eight non-CODIS miniSTR loci. These miniSTRs loci were characterized within the populations by estimating the levels of diversity of the markers, estimating the population genetic parameters, and studying the inter-population relationships. All of the miniSTRs were amplified successfully and the genetic variability parameters across all loci in Afrikaner, Mixed Ancestry, Asian Indian and Xhosa were estimated to be in the range of 3 (D4S2364) to 12 (D9S2157) alleles, the total number of alleles over all loci ranged from 100 to 204, the allelic richness ranged from 3.612 to 10.307 and the heterozygosity ranged from 0.4360 to 0.8073. Genetic distance was least between Afrikaner and Asian Indian and highest between Xhosa and Mixed Ancestry. Deviations from Hardy-Weinberg equilibrium were not observed for most of the loci. The low mean FIS (-0.027) and FIT (-0.010) and FST (0.017) values across the populations indicated low level of inbreeding within (FIS) and among (FST) the populations. The Asian Indian population showed higher levels of the inbreeding coefficient, indicating less gene exchange between it and other populations. These 8 markers can be used for genetic investigations and assessing population structure. The study contributed to the knowledge and genetic characterization of four South African populations. In addition, these MiniSTRs prove to be useful in cases where more genetic information is needed.</p>

Magister Scientiae - MSc / In many forensic cases, such as mass disasters reconstruction cases, the recovered DNA is highly degraded. In such incidences, typing of STR loci has become one of the most powerful tools for retrieving information from the degraded DNA. However, as DNA degradation proceeds, three phenomena occur consecutively: loci imbalance, allele dropout and no amplification. To solve the problem of degraded DNA, redesigned primer sets have been developed in which the primers were positioned as close as possible to the STR repeat region. These reduced primer sets were called Miniplexes. Unfortunately, a few of the CODIS STR loci cannot be made into smaller amplicons. For this reason non-CODIS miniSTRs have been developed. The present study was undertaken for the population genetic analysis of microsatellite variation in four South African populations; Afrikaner, Xhosa, Mixed Ancestry and Asian Indian using eight non-CODIS miniSTR loci. These miniSTRs loci were characterized within the populations by estimating the levels of diversity of the markers, estimating the population genetic parameters, and studying the inter-population relationships. All of the miniSTRs were amplified successfully and the genetic variability parameters across all loci in Afrikaner, Mixed Ancestry, Asian Indian and Xhosa were estimated to be in the range of 3 (D4S2364) to 12 (D9S2157) alleles, the total number of alleles over all loci ranged from 100 to 204, the allelic richness ranged from 3.612 to 10.307 and the heterozygosity ranged from 0.4360 to 0.8073. Genetic distance was least between Afrikaner and Asian Indian and highest between Xhosa and Mixed Ancestry. Deviations from Hardy-Weinberg equilibrium were not observed for most of the loci. The low mean FIS (-0.027) and FIT (-0.010) and FST (0.017) values across the populations indicated low level of inbreeding within (FIS) and among (FST) the populations. The Asian Indian population showed higher levels of the inbreeding coefficient, indicating less gene exchange between it and other populations. These 8 markers can be used for genetic investigations and assessing population structure. The study contributed to the knowledge and genetic characterization of four South African populations. In addition, these MiniSTRs prove to be useful in cases where more genetic information is needed. / South Africa