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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

An Unexplored Genome Insulating Mechanism in Caenorhabditis Elegans

Alkhaldi, Faisal 11 1900 (has links)
Caenorhabditis Elegans genome maintains active H3K36me3 chromatin domains interspersed with repressive H3K27me3 domains on the autosomes’ distal ends. The mechanisms stabilizing these domains and the prevention of position-effect variegation remains unknown as no insulator elements have been identified in C. elegans. De-novo motif discovery applied on mes-4 binding sites links the H3K36me3-specific methyltransferase to a class of non-coding DNA known as Periodic An/Tn Clusters (PATCs). PATCs display characteristics of insulator elements such as local nucleosome depletion and their restriction to genes with specific expression profiles and chromatin marks. Finally, I describe a set of experiments to further investigate the role of PATCs and mes-4 in the maintenance of stable chromatin domains using a synthetic biology approach.

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