"October 2002" Includes bibliographical references (leaves 239-277)

"October 2002" / Includes bibliographical references (leaves 239-277) / xxii, 278, [18] leaves : ill. ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Thesis (Ph.D.)--University of Adelaide, Dept. of Physiology, 2002

Copies of author's previously published articles inserted. / Bibliography: leaves 169-219. / 224 leaves : ill. ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Describes experiments to determine what factors affect the outcome of the reflex response of the jaw closing muscles to peridontal mechanoreceptive stimulus. The reflex responses of the human masseter were investigated by applying force using different stimulus profiles. It was shown that when the force profile had little or no fast component, the likelehood of eliciting an exitatory peridontal masseteric reflex increased. It is concluded that the shape of the stimulus profile, the location of the stimulating probe and the presence of preload are the main factors that determine the exitatory reflex response of the jaw closing muscles. / Thesis (Ph.D.)--University of Adelaide, Dept. of Physiology, 2000

Copies of author's previously published articles inserted. Bibliography: leaves 169-219. Describes experiments to determine what factors affect the outcome of the reflex response of the jaw closing muscles to peridontal mechanoreceptive stimulus. The reflex responses of the human masseter were investigated by applying force using different stimulus profiles. It was shown that when the force profile had little or no fast component, the likelehood of eliciting an exitatory peridontal masseteric reflex increased. It is concluded that the shape of the stimulus profile, the location of the stimulating probe and the presence of preload are the main factors that determine the exitatory reflex response of the jaw closing muscles.

Master of Science / A mechanical stimulus producing stretch in human or animal muscle may evoke a reflex response in the muscle that tends to oppose the length change. In decerebrate preparations, limb flexion generates a tonic stretch reflex that manifests as a sustained increase in resistance (Liddell and Sherrington, 1924; Widmalm, 1976b). This tonic response is not readily apparent in the conscious human subject, however, the phasic response may be observed as a burst of action potentials produced by the synchronous firing of several motor units, and as such represents the classic tendon reflex. The analogous compound action potential in the jaw musculature may be demonstrated following a sudden downward mechanical impulse to the mandible in the human or animal subject (Goodwill, 1968; Matthews, 1976) and has been termed the jaw-closing reflex or the jaw jerk reflex, the latter so named in view of its similarity to the knee-jerk and other tendon reflexes induced by sudden stretch (Goodwill, 1968; Munro and Griffin, 1971; Tardieu, Tabary and Tardieu, 1973). The use of the term “jaw jerk” to describe this reflex may be inappropriate as it has been used to describe a sudden opening movement (Riblet and Mitchell, 1971). It would appear preferable therefore to avoid the term “jaw jerk” in in favour of less ambiguous terminology such as “jaw-closing” reflex or monosynaptic myotatic reflex potential (MSP; Widlam, 1976a and b). The jaw-closing reflex is considered a fundamental phenomenon of the facial and oropharyngeal areas (Dubner, Sessle and Stoery, 1978) as it utilises afferent and efferent components involved in the generation, learning and modulation of programmed jaw movement sequences. Thus the reflex would appear to form the basis of more complex functions such as mastication and swallowing (Sessle, 1981), although the response itself probably appears only infrequently in normal function. A downwards tap delivered to the chin in a relaxed human subject causes muscle stretch and this produces an afferent projection along group Ia and group II pathways (Figure:1) which in turn exert monsynaptic and polysynaptic influences on motoneurones in the trigeminal motor nucleus. Inter-segmental and suprasegmental projections onto alpha and fusimotoneurones located in this motor nucleus (Greenwood and Sessle, 1976; Sessle, 1977a and b) modulate ongoing motoneurone excitability thus influencing the mainifestation of evoked monosynaptic reflexes.

Master of Dental Surgery / A study of the masticatory apparatus can be divided into the following sections:- Bony structure Teeth and supporting structures Temporomandibular articulation (and ligaments) Muscles of mastication (elevation and depression) Muscles of facial expression and deglutition Neurology Vascular and lymphatic supply This work is concerned with the muscles of mastication and the positions and movements of the mandible for which they are responsible. It is of course impossible to divorce this particular aspect from the others, as they are all completely interdependent, and, in particular, a study of the neurology is essential for an understanding of the function of the muscles of mastication. When all parts of the apparatus are in the correct functional relationship they operate with the greatest efficiency and the least effort. The jaws and neuromuscular system should be in correct relationship to avoid neuromuscular tension and damage to the component parts of the masticatory system.

Master of Dental Surgery / A study of the masticatory apparatus can be divided into the following sections:- Bony structure Teeth and supporting structures Temporomandibular articulation (and ligaments) Muscles of mastication (elevation and depression) Muscles of facial expression and deglutition Neurology Vascular and lymphatic supply This work is concerned with the muscles of mastication and the positions and movements of the mandible for which they are responsible. It is of course impossible to divorce this particular aspect from the others, as they are all completely interdependent, and, in particular, a study of the neurology is essential for an understanding of the function of the muscles of mastication. When all parts of the apparatus are in the correct functional relationship they operate with the greatest efficiency and the least effort. The jaws and neuromuscular system should be in correct relationship to avoid neuromuscular tension and damage to the component parts of the masticatory system.

Master of Science / A mechanical stimulus producing stretch in human or animal muscle may evoke a reflex response in the muscle that tends to oppose the length change. In decerebrate preparations, limb flexion generates a tonic stretch reflex that manifests as a sustained increase in resistance (Liddell and Sherrington, 1924; Widmalm, 1976b). This tonic response is not readily apparent in the conscious human subject, however, the phasic response may be observed as a burst of action potentials produced by the synchronous firing of several motor units, and as such represents the classic tendon reflex. The analogous compound action potential in the jaw musculature may be demonstrated following a sudden downward mechanical impulse to the mandible in the human or animal subject (Goodwill, 1968; Matthews, 1976) and has been termed the jaw-closing reflex or the jaw jerk reflex, the latter so named in view of its similarity to the knee-jerk and other tendon reflexes induced by sudden stretch (Goodwill, 1968; Munro and Griffin, 1971; Tardieu, Tabary and Tardieu, 1973). The use of the term “jaw jerk” to describe this reflex may be inappropriate as it has been used to describe a sudden opening movement (Riblet and Mitchell, 1971). It would appear preferable therefore to avoid the term “jaw jerk” in in favour of less ambiguous terminology such as “jaw-closing” reflex or monosynaptic myotatic reflex potential (MSP; Widlam, 1976a and b). The jaw-closing reflex is considered a fundamental phenomenon of the facial and oropharyngeal areas (Dubner, Sessle and Stoery, 1978) as it utilises afferent and efferent components involved in the generation, learning and modulation of programmed jaw movement sequences. Thus the reflex would appear to form the basis of more complex functions such as mastication and swallowing (Sessle, 1981), although the response itself probably appears only infrequently in normal function. A downwards tap delivered to the chin in a relaxed human subject causes muscle stretch and this produces an afferent projection along group Ia and group II pathways (Figure:1) which in turn exert monsynaptic and polysynaptic influences on motoneurones in the trigeminal motor nucleus. Inter-segmental and suprasegmental projections onto alpha and fusimotoneurones located in this motor nucleus (Greenwood and Sessle, 1976; Sessle, 1977a and b) modulate ongoing motoneurone excitability thus influencing the mainifestation of evoked monosynaptic reflexes.

The human jaw, like all other articulated body parts, exhibits small oscillatory movements during isometric holding tasks. These movements, known as physiological tremor, arise as a consequence of the interaction of various factors. One of these factors is reflex feedback from peripheral receptors. In the human jaw, receptors that innervate the periodontium are able to transduce minute changes in force. This thesis examines the contribution of these periodontal mechanoreceptors (PMRs) to the genesis of physiological tremor of the human jaw. By using frequency domain analysis of time series recorded during isometric biting tasks, the character of physiological jaw tremor can be revealed. Physiological jaw tremor was observed in force recorded from between the teeth as well as from electromyograms recorded from the principal muscles of mastication. These recordings have shown us that jaw physiological tremor consists of a frequency invariant component between 6 and 10Hz. This frequency remains unaltered under various load conditions where the mechanical resonance of the jaw would be expected to vary greatly (Chapter 2). Such findings indicate a ‘neurogenic’ origin for this tremor. A possible candidate for this neurogenic component of physiological tremor in the jaw is the reflex feedback arising from the PMRs. Using local anaesthetisation, it has been shown in this thesis, that by blocking outflow from the PMRs, the amplitude of neurogenic physiological jaw tremor can be reduced dramatically. This procedure caused a dramatic reduction in not only the mechanical recordings of tremor but also in the coupling between masseteric muscles bilaterally (Chapter 3) and between single motor units recorded from within a homonymous muscle (Chapter 4). The obvious mechanism by which periodontal mechanoreceptor anaesthetisation could reduce the amplitude of physiological tremor in the jaw would be by reducing the amplitude of the oscillatory input to the motoneurones driving the tremor. This interpretation remains controversial however as physiological tremor in the jaw can be observed at force levels above which the PMRs are supposedly saturated in their response. In light of this knowledge, the saturating characteristics of these receptors in terms of reflex output were examined. To do this, a novel stimulation paradigm was devised whereby the incisal teeth were mechanically stimulated with identical stimulus waveforms superimposed upon increasing tooth preloads. This necessitated the use of a frequency response method to quantify the reflexes. An optimal frequency for stimulation was identified and used to confirm that the hyperbolic saturating response of PMRs observed previously, translated to a similar phenomenon in masticatory reflexes (Chapter 5). These data reinforced the idea that physiological tremor in the jaw was not just a consequence of rhythmic reflex input from PMRs, as the dynamic reflex response uncoupled from the input as the receptor-mediated reflex response saturated. An alternative hypothesis was then developed that suggested the effect of PMR suppression in physiological tremor was via tonic rather than rhythmic effects on the masseteric motoneurone pool. By utilising a novel contraction strategy to manipulate the mean firing rate of the motor neuron pool at a given level of force production, data contained in Chapter 6 shows that population motor unit firing statistics influence the expression of physiological tremor, and such manipulations mimic, to an extent, the changes in firing statistics and tremor amplitude seen during anaesthetisation of the PMRs. This thesis therefore posits a mechanism whereby periodontal input influences the firing rate of motoneurones in such a way as to promote tremulous activity (Chapter 5). However, as this proposed mechanism did not explain the full extent of tremor suppression seen during PMR anaesthetisation it can therefore only be considered a contributing factor in a multifactor process. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1297555 / Thesis (Ph.D.) -- University of Adelaide, School of Molecular and Biomedical Science, 2007

The human jaw, like all other articulated body parts, exhibits small oscillatory movements during isometric holding tasks. These movements, known as physiological tremor, arise as a consequence of the interaction of various factors. One of these factors is reflex feedback from peripheral receptors. In the human jaw, receptors that innervate the periodontium are able to transduce minute changes in force. This thesis examines the contribution of these periodontal mechanoreceptors (PMRs) to the genesis of physiological tremor of the human jaw. By using frequency domain analysis of time series recorded during isometric biting tasks, the character of physiological jaw tremor can be revealed. Physiological jaw tremor was observed in force recorded from between the teeth as well as from electromyograms recorded from the principal muscles of mastication. These recordings have shown us that jaw physiological tremor consists of a frequency invariant component between 6 and 10Hz. This frequency remains unaltered under various load conditions where the mechanical resonance of the jaw would be expected to vary greatly (Chapter 2). Such findings indicate a ‘neurogenic’ origin for this tremor. A possible candidate for this neurogenic component of physiological tremor in the jaw is the reflex feedback arising from the PMRs. Using local anaesthetisation, it has been shown in this thesis, that by blocking outflow from the PMRs, the amplitude of neurogenic physiological jaw tremor can be reduced dramatically. This procedure caused a dramatic reduction in not only the mechanical recordings of tremor but also in the coupling between masseteric muscles bilaterally (Chapter 3) and between single motor units recorded from within a homonymous muscle (Chapter 4). The obvious mechanism by which periodontal mechanoreceptor anaesthetisation could reduce the amplitude of physiological tremor in the jaw would be by reducing the amplitude of the oscillatory input to the motoneurones driving the tremor. This interpretation remains controversial however as physiological tremor in the jaw can be observed at force levels above which the PMRs are supposedly saturated in their response. In light of this knowledge, the saturating characteristics of these receptors in terms of reflex output were examined. To do this, a novel stimulation paradigm was devised whereby the incisal teeth were mechanically stimulated with identical stimulus waveforms superimposed upon increasing tooth preloads. This necessitated the use of a frequency response method to quantify the reflexes. An optimal frequency for stimulation was identified and used to confirm that the hyperbolic saturating response of PMRs observed previously, translated to a similar phenomenon in masticatory reflexes (Chapter 5). These data reinforced the idea that physiological tremor in the jaw was not just a consequence of rhythmic reflex input from PMRs, as the dynamic reflex response uncoupled from the input as the receptor-mediated reflex response saturated. An alternative hypothesis was then developed that suggested the effect of PMR suppression in physiological tremor was via tonic rather than rhythmic effects on the masseteric motoneurone pool. By utilising a novel contraction strategy to manipulate the mean firing rate of the motor neuron pool at a given level of force production, data contained in Chapter 6 shows that population motor unit firing statistics influence the expression of physiological tremor, and such manipulations mimic, to an extent, the changes in firing statistics and tremor amplitude seen during anaesthetisation of the PMRs. This thesis therefore posits a mechanism whereby periodontal input influences the firing rate of motoneurones in such a way as to promote tremulous activity (Chapter 5). However, as this proposed mechanism did not explain the full extent of tremor suppression seen during PMR anaesthetisation it can therefore only be considered a contributing factor in a multifactor process. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1297555 / Thesis (Ph.D.) -- University of Adelaide, School of Molecular and Biomedical Science, 2007