The study aims to find key factors of information system success in a non-profit education and training institute. The institute under study has five centers providing on-job training and long-term cultivation in the area of information technology. Facing the pressure of continuing growth and increasing competition, the institute has begun the e-projects since many years ago. After several years of system development and implementation, a survey of users¡¦ satisfaction showed that only part of the systems was acceptable. This study adopts the Technology Acceptance Model (TAM) as the primary research framework along with the service quality model and comes up with five success factors including perceived usefulness, perceived ease-of-use, system quality, information quality and service quality. Moreover, user satisfaction is taken as dependent variable of the framework. In order to differentiate the successful system from the unsuccessful system, a curriculum management system and a knowledge management system was selected to represent the successful and unsuccessful systems respectively. The case study method and questionnaire survey wre both used as research method. In the case study, the development and implementation background, process and results were investigated. The survey method was used to verify the differences between the two systems using the five factors mentioned previously. The results of questionnaire survey and statistical test clearly distinguish the two systems and support case study findings. The results of this study can serve as guideline to improve the users¡¦ satisfaction as well as to improve the information system success.