• Refine Query
  • Source
  • Publication year
  • to
  • Language
  • 1
  • Tagged with
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Análise da calogênese de paricá por modelos de regressão logística

SOUZA, Lucas Batista de 23 July 2015 (has links)
Neste estudo concernente ao paricá (Schizolobium amazonicum), objetivou-se avaliar a interação entre diferentes fontes de explantes e a concentração de fitorreguladores sobre a indução de calos, por meio da abordagem de modelos de regressão linear generalizados. Os explantes foram extraídos de plântulas germinadas in vitro. Foram utilizados os segmentos apical, cotiledonar, nodal e de raiz para indução com 2,4-diclorofenoxiacético (2,4-D) de 0,0 a 2,1 mg L-1 e, foi ainda testado o segmento hipocotiledonar para a indução com a combinação de ácido indol-3-butírico (AIB) e 6-benzilaminopurina (BAP), ambos de 0,0 a 1,0 mg L-1. Foi utilizado o meio Woody Plant Medium (WPM) acrescido de 0,3% de sacarose, 0,1% de polivinilpirrolidone (PVP) e 0,6% de ágar. Os parâmetros avaliados foram o percentual de calos, o percentual de cobertura do explante por calos e a razão da massa seca pela massa fresca. Como resultados, obteve-se 100% de calogênese nos explantes em contato com as menores concentrações aplicadas, exceto os segmentos de raiz. Também se reporta que as maiores chances de cobertura por calos foram observadas no explante nodal, tendo-se utilizado 2,4-D, e no explante cotiledonar, ao se empregar a combinação de AIB com BAP. Os modelos ajustados selecionados se mostraram uma boa alternativa de inferência por permitirem a avaliação e estudo de variáveis independentes com distribuição diferente da normal. / In this study concerning the parica (Schizolobium amazonicum), it was aimed to evaluate the interaction among different explant sources and plant growth regulators concentration on callus induction, through the approach of generalized linear models. The explants were extracted from seedlings germinated in vitro. Apical, cotyledon, root and nodal segments were used for induction with 2,4-dichlorophenoxyacetic acid (2,4-D) from 0,0 to 2,1 mg L-1 and, was still tested hypocotyledonary segments for induction with indol-3-butyric acid (IBA) and 6-benzylaminopurine (BAP) combined from 0,0 to 1,0 mg L-1. It was used Woody Plant Medium (WPM) supplemented with 0,3% sucrose, 0,1% polyvinylpyrrolidone (PVP) and 0,6% agar. It was evaluated the callus percentage, the explant coverage percentage by callus and the ratio of dry mass by fresh mass. As a result, it was obtained 100% callus formation in that explants in contact with the lower concentrations applied, except for the root segments. It also reports that the higher chances of callus coverage were observed at the nodal explant, having been used 2,4-D, and the cotyledon explants, by using the IBA with BAP combination. The adjusted models selected proved itself a good inference alternative for allowing the evaluation and study of independent variables with distribution other than normal.

Page generated in 0.0482 seconds