Some yeast strains under certain conditions secrete polypeptide toxins which are inhibitory to sensitive fungal cells into the medium. These yeast strains are termed as killer yeasts and their toxins are designated as killer proteins or killer toxins. Killer proteins are classified into 11 typical types (K1-K11). These toxins have different killing mechanisms on sensitive cells. Some of them hydrolyze major cell wall component, beta-1,3- glucans. As mammalian cells lack cell walls research and development of novel highly selective antifungals are mostly focused on the agents which target the components of the fungal cell wall. K5 type killer protein was characterized in our labarotory previously. This protein is an exo beta-1,3-glucanase which is stable at pH&amp / #8217 / s and temperatures appropriate for its biocontrol usage. Beta-1,3-glucan hydrolyzing activity of the K5 type killer protein highlighted the potential use of this protein as a selective antifungal agent. According to CLSI methodology, antifungal activity of the K5 type yeast killer protein was tested against 6 fungal strains causing postharvest spoilage in citrus fruits and found to be effective on Botrytis cinerea, Penicillium digitatum, Penicillium italicum whereas non effective on Colletotrichum gloeosporoides, Phythophythora citrophthora, Alternaria citri. The MIC values of the toxin for B.cinerea, P.digitatum, P.italicum were found to be 16 mikrogram/ml while IC 50 values of the toxin were 2.12, 3.31, 2.57 mikrogram/ml respectively. The results showed that K5 type yeast killer protein would be used as a novel and selective agent against B.cinerea, P.digitatum and P.italicum.

Democracy was no more than a marginal force during the eighteenth century, unanimously denounced as a chimerical form of government unfit for passionate human beings living in commercial societies. Placed in this context this thesis studies the concept of ‘representative democracy' during the French Revolution, particularly under the Directory (1795–1799). At the time the term was an oxymoron. It was a neologism strategically coined by the democrats at a time when ‘representative government' and ‘democracy' were understood to be diametrically opposed to each other. In this thesis the democrats' political thought is simultaneously placed in several contexts. One is the rapidly changing political, economic and international circumstances of the French First Republic at war. Another is the anxiety about democratic decline emanating from the long-established intellectual traditions that regarded the history of Greece and Rome as proof that democracy and popular government inevitably led to anarchy, despotism and military government. Due to this anxiety the ruling republicans' answer during the Directory to the predicament—how to avoid the return of the Terror, win the war, and stabilize the Republic without inviting military government—was crystalized in the notion of ‘representative government', which defined a modern republic based on a firm rejection of ‘democratic' politics. Condorcet is important at this juncture because he directly challenged the given notions of his own period (such as that democracy inevitably fosters military government). Building on this context of debate, the arguments for democracy put forth by Antonelle, Chaussard, Français de Nantes and others are analysed. These democrats devised plans to steer France and Europe to what they regarded as the correct way of genuinely ending the Revolution: the democratic republic. The findings of this thesis elucidate the elements of continuity and those of rupture between the Enlightenment and the French Revolution.

If from God’s perspective ‘all the world’s a stage’, theology invites one to think and act according to the view afforded from this height. To speak theologically of a ‘world stage’ as many contemporary theologians have done has required rethinking the Church’s long-established antagonism towards the stage. Of late, theology has opened up academic exchange with the drama’s understanding of ‘the great theatre of the world’. Hans Urs von Balthasar’s theo-drama in particular has given Christians a means for entering into discussion with dramatic forms. Contemporary theological engagements with ‘drama’, however, have been limited to its most literary/metaphorical aspects; less attention has been paid to the potentialities in theology’s exchange with the performance aesthetics of live theatre. Pressed to its logical ends, however, von Balthasar’s idea of a ‘theological dramatics’ and its advances made in contemporary theology, suggest the need for sustained engagement with other modes of dramaturgy, including performance theory and the stage. This thesis attempts to instantiate this theological engagement through the aesthetics of theatrical performance.

This thesis is a study of royal acts printed in French before 1601. The kingdom of France is a natural place to begin a study of royal acts. It possessed one of the oldest judicial systems in Europe, which had been established during the reign of St Louis (1226-1270). By the sixteenth century, French kings were able to issue royal acts without any concern as to the distribution of their decrees. In addition, France was one of the leading printing centres in Europe. This research provides the first detailed analysis of this neglected category of texts, and examines the acts’ significance in French legal, political and printing culture. The analysis of royal acts reveals three key historical practices regarding the role of printing in judiciary matters and public affairs. The first is how the French crown communicated to the public. Chapters one and two discuss the royal process of dissemination of edicts and the language of royal acts. The second is how printers and publishers manoeuvred between the large number of royal promulgations and public demand. An overview of the printing industry of royal acts is provided in chapter three and the printers of these official documents are covered in chapter four. The study of royal acts also indicates which edicts were published frequently. The last two chapters examine the content of royal decrees and discuss the most reprinted acts. Chapter five explores the period before 1561 and the final chapter discusses the last forty years of the century. An appendix of all royal acts printed before 1601, which is the basis of my research for this study, is included. It is the first comprehensive catalogue of its kind and contains nearly six thousand entries of surviving royal acts printed before 1601.