No description available.

No description available.

The planktonic foraminifera are a highly abundant and diverse group of marine pelagic protists that are ubiquitously distributed throughout the worlds’ oceans. These unicellular eukaryotes are encased in a calcareous (CaCO3) shell or ‘test’, the morphology of which is used to identify individual ‘morphospecies’. The foraminifera have an exceptional fossil record, spanning over 180 million years, and as microfossils provide a highly successful paleoproxy for dating sedimentary rocks and archiving past climate. Molecular studies, using the small subunit (SSU) ribosomal (r) RNA gene are used here to investigate the biogeographical distributions and phylogenetic relationships of the planktonic foraminifera. Biogeographical surveys of two markedly different areas of the global ocean, the tropical Arabian Sea, and the transitional/sub-polar North Atlantic Ocean, revealed significant genotypic variation within the planktonic foraminifera, with some genetic types being sequenced here for the first time. The foraminiferal genotypes displayed non-random geographical distributions, suggestive of distinct ecologies, giving insight into the possible mechanisms of diversification in these marine organisms. The ecological segregation of genetically divergent but morphologically cryptic genetic types could, however, have serious repercussions on their use as paleoproxies of past climate change. Phylogenetic analyses of the foraminifera based firstly on a partial ~1,000 bp terminal 3´ fragment of the SSU rRNA gene, and secondly on the ~3,000 bp almost complete gene supported the hypothesis of the polyphyletic origins of the planktonic foraminifera, which appear to be derived from up to 5 separate benthic ancestral lineages. The almost complete gene is sequenced here in the planktonic taxa for the first time, though amplification was problematic. In a first step to addressing a pressing need for new genetic markers to support data gained from the SSU rRNA gene, a culture system was established for the benthic foraminifera, in order to provide a reliable source of DNA for EST library construction or full genome sequencing. Finally, to overcome difficulties associated with the PCR amplification of the foraminifera, a new lysis buffer and DNA extraction procedure was developed. A highly successful buffer was created, allowing high quality DNA to be extracted from foraminiferal specimens, whilst leaving the delicate calcitic shell intact for morphological reference.

Arid environments are resource-limited, with scarcity of water the key limiting factor for plants and their associated fauna. Consequentially bottom-up forces often control food webs, influencing the whole system through high levels of competition. The Sinai Baton Blue butterfly, Pseudophilotes sinaicus, is Critically Endangered, with a tiny endemic distribution in the St Katherine Protectorate, South Sinai, an arid environment. Its range is restricted to that of its sole host plant, the near-endemic endangered Sinai Thyme, Thymus decussatus, leaving the butterfly in a highly fragmented distribution. This study looks into the spatio-temporal variations in quality and abundance of the host plant and its implications for the Sinai Baton Blue. Over the past decade the butterfly has exhibited severe population cycles, with the causes still unclear; it could be due to the fluctuating resource levels with large temporal variation in the quality of thyme and density of inflorescences. The number of flowers significantly influences the larval distribution, indicating that resources play a key role in offspring survivorship. Population viability analysis has also highlighted the importance of management techniques aimed at increasing the butterfly's survivorship. The butterfly population sizes are positively correlated with the total resource area and the number of host plants but not the distance between habitat patches. Population viability analysis also suggests that habitat area is more influential than connectivity in this system driving current dynamics. Overall this project has highlighted the urgent need for conservation focused on improving plant quality within patches in order to save this fragile species.

The Giant African Snail, Achatina (Lissachatina) fulica Bowdich, is a tropical crop pest species with a widespread distribution across East Africa, the Indian subcontinent, Southeast Asia, the Pacific and the Caribbean. It is also a known intermediate host of the rat lungworm, Angiostrongylus cantonensis, which can infect humans and cause eosinophilic meningitis. The phylogenetic position of A. fulica within the Achatinoidea and the Achatinidae was investigated using segments of the nuclear ribosomal (r) RNA cluster, actin and histone 3 genes and the mitochondrial CO1 and 16S rRNA genes. Results from molecular data support the monophyly of the Achatinidae based on the taxa surveyed as well as the morphological distinction of the Eastern Achatina (Lissachatina) from the Western and Central Achatina (Achatina); Lissachatina should therefore be elevated to genus status. The results also show non-monophyly of the Coeliaxidae, Ferussaciidae and Subulinidae; the taxonomy of these families must therefore be reassessed. The extent of genetic diversity in global A. fulica populations was also determined using an SSCP molecular marker developed from the 16S rRNA gene. Results reveal only one haplotype (C) emerged from East Africa and spread globally. The rat lungworm (Angiostrongylus cantonensis) has a parallel distribution with A. fulica, and the possible role of the snail in the spread of the parasite is investigated using a molecular marker derived from the small subunit (SSU) rRNA gene. A survey of the parasite within the route of dispersal of A. fulica detected A. cantonensis only in the Philippines and the French Polynesian territory of Tahiti, the latter of which being the first reported case of A. cantonensis infection for Achatina fulica in that territory. Due to the limited sampling of the snail and the patchy distribution of the parasite, there are insufficient data at this time to assess the role of Achatina fulica in the spread of Angiostrongylus cantonensis.

Neighbouring plants in the locale of an individual plant may help or hinder it in the task of defence against herbivores, depending on their levels of defence (chemical or physical), and their interactions with potential herbivores. Such ‘neighborhood effects’ are part of the complex network of species interactions that structure ecological communities. This thesis sets out to test whether there are neighbourhood effects on insect herbivory among the plants of the Al Jabal Al Akhdar region of Libya. Having chosen to concentrate on the two main species of three study sites, Juniperus phoenicea and Pistacia atlantica, nine plots were mapped in detail and the insect herbivores sampled from focal plants, and then from all plants. Leaves were sampled for chemical analysis of their phenol (tannin) content. The set of insect herbivores collected from plants in the plots were identified to species using the expertise of the staff of the Natural History Museum in London. Some insects recorded are new to Libya, and there are several species not previously recorded as feeding on either of the two plant species studied. Tannin levels were much higher in Pistacia than in Juniperus, and there were effects of elevation as well: plants from middle elevation plots had the highest levels, while those from the lowest elevation at the coast had the lowest levels of tannins. There were clear effects of neighbouring plants on the insects of individual plants, in both Pistacia and Juniperus. These were much more complex effects in Pistacia, but both sets of predictors of insect herbivore density or species richness contained clear signs of neighbourhood effects, where the existence of close neighbours reduced the herbivore load on individual plants. There were no signs of any protective neighbourhood effects of tannin levels. Thus in the plant communities of Al Jabal Al Akhdar, associational avoidance appears to be the major mechanism of neighbourhood effects, rather than associational resistance.

No description available.

Neocalanus plumchrus is the most common copepod in the Northeast Pacific, and as such plays in important role in the ecosystems of that area. The bulk of N. plumchrus’ annual life cycle is spent in a dormant overwintering state, and little is known of its ecology, behaviour, or physiology during that period. The goal of this thesis is to describe the physiological changes that occur during the overwintering period, and explain how they interact with the physical environment to produce observed life history patterns. Lipid stores in N. plumchrus were primarily wax esters, and were in highest abundance in overwintering stage 5 copepodids. Consumption of wax ester stores began approximately two months prior to moulting in situ. Rates of lipid use in the in situ population and a number of laboratory incubations ranged from 0.3 - 1% d⁻¹ , with 22 - 60% of total wax ester reserves used prior to moulting, presumably to fuel gonadogenesis. Concurrent measurements of protein content and glutamate dehydrogenase activity (an enzyme involved in protein catabolism) did not show any significant protein use during overwintering. Incubation experiments suggest that N. plumchrus has some concept of the time of year (i.e. an endogenous clock), but the use of external cues cannot be ruled out. It is often assumed that the abundant lipids found in calanoid copepods play some role in buoyancy regulation. However, lipids are generally more compressible, and more thermally expansive than seawater, which means that neutral buoyancy will be inherently unstable. A simple model of mass density shows that (i) individuals will only be able to stay at depth if they are able to diagnose where they are neutrally buoyant, and (ii) the buoyancy properties of an individual are extremely sensitive to its chemical composition. In the Strait of Georgia, depth-specific measurements of abundance showed a shift towards deeper depth distributions over the course of the overwintering period. Model results suggest that lipid use could be responsible for those changes, though deep water renewal events that occur regularly in the Strait of Georgia in winter may also have been partially responsible. / Graduate

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No description available.