How to encourage employees to produce the innovative behavior in the business organization in this century are very important. This study hopes to discuss the relationship among the personal playfulness, Job involvenment, organization playfulness climate and employee's innovative behavior. It's taken 30 organizations and the effective volume was 1,213, the returns-ratio reached 95.21%. We will use HLM (hierarchical linear model) to analyze the data instead of the regression analysis in tradition. The major result of this study are as following: 1.Personal playfulness has positive effects on employee's innovative behavior. 2.Job involvenment has positive effects on employee's innovative behavior. 3.Organization playfulness climate has positive effects on employee's innovative behavior. 4.Organization playfulness climate has no moderate effect on the relationship between personal playfulness and employee's innovative behavior. 5.Organization playfulness climate has no moderate effect on the relationship between job involvenment and employee's innovative behavior.