Studies were conducted to follow seasonal susceptibility of feral pink bollworm (PBW), Pectinophora gossypiella (Saunders) larvae to NuCOTN 33B (Bt) and Deltapine (DPL) 5414 in furrow and furrow plus supplementary drip-irrigated cotton field plots. Laboratory bioassays of laboratory - reared PBW larvae to flower buds and bolls and cabbage looper (CL), Trichoplusia ni (Hübner), larval mortality feeding on DPL 5415 and Bt cottons leaves were also conducted. Cry1Ac insect toxic protein contents in the different plant tissue were determined by Enzyme Linked ImmunoSorbent Assay (ELISA) throughout the season to compare in relation to PBW and CL mortality data. Irrigation type had no effect on PBW or CL larval mortality parameters measured. DPL 5415 bolls had 0.15 feral live larvae per boll and no dead larvae per boll compared with no live and 0.12 dead feral larvae per Bt boll. Whole plant samples showed 0.5 to 8.6% live larvae boll infestations compared to no live PBW life stages and no exit holes for Bt bolls. No PBW larvae survived on day four following bioassay infestation of one-third grown Bt flower buds with PBW neonate larvae as compared to 90% larval survival on DPL 5415 flower buds. Immature bolls harvested in the field and artificially infested with PBW larvae in the laboratory showed averages of 3 to 52% live larvae per boll, all in fourth instar of development, for DPL 5415 bolls compared to no live larvae, no development beyond the first instar, and no exit holes for Bt bolls. Cry1Ac protein level in flower buds were 0.11 to 0.16 ppm and 0.14, 0.11 and 0.05 ppm, in each case, per wet weight gram of boll tissue in bolls during the season. For CL leaf bioassays, larval mortalities after 7 days feeding on Bt leaves were variable ranging from 82 to 94% from node 8 on 61 and 82 days after planting (DAP) to 32, 38 and 7% on leaves from node 16 on 82, 117, and 159 DAP, respectively, and 28 and 6% on leaves from node 24 on 117 and 159 DAP. Cry1Ac amounts were 0.96 and 0.85 ppm (wet wgt per g of Bt leaf tissue), from leaves from node 8 (61 and 82 DAP), 0.53, 0.50 and 0.22 ppm (node 16, 82, 117, and 159 DAP) and 0.44 and 0.18 ppm (node 24, 117 and 159 DAP). Numbers of cotton bolls, lint and seed per acre were significantly greater from plots that were furrow plus drip irrigated as compared to furrow irrigated alone. DPL 5415 and Bt cotton yields were not significantly different.

Cotton lint contamination from honeydew excreted by sweetpotato whiteflies, Bemisia tabaci (Gennadius), is a serious problem in the textile industry resulting in reduced lint processing efficiency. The insect growth regulators, Applaud® and Knack®, provide effective control of sweetpotato whiteflies on cotton by interfering with their reproduction and development. Protection from honeydew lint contamination is attributed to reduced sweetpotato whitefly populations. We investigated the potential direct effect of Applaud and Knack on sweetpotato whitefly honeydew production. In the field, amounts of the major sugar components of honeydew produced by adults and nymphs collected on day six following Applaud or Knack applications to cotton field plots were not significantly different compared to amounts produced by those collected from untreated plots. In the laboratory, adult mortality and amounts of honeydew sugars produced by adults were not affected by confinement for 48 h on Applaud or Knack residues from cotton leaf dips or following nebulizer contact spray applications. In contrast, mortality of first and second instar nymphs on leaves was higher on day six following leaf dips in Applaud solutions compared with leaf dips in Knack or water solutions. Nymph mortality on day six following leaf dips in Knack solutions was higher than mortality of nymphs following leaf dips in water. Honeydew collected during the period between two to 50 h after leaf dip treatment had reduced amounts of glucose, fructose and trehalulose, but not sucrose and melezitose per nymph compared with honeydew from nymphs on leaves dipped in water. Results were more variable for sugars in honeydew collected 96 to 144 h after leaf dip treatment. Nebulizer sprays of Applaud and Knack to nymphs on cotton leaves also resulted in reduced amounts of sugars in honeydew and nymph mortality following treatments.

Tobacco budworm (TBW), Heliothis virescens (F.), larvae were highly susceptible to feeding on Bt cotton leaves or flower buds with 100% and 96% mortality occurring within 4 days, respectively, compared to an average mortality of 95% for cabbage looper (CL), Trichoplusia ni (Hübner), and 57% for beet armyworm (BAW), Spodoptera exigua (Hübner), after 14 days feeding on Bt leaves. Larval weights, of CL and BAW after 7, 10, or 14 days of feeding on Bt leaves were lower compared with those feeding on non-Bt cotton leaves. BAW, CL, and TBW larvae consumed significantly less Bt leaf area per feeding day compared with DPL 5415.

Season-long protection from pink bollworm (PBW), Pectinophora gossypiella (Saunders), damage has been outstanding each year, since 1996, in NuCOTN 33B7 (Bt) commercial cotton plantings in Arizona. Cotton is a perennial plant and whether or not the insect toxic protein was expressed in overwintered Bt cotton was unknown. This could be a consideration in Arizona Bt resistance monitoring in the field since occasional overwintered volunteer cotton plants may occur. In 2002 we studied Cry1Ac levels in overwintered volunteer Bt cotton plants and determined their effects on PBW and tobacco budworm (TBW), Heliothis virescens F., larval mortalities. No TBW larvae survived three-day feeding periods on Bt leaves compared with < 3% TBW larval mortality feeding on DPL 5415 leaves. PBW larval mortality after three-day feeding on flower buds or seven-day feeding on Bt cotton bolls exceeded 98% compared with 40 to 41 % mortality feeding in DPL 5415 fruiting forms. Cry1Ac toxic protein in leaves, flower buds or cotton bolls of overwintered Bt cotton was not significantly different compared with 2002 seeded cotton.

The focus of our 2002 field study was to answer a fundamental question in insect control. Once spraying has begun for a particular pest, when should it stop? In this case, we are faced with the question of when to discontinue sprays for Lygus hesperus in cotton. Cotton is susceptible to Lygus any time there are productive squares on the plant. This study developed a series of worst-case scenarios in which to provide information on timing of the latest possible sprays of economic benefit. By late planting (30 May) varieties from three different maturity groups, we were able to examine Lygus control dynamics just prior to, at, and after cutout — initiation of cut-out was defined as NAWF = 5. We found large differences in yield among the four Lygus chemical termination (LT) treatments. The earliest termination (LT1, 2 weeks prior to cut-out) suffered the largest losses to Lygus, ca. 20–50% of the maximum yield. Conversely, extending Lygus chemical control 1–3 weeks after cut-out (LT3 & LT4) provided no yield benefit whatsoever, regardless of the variety examined. Maximum yields and maximum profits were gained in the LT2, where Lygus controls were continued up to 1 week prior to cutout. Given that there was only 1 week separating the LT1 and LT2 timings, it is clear that timing is absolutely critical. The timing used in this study corresponds with previously established threshold guidelines; treat when there are at least 15 total Lygus with at least 4 nymphs per 100 sweeps. Levels far exceeded this threshold late in the season, yet additional chemical controls after cut-out provided no additional yield or control benefits. Further, we have confirmed that nymphs are the life stage of major concern with, by far, the most capacity to reduce yields. Nymphal reductions were well-correlated with yield enhancement. The best timing (LT2) achieved ca. 93% reduction in nymphal densities during the critical 3- week period around cut-out. In contrast, adult numbers were reduced by only 16% during the same period. These results establish an upper bound for treatment of Lygus, no later than 1 week prior to cut-out; however, more work is necessary to identify if earlier cessation is possible under more normal planting conditions.

We summarize information on the performance of Bt cotton against pink bollworm (PBW), Pectinophora gossypiella, in Arizona following six years of use of this new technology. Monitoring of PBW susceptibility to Bt toxin Cry1Ac was conducted annually since 1997 by collecting insects from ten to 17 cotton fields, culturing strains in the laboratory, and measuring susceptibility to Cry1Ac in diet-incorporation bioassays. Based on survival in discriminating concentration bioassays of 10 μg Cry1Ac/ml of insect diet, resistant PBW were detected in low frequencies at 10 out of 17 Arizona locations sampled in 2001 and ranged from 0.0 to 4.0%. Though significantly more abundant than in the previous three seasons, resistant PBW were statistically less abundant in 2001 than they were in 1997. One collection from Paloma, AZ, had 4.0% survivors (uncorrected, actual survival) in bioassays of 10 μg/ml and samples from Coolidge, Maricopa, and Parker Arizona yielded • 1.0% survivors of this concentration. Susceptibility of a limited numbers of 2001 collections of PBW from California, New Mexico and Texas is also reported. Bioassays of 2002 collections are underway at the time of this writing. In a parallel effort, field efficacy of Bt cotton against PBW was documented at five to 39 Arizona locations per year since 1995 by collecting cotton bolls at seasons’ end and counting PBW and exit holes. In 39 pairs of adjacent Bt and non-Bt fields evaluated in 2001 by the Arizona Cotton Research and Protection Council, mean end-of-season pink bollworm infestation levels were > 15% for non-Bt fields and were < 0.15% in adjacent Bt fields. Thus, field observations indicated that performance of Bt cotton continued to be excellent throughout Arizona in the 2002 season.

Whiteflies and Lygus bugs continue to be key pests of Arizona cotton. Some of our most popular and time-tested chemicals are still providing efficacy toward Lygus or whiteflies when used in a timely manner. However, promising new chemicals may also become available in the near future. Through research, growers can be kept updated on options for successful IPM. An experiment was conducted in order to expand our knowledge of currently available compounds and upcoming advances in insecticide development. In this experiment, 11 different compounds were tested for efficacy and duration of activity against whiteflies, Lygus, or both. Although none were active on Lygus adults, some chemicals were very effective on all stages of nymphs. Orthene® or Vydate® continue to show good results against Lygus but did not yield as high as one new compound. The best performing insecticide against Lygus was flonicamid, a novel chemistry under development by FMC. This insecticide had the best control over Lygus nymphs, was the highest yielding treatment, and required one less spray than other top performing compounds. Among newer chemistries for Lygus control is fipronil (Regent® by BASF), which performed slightly better than Vydate but not quite as effective as Orthene. Another higher-yielding regime included the use of novaluron, a novel insect growth regulator (IGR) scheduled for registration in 2005 (Diamond® by Crompton Corporation). This IGR was tested against whiteflies and Lygus bugs, but in light of yield data, Lygus efficacy should be examined more closely. None of the neonicotinoids were effective against Lygus, but several proved to be promising for whitefly control. Of the neonicotinoids tested and sprayed on threshold, dinotefuran (under development by Valent) showed good activity. The performance of spiromesifin (Oberon®, a new chemistry by Bayer) was similar to dinotefuran but needing one less spray. Intruder® out-performed all whitefly treatments, requiring only two sprays to control whiteflies season-long. Both Intruder or currently used IGRs (Knack® and Courier®) proved to be very effective against whiteflies. All insecticides in this test underwent very rigorous testing under extreme Lygus and whitefly pressures.

The integrated control concept emphasizes the importance of both chemical and biological control for pest suppression in an agricultural system. A two-year field study was conducted to evaluate the selectivity of acetamiprid for controlling sweetpotato whitefly, Bemisia tabaci, in cotton compared with a proven selective regime based on insect growth regulators (IGRs) of pyriproxyfen and buprofezin. Acetamiprid was highly effective in controlling all stages of B. tabaci compared to our untreated control and generally produced lower pest densities than the IGR regime. However, six of 14 taxa of arthropod predator were significantly depressed with the use of acetamiprid compared to our untreated control, including common species such as Geocoris punctipes, Orius tristicolor, Chrysoperla carnea, Collops vittatus, Hippodamia convergens, and Drapetis nr. divergens. Compared to other independent and concurrent studies using mixtures of broad-spectrum insecticides at the same research site, acetamiprid depressed fewer populations of predator taxa in our study, but for those taxa affected, reductions from acetamiprid were larger in many cases. In contrast, only one species was significantly reduced in the IGR regime compared with the untreated control. Predator:prey ratios were generally depressed with the use of acetamiprid compared with both the IGR and untreated control regimes. Parasitism by aphelinid parasitoids was unaffected or depressed slightly in all insecticide regimes compared with the control. Although highly efficacious for whitefly control, our results suggest that acetamiprid is a poor substitute for IGRs currently used in an integrated control program for B. tabaci in cotton.

Lygus hesperus (Knight) has been the number one pest of Arizona cotton since 1998. With advances in the selective management of Arizona cotton’s other two key pests (i.e., Bemisia tabaci and Pectinophora gossypiella), there has been less opportunity for collateral control of this yield-limiting pest. There has also been a new premium placed on locating less disruptive, even selective, control methods that are compatible with existing selective technologies. Our laboratory routinely screens candidate compounds for efficacy against Lygus hesperus under desert conditions. Promising leads are further developed and evaluated in the field for efficacy, spectrum of activity and safety for beneficial arthropods. Our recent findings have identified three compounds with potential for delivering economic control of Lygus hesperus with greater safety for beneficial arthropods than current standards of control. At the same time, our work has importantly identified many compounds that are ineffective against our Lygus, despite reported success against a related species, the tarnished plant bug, Lygus lineolaris (e.g., acetamiprid, imidacloprid, indoxacarb, pyrethroids, thiamethoxam). Flonicamid, a pyridine carboxamide, is under development by FMC in the U.S. This aphicide has shown excellent results in controlling our Lygus in cotton. Novaluron, a chitin inhibitor under development by Uniroyal (as Diamond®) in the U.S., has rate sensitive activity against Lygus in cotton. Metaflumizone (BAS320I), under development by BASF in the U.S., is a semi-carbazone chemistry with significant efficacy against Lygus hesperus. These three or potentially other new leads in insecticide discovery may play an important role in transitioning Arizona cotton growers away from neuro-toxic, broad-spectrum, and disruptive organophosphates and carbamates currently used to control Lygus in cotton. The potential benefits to natural enemy conservation should help stabilize insect pest management in Arizona cotton, thus minimizing the chances of secondary pest outbreaks and costly pest resurgences. Until selective alternatives are found and registered, acephate (e.g., Orthene 97 by Valent USA) and oxamyl (Vydate C-LV by DuPont) remain our standard recommendations for Lygus control in Arizona cotton.

When a product performs better or worse than expectations, there are many biological, ecological, and operational factors that must be examined. Genetic resistance to the pesticide itself is often a concern. The control interval depends on the ecological impact of biotic (e.g., presence and function of natural enemies) and abiotic (e.g., frequency and severity of storms) factors. Timing, rates, and application methods used are also key factors affecting product performance. A four-year study to evaluate pyriproxyfen (Knack®) field performance in Arizona was initiated in 2004, after levels of whitefly susceptibility in statewide surveys were observed to be decreasing. Grower sites in Maricopa, Buckeye, Wellton, and Marana were used. We controlled for major operational factors by using a common timing, rate, and aerial application for each Knack spray. Resistance bio-assays were conducted on progeny of field-collected adults, pre- and post-spray. New eggs were marked in-field prior to spraying and examined in the field and lab in order to isolate Knack-associated mortality caused by direct toxicity as well as by ecological factors (e.g., bioresidual). Nymphal bioassays were used to evaluate metamorphosis inhibition. Population trends were estimated using standard sampling methods. Appropriate check plots were compared to the Knack treatment. Study results suggest Knack field performance and pyriproxyfen resistance has not changed significantly among the years or locations examined to date. In 2005, many struggled to gain control over whitefly populations. This work indicated that Knack performance and resistance parameters were within the range expected for the last several years. However, operational and ecological barriers to the performance of Knack and other chemistry were in play. Late planted conditions, lush winter vegetation capable of hosting whiteflies, poor growing conditions, and an extended period of extreme immigration pressure were all factors that diminished the impact of Knack and other products in 2005. In contrast, the winter preceding 2006 was among the driest on record followed by a very active monsoon season in central Arizona. High winds and dust movement, and a very active natural enemy community helped to continually lower whitefly populations. The result was a whitefly season characterized as light, with overall foliar insecticide usage setting a 28-yr record low for Arizona cotton. Barring all other operational and ecological factors at work, control intervals should have been similar each year. Yet, observed intervals have been different (e.g., 2005 vs. 2006) and point to the importance of these external factors in assessing product performance. Work will continue in 2007 to identify factors that contribute to whitefly outbreak conditions. These data will be key to understanding any performance changes, either due to operational or ecological factors mentioned above or due to innate changes in whitefly susceptibility. This will be important in advising growers about the risk factors associated with whitefly outbreaks and should lead to recommendations for minimizing these risks.