Orientador: Renata Fracácio Francisco / Resumo: A indústria têxtil é responsável pela maior parte de corantes utilizados comercialmente. Segundo a literatura estes contaminantes representam potencial tóxico a fauna aquática. Neste contexto destaca-se o corante sintético, Índigo Blue, por seu uso intenso no tingimento do “blue jeans”. Antes o corante índigo era obtido da planta Indigofera tinctoria, mas a viabilidade econômica sobrepôs a produção natural e tornou a cor azul acessível a sociedade com a produção sintética do corante. Este estudo objetivou comparar os aspectos ecotoxicológicos do corante Índigo Blue (sintético) e do corante Índigo Natural (obtido da planta Indigofera tinctoria) expondo-se organismos de dois níveis tróficos diferentes à 100 mg/L de cada corante (concentração representativa de corantes em efluntes têxteis). Os organismos-teste utilizados foram: 1) a alga Raphidocelis subcapitata com duração dos testes equivalente a 72h e 96h, avaliando-se o parâmetro crescimento pela contagem do numero de células em relação ao grupo controle ( a concentração estudada não inibiu o crescimento algal) e 2) o peixe Danio rerio, machos e fêmeas mantiveram-se expostos por 21 dias, num total de seis réplicas para cada tratamento e para cada sexo. Sendo 3 organismos-teste em cada uma das quatro réplicas destinadas para a histologia de gônadas de ambos os sexos e duas réplicas com 4 machos destinados para a reprodução e duas réplicas cotendo 2 fêmeas para a reprodução (proporção de dois machos pra cada fêmea). Após o vig... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: The textile industry is responsible for most commercially used dyes. According to the literature these contaminants represent toxic potential to aquatic fauna. In this context the synthetic dye, Indigo Blue, stands out due to its intense use in dyeing blue jeans. Before, indigo dye was obtained from the Indigofera tinctoria plant, but economic viability superimposed the natural production and made the blue color accessible to society with the synthetic dye production. This study aimed to compare the ecotoxicological aspects of Indigo Blue (synthetic) and Indigo Natural dye (obtained from the Indigofera tinctoria plant) by exposing organisms of two different trophic levels to 100 mg / L of each dye (representative concentrations of dyes in flakes textiles). The test organisms used were: 1) Raphidocelis subcapitata seaweed with duration of the tests equivalent to 72h and 96h, being evaluated the parameter growth by counting the number of cells in relation to the control group (the concentration studied did not inhibit algal growth) and 2) the Danio rerio fish, males and females remained exposed for 21 days, for a total of six replicates for each treatment and for each sex. Three replicates were used in each of the four replicates for the histology of gonads of both sexes and two replicates with 4 males destined for breeding and 2 replicates with 2 females for reproduction (ratio of two males to each female). After the twenty-first day, two replicates destined for histology were... (Complete abstract click electronic access below) / Mestre

An impact of nitrogen, phosphorus and potassium fertilizers on the concentration changes of carotenoid pigments in the Indigofera tinctoria plant by using Raman spectroscopic techniques is studied. Three different concentration levels of the fertilizers with a normal supply as control were added to the plants at two stages. The Raman spectra were taken to determine the carotenoid concentration level changes in the plant leaves in vivo. The ring-stretching mode are the Raman spectroscopic signatures for the carotenoid pigment and its magnitude increased significantly (over 170%) for the case of phosphorus and potassium fertilizers. The effect from the nitrogen fertilizer was detected to be about 130% in comparison with the corresponding control plants. This study has a potential application for the increased extraction of the indigotin dye from plants for the medical and textile industries.