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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Inter and intra-laboratory variability of CD4 : a pragmatic analysis

Mlawanda, Ganizani 29 July 2011 (has links)
Background: Measuring CD4 levels is the key laboratory investigation for decision making when initiating HAART, a tertiary prevention measure to reduce HIV/AIDS mortality and morbidity. Inherent biological and analytical variability is common during CD4 enumeration. We cannot control biological variation but how significant is analytical variation to clinical decision making. Objectives: To quantify inter and intra laboratories analytical variation of CD4 counts and percentages and to determine the degree to which time lapse after sample collection contributes to the analytical coefficient of variation (CV%).To estimate the extent of disease misclassification due to CD4 variability if CD4 < 350cells/mm3. Setting: This study was conducted at the HIV clinics of RSSC Hospital, a sugar-cane estate health institution located on the north-east of Swaziland, in Lubombo district, the worst affected by HIV/AIDS in Swaziland. The laboratories involved were Lancet, Good Shepherd (GSH) and National Reference (NRL) laboratories. Study design and method: An analytical diagnostic, cross-sectional (observational) study was used in this study. Using a convenience sampling technique and after obtaining consent from participants, blood was collected in EDTA tubes and sub-divided into three samples, each for Lancet, GSH and NRL. The samples were further split into two at each respective laboratory, one of which was run at 12hours and the other at 24hours from the time of sample collection. Main outcome measures: Student t-test; analytical coefficient of variation (CV%); Bland and Altman (BA) method bias and limits of confidence; BA plots and percentage difference plots; concordance correlation, Pearson and Kappa coefficients; McNemar test for comparison of paired proportion. Results: Fifty three participants consented for participation and of these twenty eight participants were male. The mean CD4 was 373.4 cells/mm3 for Lancet, 395.9 cells/mm3 for NRL and 439.2 cells/mm3 for GSH and subsequent paired t-test revealed some inherent variability. The CV% for CD4 count was 3.5%, 8.4% and 20.1 whilst bias was 7.0, 13.5 and 8.2 for NRL, Lancet and GSH respectively. CD4% had even stronger CV% for all three laboratories. Inter-laboratory bias for Lancet/NRL was -31.5; -64.3 for Lancet/GSH and -38.2 for NRL/GSH at 12hours for CD4 count with only Lancet/GSH having a clinically interchangeable limit of agreement. At 24hours, the trends were similar, possibly confirming stability of CD4 between 12 and 24hours. An assessment of disease misclassification at HAART initiation threshold was performed. The agreement was 81.1% for Lancet/NRL, 88.7% for Lancet/GSH and 77.4% for NRL/GSH corresponding to Kappa values of 0.64, 0.77 and 0.55 respectively. McNemar test for paired proportions revealed that there were no differences between the laboratories when it came to initiating HAART. Conclusions: whilst intra-laboratory variability is minimal, there is some significant inter-laboratory variation of CD4 count and CD4% at the laboratories used in Swaziland. Swaziland should ensure standard SOPs, on -going training and continuous quality improvements for all national laboratories and ensure standards are on par with international recommendations. The national HIV guidelines should possibly enforce two different CD4 counts in decision making to reduce systematic errors. Meanwhile, clinicians should continue to use their clinical judgment in cases of suspicious CD4 count results. / Dissertation (MSc)--University of Pretoria, 2011. / School of Health Systems and Public Health (SHSPH) / Unrestricted
2

Comparison Of Compressive Strength Test Procedures For Blended Cements

Ulker, Elcin 01 September 2010 (has links) (PDF)
The aim of this thesis is to twofold, in order to demonstrate the variabilities that can be faced within the compressive strength of blended cements, one blended cement namely CEM IV / B (P-V) 32.5N is selected and the 28-day compressive strength is obtained by 16 different laboratories following TS EN 196-1 standard. Later, to show the variabilities that could be faced by different standards, three different cement types were selected and their compressive strengths are determined following two procedures first with TS EN 196-1, later with similar procedure described in ASTM. The strength of cement is determined by TS EN 196-1 in Turkey that is the same for all types of cements. However, American cement producers use different standards for testing the strength of Portland cement and blended cements. The main difference is the amount of water utilized in producing the cement mortar. It was observed that for Portland and Portland composite cements / there is not any significant difference in between the compressive strength results of cement mortars prepared by both methods. However, for pozzolanic cements, there is much deviance in the compressive strength results of cement mortars prepared by TS EN 196-1.
3

Development of a multi-collector inductively coupled plasma massspectrometry method for measurement of stable sulphur isotope ratios in aerosol sulphate

Rodiouchkina, Katerina January 2018 (has links)
Sulphur stable isotope ratios are useful tracers in geological and environmental studies. They can for example be used to trace the origin of atmospheric sulphate aerosols, because anthropogenic sulphate and natural sulphate have distinguishable δ34S-values (δ34S value of approximately +0 to +8 ‰ for anthropogenic and approximately +12 to +19 ‰ for natural). This is useful for climate modelling research, due to the net cooling effect of aerosol sulphate. In the present study a Nu Plasma II (Nu Instruments) multi collector inductively coupled plasma mass spectrometer (MC-ICP-MS) method for measuring stable sulphur isotope ratios in low sulphur content samples, such as sulphate aerosols, was developed. The method was then applied to a sulphate aerosol sample collected in the Maldives.Most of the measurements were performed at high resolution, due to the interferences on 33S. Heated spray chamber coupled to a desolvating membrane, Aridus II (Cetac), increased the sensitivity and reduced interferences notably compared to wet plasma mode. Aridus II gave more stable measurements than DSN-100 (Nu Instruments). Determinations of δ34S for IAEA S1, S3, and S4 were accurate and the determined δ34S-value of the CIT #39 seawater standard (21.05 ± 0.36 ‰, 2SD, n=42) was comparable with published data. In general, Si internal standardization correction increased precision ~2.5 times compared to non-corrected values. The δ34S-value for the sulphate aerosol sample was determined to 3.82 ±0.41 ‰ (2SD, n=40). Repeatability of ~62 nmol introduced sulphur (2 μg/mL) was generally 0.15 ‰ (2SD, n=5) for the SW and 0.19 ‰ (2SD, n=5) for the sulphate aerosol sample. Comparable results for the SW (20.61±0.09 ‰, 2SD, n=4) and sulphate aersosol sample (3.77 ± 0.08 ‰, 2SD, n=8) were obtained with the method applied to Neptune Plus (Thermo Fischer Scientific) MC-ICP-MS in a different laboratory. The determined aerosol sulphate δ34S-value indicated that the sampled sulphate aerosol originated from anthropogenic sources.

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