"Dipeptidyl peptidase-IV Activity and/or Structure Homologues"(DASH) represent a newly defined group of multifunctional molecules, typically bearing dipeptidyl peptidase-IV- like hydrolytic activity. Dipeptidyl peptidase-IV (DPP-IV) cleaves out X-Pro dipeptides from the N-terminus of peptides. Other molecules carrying similar enzyme activity, such as Fibroblast activation protein (FAP), DPP-II, DPP8 and DPP9 or even DPP-IV structure-like but hydrolytically inactive molecules (DPP6 and DPP10) also belong to this group. Recent knowledge suggest a substantial role of DASH in cancer pathogenesis. The aim of this study is a preparation of a biological model and its use for understanding the mechanisms of interaction(s) between transformed glial cells and stroma in the processes of origin and development of tumors derived from neuroectoderm. Stable transfected human glioblastoma cell lines with inducible gene expression of DPP-IV, Fibroblast activation protein and their enzymatically inactive mutated forms, were prepared within the project. Prepared cell lines are used as a tool for studying not only the "autocrine" importance of DPP-IV and FAP for the expressing cells in in-vitro, but also for their potential "paracrine" effect(s) within the tumor microenvironment after homotopic implantation into the...

A finalidade deste estudo foi verificar a resistência ao deslocamento de restaurações de Classe IV, ressaltando a sua capacidade de retenção com e sem a associação de pino(s) dentinários, bem como a comparação entre dentes humanos e bovinos, visando este último como possível substrato a dentes humanos em testes laboratoriais. Foram realizadas 60 cavidades de Classe IV com 6mm no sentido incisocervical e 3mm no sentido mesiodistal, tanto em dentes humanos como bovinos, com um bisel de 1mm de extensão, as quais foram divididas nos seguintes grupos: · G1- restaurações sem pino, sem condicionamento + adesivo (controle); · G2- restaurações com condicionamento ácido + adesivo; · G3- restaurações com um pino, sem condicionamento ácido + adesivo; · G4- restaurações com dois pinos, sem condicionamento ácido + adesivo; · G5- restaurações com um pino, com condicionamento ácido + adesivo; · G6- restaurações com dois pinos, com condicionamento ácido + adesivo. A aplicação do sistema adesivo (Single Bond) e a inserção da resina composta (Z 100) deu-se de acordo com as instruções do fabricante. Após a realização das restaurações, os corpos-de-prova foram armazenados por um período de 72 horas a 37+1oC antes de serem levados à máquina de ensaios universal para serem submetidos à carga de compressão. A análise estatística aplicada aos resultados obtidos segundo as condições experimentais em que foi realizado este trabalho permitiu as seguintes conclusões: - a presença de um ou dois pinos rosqueados em dentina apresentou tendência em aumentar a resistência ao deslocamento das restaurações de Classe IV quando associadas ao sistema adesivo; - a utilização de um pino dentinário proporcionou aumento na retenção das restaurações de Classe IV, todavia, não suficiente para suplantar estatisticamente as restaurações adesivas sem essa variável; - a utilização de dois pinos dentinários proporcionou aumento estatisticamente significante na resistência ao deslocamento das restaurações,em relação às realizadas apenas com o sistema adesivo; - não existiu diferença na resistência ao deslocamento das restaurações de Classe IV realizadas em dentes bovinos e em dentes humanos. / The goal of this study was to verify the resistance to displacement of Class IV restorations, emphasizing their retention capacity with and without the association of retentive pins (Pinlock), as well as the comparison between human and bovine teeth, aiming at the latter as a possible substrate to human teeth in laboratory tests. Sixty Class IV cavities with 6 mm in the incisal-cervical plane and 3 mm in the mesio-distal plane and with bevel 1mm were performed, both on human and bovine teeth, which were divided into the following groups: · G1 - restorations without acid conditioning and pin + adhesive (control); · G2 - restorations with acid conditioning + adhesive; · G3 - restorations without acid conditioning + one pin + adhesive; · G4 - restorations without acid conditioning + two pins + adhesive; · G5 - restorations with one pin + acid conditioning + adhesive +; · G6 - restorations with two pins + acid conditioning + adhesive. The application of the adhesive system (Single Bond) and the insertion of composite resin (Z100) were according to the manufacturer's instructions. Following restoration procedures, the specimens were stored for a period of 72 hours at 37+ oC prior to being taken to the Universal testing machine, so as to be submitted to compression load. The statistical analysis applied to the results obtained, according to the experimental conditions in which this study was performed, allowed the following conclusions:- the presence of one or two dentin-threaded pins showed a trend in increasing the displacement resistance of Class IV restorations when associated with the adhesive system; - the utilization of one dentinal pin afforded an increase in the retention of Class IV restorations, nevertheless, not sufficient to supersede the adhesive restorations statistically without this variable; - the utilization of two dentinal pins afforded a statistically significant increase in the displacement resistance of restorations, in relation to those performed only with the adhesive system; - there was no difference as to displacement resistance of Class IV restorations performed on both bovine and human teeth.

Orientador: Andre Leonardo Chevitarese / Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Filosofia e Ciencias Humanas / Made available in DSpace on 2018-08-04T03:40:13Z (GMT). No. of bitstreams: 1 Batagello_Rodrigo_M.pdf: 345815 bytes, checksum: 9a45276a525f15e1330c0c84e8f65b40 (MD5) Previous issue date: 2005 / Resumo: Nas últimas décadas os estudos sobre as obras de Platão têm mudado e muitos deles apontam que é necessário rever algumas das perspectivas que foram adotadas para compreender os Diálogos deste filósofo. Estas abordagens criticam o lugar comum que defende que toda a obra platônica estaria totalmente comprometidas com as idéias metafísicas de Platão e não teriam ou teriam apenas uma negligenciável dimensão histórica. Debatendo e assumindo algumas dessas abordagens, o principal objetivo deste trabalho é investigar as possíveis relações entre a famosa obra de Platão, a República, e os problemas da organização militar ateniense, que tiveram lugar depois da Guerra do Peloponeso. Neste sentido, esta pesquisa pretende compreender como a República relaciona-se com o problema da educação militar e com as mudanças na concepção de cidadão-soldado que tiveram grande importância para as cidades gregas no século IV / Abstract: In the last decades studies about Plato¿s opera have been changing and many of them show that it is necessary to review some points to understand the philosopher Dialogues. The current approaches criticize the common place that defends that all platonic opera were committed with Plato¿s metaphysical ideas and could not have only a minor historical dimension. Discussing and assuming some of these approaches, the main objective of this study is to investigate possible relation with the famous Plato¿s opera, Politeia, and the Athenian military organization that appeared after Peloponnesian War. So, this research intends to understand the relationship between the Politeia with the military educational problems and changes in the citizen-soldier conception that were very important for the greek cities in the IV century / Mestrado / Historia Cultural / Mestre em História

in English - Diploma thesis - Filip Košťál The topic of this thesis is "Legal Regulation of Banking Activities". Firstly, the thesis describes reasons and theoretical basis for regulation of banks. Next, the thesis focuses on three levels of regulation of banking, i.e., regulation within the Basel Committee on Banking Supervision, which prepares recommendations, which, however, are mostly followed by regulatory activities at the European Union level. Last but not least, the thesis also focuses on Czech legislation, which is, together with directly applicable regulations of the European Union binding for banks carrying out activities in the Czech Republic. The main purpose of this thesis is to provide the reader with a consolidated insight into all three levels of banking regulation and point out potentially problematic points arising out of it. The thesis also illustrates some of the aspects of banking regulation on specific examples from the Czech banking sector. The abovementioned regulatory measures deal mostly with prudential rules for banks. The thesis, however, focuses also on some other selected areas of banking regulation, namely banking secrecy and its release with regard to credit registers, obligations of banks arising out of the act on selected measures against legitimisation of...

Abstract Tumor invasion and metastasis are the major causes of treatment failure or death for carcinoma patients. Matrix metalloproteinases (MMPs) are zinc dependent endopeptidases implicated in tumor invasion and metastasis. The expression of MMP-2 has been previously linked to invasiveness of carcinoma cells. The MMP-2 immunoreactive protein was studied here in squamous cell carcinoma of the utrine cervix and in adenocarcinoma of the breast by using a specific monoclonal antibody in immunohistochemical stainings. Immunoreactive protein of latent MMP-2 was found to be an early event in neoplastic transformation of the cervix in 60 patients. All cases of early stage cervical carcinoma expressed the latent MMP-2 protein, suggesting that MMP-2 could be a prerequisite for invasive behavior. In early stage cervical carcinoma the high score of MMP-2 expression seemed to be associated with poor histological differentiation and lymph node metastases. The intensitivity (score) of the immunoreaction was not, however, associated with clinical behavior of this disease. New predictive markers would be useful in selecting breast carcinoma patients to different modalities of adjuvant therapy. The MMP-2 protein has been found in breast carcinoma tumor cells in immunohistochemical analyses. MMP-2 has been found to be expressed in breast carcinoma in some preliminary studies, but there are no reports so far that would show a correlation of MMP-2 to survival in breast carcinoma. In the current study comprising 373 patients the expression of MMP-2 protein was found immunohistochemically in primary breast carcinomas. It is shown here for the first time that immunoreactive protein of MMP-2 in primary breast carcinoma is associated with a shortened relapse-free survival (RFS) or relative overall survival (OS). MMP-2 correlated to the risk of failure during the anti-estrogen adjuvant therapy in postmenopausal breast carcinoma patients with axillary lymph node metastasis without a high tumor burden. It was also found here that premenopausal patients with a node positive breast carcinoma showing MMP-2 positivity relapsed early after the primary operation. Young patients (< 40 years) with MMP-2 positive tumors had a poor outcome when compared to other node-positive premenopausal breast carcinoma patients. A patient group with a high risk for an early relapse was identified from node-positive, premenopausal breast carcinoma patients. In conclusion, the present data show for the first time MMP-2 immunoreactive protein to be a prognostic factor in breast carcinoma, indicating further studies to explore the value of this enzyme in clinical decision making.

Class of 2014 Abstract / Specific Aims: To determine the cost of treatment of oral and intravenous n-acetylcysteine (IV NAC) in acute acetaminophen (APAP) toxicity using the length of treatment and length of hospital stay. Methods: A retrospective chart review of Arizona Poison and Drug Information Center electronic records from 2009-2012 and January-June 2013 were evaluated. The following information was collected: age, sex, use oral or intravenous NAC, length of treatment, length of hospital stay (intensive care unit (ICU) and non-ICU) and use of antiemetic. The mean length of stay (MLOS) was calculated for each group as well as the cost of IV and oral NAC. These means were then compared using t-test for independent groups to test for significance. The average total cost of IV and oral NAC treatment was calculated by using monetary values from primary literature. A sensitivity analysis was performed to test the possible effects of an increase or decrease of the final costs by 5 to 10%. Main Results: Patients (≥18 yrs) being treated with IV or oral NAC for acute APAP toxicity (≤8 hours prior to ingestion) were included in this study. A total of 47 patients met the inclusion criteria. Length of hospital stay was shorter in patients receiving IV NAC (42.5% 24-24hr; 37.5% 48-72hr) compared to patients receiving oral NAC (28.6% 48-72hr, 71.4% >72hrs; p<0.001). Total cost of ICU/non-ICU stay in patients receiving IV NAC ($8,720/$3010) was less than patients receiving oral NAC ($12,321/$4703); however, cost of IV NAC-extended (37hrs) in ICU/non-ICU ($13,153/$5535) was greater than oral NAC. The sensitivity analysis performed demonstrated that an increase or a decrease by 5 to 10% in change of cost does not affect our final conclusion. Conclusion: The cost of treatment of IV NAC is lower due to shorter LOS of patients treated with IV NAC (p<0.001). However, when an extended course of treatment is medically necessary for patients on IV NAC then the cost of treatment with IV NAC exceeds the cost of treatment with oral NAC.

Class of 2016 Abstract / Objectives: Pain management in the pediatric population is crucial when providing emergency medical care, as inadequate pain control is a significant cause of morbidity and mortality. The use of adjunctive therapy can potentially decrease opioid requirements, thereby reducing potential opioid related adverse effects. The purpose of this study was to evaluate the use of adjunctive therapy and impact on morphine dose requirements for pediatric pain management in the emergency department (ED). Methods: This study was an IRB approved retrospective review of pediatric patients ages 1 to 18 years, who received intravenous (IV) morphine therapy in the ED. Patients were excluded based on opioid-tolerance (using opioids prior to ED visit), diagnosis of sickle cell disease, and oncologic disorders. Data collection included baseline demographics, medical diagnoses and comorbidities, morphine total dose by weight, type, dose by weight and frequency of adjunctive analgesia agents, and pain scores. Results: The use of adjunctive analgesia in addition to morphine did not reduce the total morphine doses given, repeat morphine dose requirements, admission rates, or length of stay but did increase the time to a repeat dose of morphine. In those patients who received adjunctive analgesia before morphine, we saw a statistically significant decrease in the total amount of morphine received, total morphine doses given, repeat morphine dose requirements, and admission rates. Conclusions: In pediatric patients who require pain management in the ED, adjunctive analgesia should be given before morphine to reduce the amount of morphine required.

Este trabajo intenta ser un breve estudio gramatical de la lengua castellana como se usa en la Crónica de don Fernando IV. El estudio se basa en la edición de la Biblioteca de Autores Españoles de las Crónicas de los reyes de Castilla. Los ejemplos vienen de los veinte capítulos de la Crónica de don Fernando IV., pp. 93-102. El original de esta crónica fue escrito de 1340 a 1352. Hay muchos códices de ella, y la impresión de Sebastián Martínez en el año 1554 fue sacada de alguno de éstos. El editor de la presente edición apunta que la de 1554 tenía muchas variantes, faltas y alteraciones, y por eso él ha deducido su texto del códice de la Biblioteca Naclonal, comparándola con el del Escorial y el del señor Duque de Osuna, y también con la impresión de Sebastián Martínez. Algunos suponen que el autor de esta crónica es Fernán Sánchez de Valladolid, o de Tovar, pero la Academia Española la atribuyó a Juan de Villaizan. El sistema de ortografía empleado por el editor es muy variable, y él lo justifica así: "No extrañen nuestros lectores la irregularidad é inconsecuencia que notarán en el sistema, ó mejor dicho, en la falta total de sistema de la orto-grafia que nemos empleado. La variedad con que escribimos nombres y verbos, unas veces respetando su forma antigua, otras modernizándolos, usando indistintamente de mayúsculas o minúsculas, y desatendiendo completamente los signos prosódicos de las voces, proviene de la misma incorrección que se ve en los origlnales. Hállanse en ellos también blancos y lagunas, que no siempre hemos podido llenar, porque en todos se advierte la misma falta. Trabajoso hubiera sido, pero posible al fin, establecer un sistema uniforme de escritura; sin embargo, habríamos privado á los textos de su carácter, que precisamente consiste en ese desórden y confusión." Esta irregularidad de parte del señor Rosell se nota también en sus fechas. Advierte al lector en la página 119, "La edición de 1554 y los códices que tenemos á la vista adelantan un año en el computo cronológico, como lo demostró el señor Benavides en la reimpresión de esta crónica, hecha por la Academia de la Historia el año 1860. Hemos hecho la corrección en los capítulos anteriores; pero desde aquí conservarémos el yerro del original. Téngase presente." Más tarde, en la página 162, lo reítera; "Respecto á la equivocacion de la cronología, ya hemos hecho la oportuna advertencia." ¿No habría sido más conveniente o seguir el original en todo o corregirlo completamente? / Arts, Faculty of / Central Eastern Northern European Studies, Department of / Graduate

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Antisocial Personality Disorder (APD) represents a controversial diagnoses which has gone through many revisions over the past 25 years and is scheduled to be revised again for the DSM IV. A comprehensive survey was composed of APD criteria from the DSM II, DSM III, DSM III-R, PCL-R, Psychopathic Personality Disorder, and Dyssocial Personality Disorder. The survey was completed by 321 forensic psychiatrists based on which criteria they believed to be the most prototypical of antisocial personality. The results identified four factors: irresponsibility, unstable self image, and unstable relationships; manipulation and lack of guilt; aggressive behavior; and nonviolent juvenile delinquency. A diagnostic set composed of the most prototypical criteria was proposed for the DSM IV diagnosis of APD.