Muscle proteins are in a continuous state of recycling. This process involves a balance between synthesis and breakdown. These opposing processes dictate muscle protein gains and losses. Muscle hypertrophy occurs when synthesis exceeds breakdown. In order for the accretion of new muscle proteins, a chronic state of net positive muscle protein balance (synthesis> breakdown) is required. Resistance exercise is a potent stimulus of protein turnover and the combined effects of exercise and feeding have shown to be necessary for net protein anabolism. Resistance training has been reported to increase muscle strength and induce changes in skeletal muscle morphology. These positive strength adaptations include muscle fibre hypertrophy and a shift in fibre type from IJX to IIA. Previous investigations of resistance training-induced changes in muscle protein synthesis and fibre morphology have utilized cross-sectional or longitudinal, bilateral training designs. Thus, the purpose of this study was to investigate the effects of a progressive eight week unilateral leg resistance training program on skeletal muscle morphology, and resting and exercise-stimulated mixed muscle protein fractional synthesis rate (FSR). Eight young men performed two training sessions each week, and each session consisted of four sets of knee extension (KE) and four sets of leg press (LP) at 80% 1 repetition maximum (1 RM). Needle biopsies from the vastus lateralis muscle of the trained (T) leg were taken before and after training and analyzed for fibre composition, cross-sectional area (CSA), and myosin heavy chain (MHC) content. Muscle protein FSR was determined using a primed constant stable isotope infusion of [13C6]-phenylalanine in both the T and untrained (UT) legs. Training induced type IIX and IIA fibre hypertrophy (P <0.05) with no change for 1ype I fibre CSA. There was no significant change in histochemically determined fibre composition or MHC content. After training, 1RM strength of the T leg significantly increased compared to baseline values (P < 0.01). At rest, FSR was significantly elevated in the T versus the UT leg (P < 0.01). Following an acute bout of resistance exercise, which was performed at the same relative intensity (80% 1 RM) for the T and UT legs, FSR was greater in the UT versus the T leg (P < 0.01). There was a lower exercise-induced increase in muscle FSR in the T versus the UT leg compared to their respective resting values <T: P = 0.08, UT: P < 0.01). These data show that resistance training resulted in significant muscle fibre hypertrophy and elevated rate of muscle protein synthesis at rest. In addition, the acute response to resistance exercise was characterized by an attenuated rise in muscle protein FSR in the T versus the UT leg. We conclude that resistance training markedly attenuates the acute muscle protein synthetic response following resistance exercise, even when loads are matched at the same relative intensity. / Thesis / Master of Science (MS)

Bluff bodies immersed in a fluid stream are susceptible to flow-induced vibrations. Depending on the body dynamic characteristics and flow conditions, different types of flow-induced vibrations may occur. The failure of a blade in a large mixing vessel in a chemical plant raised the question of the response of a parabolic cross-section bluff body to the flow excitation. Experiments were conducted in a wind tunnel using two- dimensional “sectional” models. Models with parabolic, semi-elliptic and semi-circular cross-section were investigated. In the dynamic experiments, flow velocity was increased from 0 to 22 m\s, and the oscillating amplitude and wake response were monitored. Vortex-induced vibrations were observed with Strouhal numbers for parabolic and semi-circular cross-sections of 0.13 and 0.12, respectively. Steady lift force and fluid moment for different angles of attack were monitored in the static experiments. From these results, lift and moment coefficients were calculated. For the closed semi-circular cross-section, Reynolds number had a strong influence on the lift coefficient. With an increase in Reynolds number, the lift coefficient decreased. The largest difference was noted at an angle of attack a = 45°. In contrast, the open semi-circular model lift coefficient was independent of Reynolds number. In the experiments where the elastic axis of the model coincided with the model centre of gravity, galloping was not observed in the plunge mode. When the model elastic axis was moved to a position 90 mm behind the test model centre of gravity, galloping was observed for the semi-elliptic and parabolic models. The onset of galloping coincided with the vortex-induced resonance. Changing the model elastic axis position introduced a combination of plunge and torsional motion, and latter is believed to be responsible for the existence of galloping. The parabolic model was modified in an attempt to eliminate galloping instability. Fins were added at the separation points to widen the wake and prevent the reattachment of the flow to the afterbody. With these changes, galloping was not observed, although the oscillation amplitudes remained unacceptably high. The present investigation revealed previously unknown characteristics of semi-elliptical and parabolic cross-section bluff body behaviour in fluid flow. At the same time, it laid a foundation for the solution to the practical problem encountered when a parabolic cross-section bluff body was used as a mixing blade. / Thesis / Master of Engineering (ME)

Some studies have demonstrated that the effects of a drug may be different, depending on whether the drug is self-administered or passively received by the subject. Most of the studies which have examined this phenomenon have not examined the effects of a drug following each of a series of administrations. Moreover, the mechanism mediating differences between self-administered and passively received drugs has not been determined. The present experiments used a yoked-control design to examine the development of tolerance to the ataxic effects of heroin and of ethanol in rats that self-administer the drugs and rats that passively received them. Results demonstrate that rats that passively received heroin, but not those that self-administered the drug, were significantly impaired following the initial administrations. During the first administration sessions, rats that self-administered ethanol were as impaired as their partners that passively received, but within a few sessions self-administering rats developed tolerance to the ataxic effect of the ethanol, while their yoked partners did not. The results also suggest that the faster tolerance development in rats that self-administered ethanol may have been mediated by differences in Pavlovian conditioning in these subjects, which demonstrated larger compensatory conditional responses in the form of “hypertaxia” than did their yoked partners. The results indicated that some component of the self-administration process contributed to the Pavlovian conditioning, and hence, faster tolerance development, of self-administering animals. The data suggest that studies in which drugs are passively received may overestimate the dose that is necessary to produce tolerance in self-administering animals. Models based on such studies, then, may require modification before they are applied to situations which involve self-administration of drugs. / Thesis / Doctor of Philosophy (PhD)

Optical diagnostics have become indispensable tools for the study of turbulent flows and flames. However, optical diagnostics developed in the past have been primarily limited to measurements at a point, along a line, or across a two-dimensional (2D) plane; while turbulent flows and flames are inherently four-dimensional (three-dimensional in space and transient in time). As a result, diagnostic techniques which can provide 4D measurement have been long desired. The purpose of this dissertation is to investigate two of such 4D diagnostics both for the fundamental study of turbulent flow and combustion processes and also for the applied research of practical devices. These two diagnostics are respectively code named tomographic chemiluminescence (TC) and volumetric laser induced fluorescence (VLIF). For the TC technique, the emission of light as the result of combustion (i.e. chemiluminescence) is firstly recorded by multiple cameras placed at different orientations. A numerical algorithm is then applied on the data recorded to reconstruct the 4D flame structure. For the VLIF technique, a laser is used to excite a specific species in the flow or flame. The excited species then de-excite to emit light at a wavelength longer than the laser wavelength. The emitted light is then captured by optical sensors and again, the numerical algorithm is applied to reconstruct the flow or flame structure. This dissertation describes the numerical and experimental validation of these two techniques, and explores their capabilities and limitations. It is expected that the results obtained in this dissertation lay the groundwork for further development and expanded application of 4D diagnostics for the study of turbulent flows and combustion processes. / Ph. D.

In vitro data from our laboratory demonstrate that vitamin B6 (B6) supplementation of estrogen receptor - positive and - negative breast cancer cells is growth inhibitory. Others have reported that dietary B6 supplementation resulted in increased fibrosarcoma pyridoxal phosphate (PLP) concentrations and a significant inverse relationship between tumor PLP concentration and tumor volume in mice. This suggests that, in contrast to data reported for normal cells, tumor cells are capable of accumulating supplemental B6. In the current study, we investigated the effects of dietary B6 supplementation on 7,12-dimethylbenz(a)anthracene (DMBA)-induced mammary carcinoma in rats. Specifically, we aimed to identify the effect of pyridoxine (PN) supplementation on tumor growth and vitamin uptake by tumor cells. To accomplish this, 50 d old female Sprague Dawley rats were gavaged with 15 mg DMBA and fed a diet containing either 7, 350, or 1050 mg PN-HCl/kg diet, which is the equivalent of 1, 50, or 150x the National Research Council's B6 requirement for rats, respectively. These levels of PN have previously been shown to produce no overt signs of toxicity in rats. Throughout the experiment, the percent of rats with tumors and the average number of tumors per rat remained similar between groups. Mammary tumor growth rates were significantly increased in response to dietary B6 supplementation (P < 0.05). Liver PLP and pyridoxal (PL) concentrations did not differ between dietary treatment groups. Plasma PL and PLP concentrations were significantly higher in the group fed the 150x diet compared with the 1x diet (P < 0.001, P < 0.05). Mammary tissue PL concentrations of the 150x group were significantly higher (P < 0.05) than the 1x group, but no differences were observed in mammary PLP concentrations. Similarly to mammary tissue, no differences between groups were observed in tumor PLP concentration. However, tumor PL concentrations in both the 50x and 150x dietary treatment groups were significantly higher than those from the rats fed the 1x diet (P < 0.002). These data demonstrate that previously reported inhibitory effects of supplemental B6 on breast cancer growth in vitro do not occur in response to dietary supplementation at 50 or 150 times the B6 requirement in vivo. In fact, dietary B6 at 150x the requirement may actually promote mammary tumor growth. In light of these results, investigation of the effects of supplemental B6 on cancer growth in humans is warranted. Supported by American Cancer Society Grant # IRG-99-225-01. / Master of Science

The wake of a bluff body is a representative issue in vortex dynamics that plays a central role in civil engineering, ocean engineering and thermal engineering. In this work, a flowing soap film was used to investigate the wakes of multiple stationary circular cylinders and of a single oscillating cylinder. Corresponding computer simulations were also conducted. Vortex formation of a stationary circular cylinder was analyzed by proper orthogonal decomposition (POD). The POD analysis was used to define an unsteady vortex formation length, which suggests a relationship between the vortex formation length of a single cylinder and the critical spacing of two cylinders in a tandem arrangement. A systematic parametric study of the wake structure was conducted for a controlled transversely oscillating cylinder. Neural network and support vector machine codes assisted the wake classification procedure and the identification of boundaries between different wake regimes. The phase map of the vortex shedding regimes for the (quasi) two-dimensional experiment qualitatively agrees with previous three-dimensional experiments. The critical spacings of two identical tandem circular cylinders in a flowing soap film system were determined using visual inspections of the wake patterns and calculations of the Strouhal frequencies. The dimensionless spacing was both increased and decreased quasi-statically. Hysteresis was observed in the flow patterns and Strouhal numbers. This study appears to provide the first experimental evidence of critical spacing values that agree with published computational results. The wake interaction between a stationary upstream circular disk and a free downstream circular disk was also investigated. With the ability to tie together the wake structure and the object motion, the relationship between energy generation and flow structure in the simplified reduced order model system was studied. The research results find the optimal efficiency of the energy harvesting system by a parametric study. / PHD / The wake of a bluff body is a classic issue in vortex dynamics that has been the subject of much research in civil engineering, ocean engineering and thermal engineering. Bluff bodies, especially circular cylinders, can be found extensively in heat exchangers, cooling systems and offshore structures. Flow-induced vibration of a bluff body due to the formation of a wake is an important problem in many fields of engineering. Flow-induced vibration determines the oscillation of flexible pipes that transfer oil from the seabed to the surface of the ocean, for example [71]. In civil engineering, flow-induced vibration affects the design of bluff structures in wind such as bridges, chimneys and buildings [62]. Flow-induced vibration caused by vortices being shed from a bluff body is also a promising way to extract energy from geophysical flows [10]. FIV energy harvesting systems are especially suitable for slow flow speeds in the range 0.5-1.5m/s which cannot be efficiently harvested by traditional hydroelectric power technologies. When a pair of tandem cylinders is immersed in a flow, the downstream cylinder can be excited into wake-induced vibrations (WIV) due to the interaction with vortices coming from the upstream cylinder. In this work, a flowing soap film was used to investigate the flow-induced vibration of the downstream cylinder of a tandem pair. With the ability to tie together the wake structure and the object motion, we investigate the relationship between energy generation and flow structure in the reduced order model system. The research results find the optimal efficiency of the energy harvesting system by a parametric study. To get deep physical understanding of the flow-induced vibration, wake structures of a circular cylinder undergoing controlled motion and the critical spacing of two identical tandem circular cylinders were also investigated in this research. These research results can help not only the optimization of energy harvesting systems based on flow-induced vibration of the circular-cylinder system, but also will benefit the understanding of wake interactions between multiple bluff bodies such as schooling fish, natural draft cooling towers and wind turbine farms.

A recent concern is the cause and effect of global climate change. Many institutions give credit for these changes to the increased levels of greenhouse gases in the atmosphere, in particular the increase in the amount of carbon dioxide present. There is a growing interest in carbon capture and storage (CCS) as a means to reduce the global impact of CO₂ on the climate as a greenhouse gas. Carbon capture is the process of removing CO₂ from the atmosphere as well as preventing it from entering the atmosphere by means of exhaust. The captured carbon is stored underground in reservoirs. These reservoirs have the storage space to handle the volume of CO₂ injected as well as a caprock layer preventing the injection fluid from returning to the surface. Additionally, CO₂ can be used for enhanced oil recovery (EOR). To monitor the injection sites used for the CO₂ storage or EOR process, the integrity of the caprock as well as the surrounding rock formations are the locations of interest. Knowing when a joint or a fracture is going to slip is necessary to prevent major failures within geologic strata. It is necessary to prevent these slips from occurring to retain the integrity of the caprock, which is keeping the fluid within the reservoirs. Passive acoustic emissions monitoring was used to determine how effectively failure locations could be located in three unique tests. Coupled with double difference tomography, the failure of a Berea Sandstone sample and Marcellus Shale sample were calculated to determine how well the stress redistribution within the sample could be mapped using the recorded data. For the main indenter tests two samples were tested, a piece of Berea Sandstone and a piece of Marcellus Shale. The secondary test was a transform shear test using sandstone, and the third test for caprock upheaval test attempted to recreate the failure of caprock due to injection pressure. For all tests, the samples were monitored using acoustic emissions software until failure or it was deduced that the test would not produce failure. The secondary tests did not progress through the data analysis as far as the indentation tests, however valuable information was gathered from these tests. The shear test demonstrated the effectiveness of the passive acoustic emissions monitoring system to record shear failure. This test provides confidence in this technology to record and located events that are not occurring in compression. The caprock upheaval tests were not successful in causing failure in the caprock, however during the testing the passive acoustic emissions monitoring system was able record and locate events that occurred within the sample around the boundary on the reservoir. At the reservoir boundaries there was evidence of fluid flowing through the reservoir, and the events align with these locations. This positive result shows that the monitoring system is able to locate events induced by fluid injection. The results of these tests provide confidence in the passive acoustic emissions monitoring system to record accurate data for the caprock integrity monitoring. The tomograms created from the recorded data accurately imaged the areas of interest within the rock samples. From these results, passive acoustic emissions monitoring systems coupled with double difference tomography has proven capable of monitoring homogeneous samples within a laboratory environment. With further testing, this technology could possibly be a viable option for monitoring carbon sequestration sites. / Master of Science

Due to their scientific significance and practical applications, turbulent flows and flames have been under extensive and intensive research for a long time. Turbulent flows and flames of interests to practice inherently have three-dimensional (3D) spatial structures, and therefore diagnostic techniques that can instantaneously resolve their 3D spatial features have long been desired and probably are needed to ultimately answer some of the open research questions. The goal of this dissertation thus is to investigate such diagnostics and demonstrate their capability and limitations in a range of turbulent flows/flames. To accomplish this goal, this dissertation developed and evaluated the following three diagnostic methods: tomographic chemiluminescence (TC), volumetric laser induced fluorescence (VLIF), and super-resolution planar laser induced fluorescence (SR-PLIF). First, 3D flame topography of well-controlled laboratory flames was measured with TC method and validated by a simultaneous 2D Mie scattering measurement. The results showed that the flame topography obtained from TC and the Mie scattering agreed qualitatively, but quantitative difference on the order of millimeter was observed between these two methods. Such difference was caused by the limitations of the TC method. The first limitation involves TC's reliance on chemiluminescence of nascent radicals (mainly CH*) in reacting flows, causing ambiguity in the definition of flame front and limiting its applications to certain types of reactive flow only. The second limitation involves TC's inability to study an isolated region of interest because the chemiluminescence is emitted everywhere in the flame. Based on the above understanding of the TC technique, the second part of this dissertation studied a VLIF method to overcome the above limitations of the TC technique. Compared with the TC technique, the VLIF method can be used in either reacting or non-reacting flow and on any particular region of interest. In the VLIF technique, the fluorescence signal was generated by exciting a target species with a laser slab of certain thickness. The signal was recorded by cameras from different perspectives, and then a VLIF tomographic algorithm was applied to resolve the spatial distribution of the concentration of the target species. An innovative 3D VLIF algorithm was proposed and validated by well-designed experiment. This model enables analysis of VLIF performance in terms of signal level, size of the field of view in 3D, and accuracy. However, due to the limited number of views and the tomographic reconstruction itself, the spatial resolution of VLIF methods is limited. Hence, the third part of this dissertation investigated a SR-PLIF method to provide a strategy to improve the spatial resolution in two spatial directions, and also to extend the measurement range of scanning 3D imaging strategies. The SR-PLIF method used planar images captured simultaneously from two (or more) orientations to reconstruct a final image with resolution enhanced or blurring removed. Both the development of SR algorithm, and the experimental demonstration of the SR-PLIF method were reported. / Ph. D. / Optical diagnostics have become indispensable tools for the study of the turbulent flows and flames. Due to the inherently 3D structure of turbulent flows and flames, diagnostic techniques which can provide 3D measurements have been long desired. Therefore, this dissertation reports the development of three optics diagnostic methods that can provide such measurement capability, with a detailed discussion of their capabilities and limitations. The methods studied are tomographic chemiluminescence (TC), volumetric laser-induced fluorescence (VLIF), and super-resolution planar laser induced fluorescence (SR-PLIF). For the TC technique, the emission of light from combustion radicals (CH* and OH*) was recorded by multiple cameras placed at different orientations. A numerical algorithm was then applied to reconstruct the 3D flame structure. For the VLIF technique, a laser slab was used to excite a specific chemical species in the flame, which were captured from different perspectives to reconstruct the flow or flame structure in 3D. For the SR-PLIF technique, a series of planar images were recorded from multiple orientations to reconstruct a target image with higher resolution or to extend the measurement volume of scanning 3D diagnostics. It is expected that the results obtained in this dissertation lay the groundwork for further development and expanded application of 3D diagnostics for the study of turbulent flows and combustion processes.

No / Myocardial infarction (MI) is an acute life-threatening disease with a high incidence worldwide. Aim of this study was to test lectin-carbohydrate binding-induced red blood cell (RBC) agglutination as an innovative tool for fast, precise and cost effective diagnosis of MI. Five lectins (Ricinus communis agglutinin (RCA), Phaseolus vulgaris erythroagglutinin (PHA), Datura stramonium agglutinin (DSA), Artocarpus agglutinin (ArA), Triticum agglutinin (TA)) were tested for ability to differentiate between agglutination characteristics in patients with MI (n = 101) or angina pectoris without MI (AP) (n = 34) and healthy volunteers (HV) as control (n =68) . RBC agglutination was analyzed by light absorbance of a stirred RBC suspension in the green to red light spectrum in an agglutimeter (amtec, Leipzig, Germany) for 15 min after lectin addition. Mean cell count in aggregates was estimated from light absorbance by a mathematical model. Each lectin induced RBC agglutination. RCA led to the strongest RBC agglutination (~500 RBCs/aggregate), while the others induced substantially slower agglutination and lead to smaller aggregate sizes (5-150 RBCs/aggregate). For all analyzed lectins the lectin-induced RBC agglutination of MI or AP patients was generally higher than for HV. However, only PHA induced agglutination that clearly distinguished MI from HV. Variance analysis showed that aggregate size after 15 min. agglutination induced by PHA was significantly higher in the MI group (143 RBCs/ aggregate) than in the HV (29 RBC-s/aggregate, p = 0.000). We hypothesize that pathological changes during MI induce modification of the carbohydrate composition on the RBC membrane and thus modify RBC agglutination. Occurrence of carbohydrate-lectin binding sites on RBC membranes provides evidence about MI. Due to significant difference in the rate of agglutination between MI > HV the differentiation between these groups is possible based on PHA-induced RBC-agglutination. This novel assay could serve as a rapid, cost effective valuable new tool for diagnosis of MI.

No / We demonstrate that nanofiltration of aqueous glycine solutions has a pronounced effect on laser-induced nucleation. Two nucleation regimes were observed in nonfiltered, irradiated solutions under isothermal conditions: a rapid initial regime associated with laser-induced nucleation and a second much slower spontaneous nucleation regime. Filtration of the solutions prior to irradiation greatly suppressed the rapid regime, while the slow regime was similar regardless of filtration or irradiation, for all supersaturations studied. A clear effect of filtration on crystal polymorphism was also observed. Nonfiltered irradiated solutions at a lower supersaturation almost exclusively yielded the α-polymorph, while at higher supersaturations there was significant presence (∼40%) of the γ-polymorph. On the other hand, filtered solutions almost exclusively yielded the α-polymorph of glycine at all supersaturations studied. These surprising results challenge some established ideas about laser-induced nucleation, showing that previously reported laser-induced nucleation phenomena in glycine aqueous solutions can be effectively suppressed by filtration, so that the underlying mechanism is unlikely to be based on molecular scale interactions involving just the solute and the solvent alone. Instead, laser-induced nucleation in this system appears to be related to either colloidal scale solution clusters or foreign solid or molecular impurities that can be removed by nanofiltration.