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<p>Recently, the focus of metal toxicity has shifted from concentrations within the aqueous environment to bioaccumulation within the organism. In this regard, the Biotic Ligand Model (BLM) relates the binding of metal at specific toxic sites (“ligands”) to eventual toxicity, whereas the Tissue Residue Approach (TRA) relates metal burdens at whole body, tissue, or subcellular levels to eventual toxicity. However, much less is currently known regarding Ni in comparison to other metals. This thesis addresses this knowledge gap by evaluating the use of Ni bioaccumulation as a predictor of toxicity in a number of fish and invertebrate species; bioaccumulation endpoints examined included Michaelis-Menten uptake parameters (K_d, B_max), a BLM parameter (log K_NIBL values) and critical body residues (CBR50 values)</p> <p>More sensitive species exhibited higher binding affinities and lower binding capacities for Ni. In invertebrates, a strong overall correlation was observed between log K_NiBL values for whole organism binding and acute toxicity to the extent that measurement of toxicity was an acceptable alternative to measurement of binding affinity, and vice versa. However, in two teleosts, the same comparison showed that a Ni BLM built on bioaccumulation would be more protective than one built on toxicity. The results further validated a central concept of the BLM - that short term metal bioaccumulation is predictive of longer term toxicity. Acute (96-h) Ni bioaccumulation predicted chronic (15 or 30-day) mortality in both salt and fresh water. In the latter, acute (96-h) sub-cellular bioaccumulations of Ni in either biologically active (BAM) or biologically inactive metal pools (BIM) of one fish species (round goby) were also predictive of 30-d mortality. However, goby were more sensitive to Ni bioaccumulation in the BAM versus BIM fraction.</p> <p>This thesis advances the use of bioaccumulation as a predictor of Ni toxicity and may have implications for metal toxicity frameworks such as the BLM and TRA.</p> / Doctor of Philosophy (PhD)

Water hyacinth (Eichhornia crassipes) is a floating non-native plant that has been reoccurring in Lake Chapala, Jalisco, Mexico for more than 100 years. In this research, I explore the effects of water hyacinth on freshwater ecosystems worldwide and specifically on Lake Chapala. In chapter 1, I reviewed studies conducted on water hyacinth worldwide and found that the effects of water hyacinth on water quality are similar but the magnitude of effects is dependent on the percent cover and potentially the spatial configuration of water hyacinth mats. Water hyacinth's effect on aquatic invertebrates, fish, and waterbirds is less predictable and dependent on conditions prior to invasion. In chapter 2, I tested for relationships between percent water hyacinth cover and waterbird abundance, species diversity, community composition, and habitat use. In general, I found a weak positive relationship or no relationship between these variables. In Chapter 3, I monitored habitat use by American Coots (Fulica americana) in a variety of habitats around Lake Chapala. I found that the time spent in water hyacinth positively corresponded to the percent water hyacinth cover and that the time foraging in water hyacinth was positively related to the time spent in water hyacinth. In Chapter 4, I compared invertebrate assemblages in open water to those within and at the edge of water hyacinth mats, emergent vegetation, and submerged trees. I also examined invertebrate assemblages within the roots of water hyacinth plants and compared assemblages between patch and shoreline water hyacinth plants. I found that density and taxonomic richness of water column invertebrates were generally higher in association with water hyacinth, but that mean percent cover of water hyacinth affected the magnitude of differences among habitats and vegetation types. I did not find significant differences in root invertebrate density and taxonomic richness between patch and shoreline water hyacinth plants. In chapter 5, I discuss how water hyacinth affected dissolved oxygen and water transparency on a small, localized scale, but was not the driving factor for seasonal differences. The overall results suggest that water hyacinth had a minimal ecological effect on Lake Chapala during this study. / Ph. D.

Mercury poses significant challenges to human health and fisheries management. Historical industrial practices in Waynesboro, Virginia left portions of the Shenandoah River basin contaminated with mercury and stringent health advisories for fish consumption. I investigated processes affecting the bioaccumulation of mercury in Catostomus commersoni, Ictalurus punctatus, Lepomis auritus, and Micropterus dolomieu by studying food habits, total mercury and methylmercury in common prey items, and bioaccumulation dynamics of methylmercury in the mercury contaminated South River and South Fork of the Shenandoah River and uncontaminated North River. Additionally, I evaluated sexual and seasonal variations of total mercury in M. dolomieu in the South Fork of the Shenandoah River. Algae, aquatic insects, crayfish, detritus, and fish accounted for 75-97% of the diet. Total mercury in aquatic invertebrates and forage fish in contaminated rivers ranged from 66.7-398.3 and 198.0-594.9 ng/g wet weight, while total mercury in aquatic invertebrates and forage fish in the reference river were 4.4 and 29.3 ng/g. Model simulations indicated that dietary pathways accounted for 87% of methylmercury uptake by fish in contaminated rivers, but only 57% in the reference river. Total mercury in M. dolomieu was 19-20% higher in females than males and 14-21% higher during spring than summer and fall. Results of this study indicate that bioenergetics-based bioaccumulation models are valuable tools for evaluating field data, identifying processes critical to contaminant accumulation, and comparing outcomes of alternative management options associated with pollution control, ecosystem management, and/or restoration activities for management guidance prior to costly expenditures. / Master of Science

An increase in public environmental awareness and a decrease in resources within government regulatory agencies have led to a larger interest in volunteer biomonitoring programs. Government agencies are currently using volunteer data for official purposes with increasing frequency, but questions have been raised about the validity of the data collected by volunteers who have only limited training and experience. Therefore, we conducted a detailed study to assess, modify, and validate the Virginia Save-Our-Streams (SOS) program, which is a volunteer organization collecting macroinvertebrate data. Sites were sampled using professional methods concurrently with volunteers who utilized the SOS protocol. The volunteer samples were retained for further laboratory analysis. In addition, numerous sites previously sampled by volunteers were re-sampled using professional methods. The data were statistically analyzed to determine if the results of volunteers and professional aquatic biologists were correlated and if they arrived at the same conclusions about ecological condition. It was determined that the Virginia SOS method, and probably other similar volunteer methods, consistently overrate ecological condition. This means that streams impaired by pollution could go unreported, if they are monitored exclusively by volunteers. The cause of this overestimation was determined to be the overly simplistic SOS metric, which is based solely on the presence or absence of taxa. The SOS protocol for data analysis was made more quantitative by developing a multimetric index that is appropriate for use by volunteers. The SOS sampling protocol was modified slightly to obtain actual counts of the different kinds of macroinvertebrates, which allowed for calculation of metrics. Sorting effort and taxonomic level of identification were not changed so that currently participating volunteers would not be excluded because of the need for expensive equipment or advanced technical training. The modified SOS protocol was evaluated by a different set of concurrent samples taken by volunteers and professionals, but using the same statistical techniques. The modified SOS protocol proved to be feasible for volunteers. The new SOS multimetric index correlated well with a professional multimetric index. The conclusions about ecological condition derived from the volunteer multimetric index agreed very closely with those made by professional aquatic biologists. This study demonstrated that volunteer biomonitoring programs can provide reliable data, but every volunteer program needs to be thoroughly validated by statistical comparisons to the professional methods being used in that area. / Master of Science

Non-native species richness has been observed to peak at mid-temperate latitudes, shaping a pattern of richness and abundance that is distinct from native species patterns that peak in the tropics. Stronger species interactions, and therefore biotic resistance, may lower invasion success in the tropics and help explain the discrepancy between native and non-native richness and abundance. To test the hypothesis that strong predation and competition in the tropics could limit invasion success, we conducted a distributed experiment on sessile marine invertebrate communities in four regions spanning 47-degrees latitude of the eastern Pacific Ocean. We manipulated predator access and resource availability at 12 sites and sampled experimental communities in early and late stages of assembly. Overall, our results suggest that biogeographic location, assembly timescale, and predation interactively shape invasion success across latitude. Strong predation reduced richness of non-native species in the tropics at both assembly timescales but increased non-native species richness in the subtropics during early assembly. Predation also increased non-native abundance in the tropics by late-stage assembly and shaped non-native composition at both assembly stages. Effects of predation at higher latitudes were weak or undetectable, and increasing resource availability never had a positive impact on non-native richness or abundance at any latitude. Further, non-native richness was greater at early relative to late assembly stages at mid to low latitudes and was consistently low in our high latitude region at both timescales. In a complementary experiment, short-term predator exposure reduced non-native abundance in Panama, further confirming the influence of predation in this tropical region. Our results highlight important biogeographic differences in invasion dynamics and disentangle local mechanisms that can shape regional patterns. / Biology

This thesis provides a contribution to the knowledge on the effects of deglaciation on alpine stream ecosystems, taking into account also the hydroecological influence of thawing permafrost and paraglacial features. With a focus on the European Alps, a review is provided on the climate changes and shifts in the cryosphere (snow, glaciers, permafrost), the related changes in hydrology, geomorphic processes and the physical and chemical habitat of alpine river networks, and the consequent shifts in stream communities and food webs. A conceptual model is provided to summarize the complex interactions and the cascading effects triggered by deglaciation on hydrology, habitat and biota of alpine streams, that can be useful for educational purposes and to help the scientific community to contextualize these issues to other alpine areas. Deglaciation induces homogenisation of river networks, loss of biodiversity, and shifts in primary and secondary production, functional diversity and food webs. The scarce published studies on streams influenced by permafrost provide hints on the role of thawing rock glaciers (i.e. evident form of mountain permafrost) in shaping the ecology of freshwaters, and reveal important research gaps. To increase the knowledge on this topic, different alpine streams fed by waters of different origin were selected in two subcatchments (Zay, Solda) of a deglaciating area of the Central Italian Alps (Solda Valley), and their habitat conditions and benthic invertebrate communities were investigated over a two-year period. Rock glacier-fed streams could be distinguished from those fed by glaciers, groundwater and those of mixed origin because of their constantly clear and very cold waters, stable channels, and high concentrations of ions and trace elements that increased as summer progressed. Furthermore, the Zay rock glacier strongly influenced the glacier-fed stream through an intense export of solutes, which become progressively more relevant towards the end of summer. This influence was also due to the contribution of a proglacial lake and a moraine body, that both strongly decreased the glacial influence along the glacier-fed stream before its confluence with the rock glacier outflow. The wide range of habitat conditions revealed to strongly influence the benthic invertebrate communities in the study area. Channels with groundwater (krenal) and mixed (glacio-rhithral) exhibited a higher taxa richness and diversity. Peaks of abundance and biomass in the catchment were recorded just downstream the talus body, in the upper glacio-rhithral channel. Chironomidae from the cold-adapted genus Diamesa were dominant in the proglacial sections (upper kryal) of the glacier-fed streams. The proglacial lake, the moraine body and the rock glacial tributary at Zay contributed to the amelioration of the environmental features of the glacier-fed stream (lower kryal), boosting high invertebrate biomass and abundance and causing shifts in the community composition (e.g. increased Orthocladiinae and other Diamesinae chironomids, abundant Trichoptera). The two rock glacial communities differed considerably between each other. In fact, the community of the Zay rock glacial stream was partially influenced by the seepage of glacier waters, and resembled those of the surrounding lower kryal. On the contrary, the Solda rock glacial stream, detached from any glacier influence, hosted a rich and diverse community which resembled those of glacio-rhithral and krenal, even though with a higher abundance of Diamesa. Overall, the results of this thesis showed that in the advanced phases of glacier retreat, paraglacial landforms and permafrost can increasingly contribute to the riverscape diversity and shape the ecology of river networks. Because of their unique environmental settings, rock glacial streams should be considered a distinct alpine stream habitat, acting in deglaciating catchments as stepping stones that enhance the upstream colonisation of non-glacial communities following glacier retreat. At the same time, they might represent cold refugia for cold-stenothermal and/or typically glacial taxa when glaciers will be disappeared, because of the slower thawing rate of rock glacier ice. In this context, the presence of Diamesa kryal specialist species in rock glacial streams deserves further investigation, in order to understand the potential conservation value that these habitats may have in buffering the β-diversity reduction which is predicted in alpine areas as a consequence of glacier loss.