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京都大学 / 0048 / 新制・課程博士 / 博士(農学) / 甲第17987号 / 農博第2034号 / 新制||農||1019(附属図書館) / 学位論文||H26||N4812(農学部図書室) / 80831 / 京都大学大学院農学研究科食品生物科学専攻 / (主査)教授 河田 照雄, 教授 谷 史人, 教授 保川 清 / 学位規則第4条第1項該当 / Doctor of Agricultural Science / Kyoto University / DFAM

A tolerância pode ser considerada um dos pilares da imunologia. Sabe-se que a tolerância a um antígeno pode gerar tolerância a outro antígeno não relacionado, fenômeno conhecido como tolerância cruzada. Neste trabalho caracterizamos a tolerância cruzada utilizando a OVA como tolerógeno e extrato de Blomia tropicalis (Bt) ou Hemocianina de Keyhole limpet (KLH) como alérgenos. Verificamos que é possível reproduzir o fenômeno da tolerância cruzada neste modelo de inflamação alérgica induzida tanto pelo KLH quanto pela Bt, com diminuição do infiltrado inflamatório no pulmão, eosinófilos, IgE total e produção de muco. Ainda, a estratégia de utilizar a tolerância cruzada terapeuticamente, i.é., após a sensibilização com KLH, a indução de tolerância cruzada não foi capaz de prevenir a resposta alérgica. Em conjunto, nossos dados mostram que a tolerância à OVA modifica as respostas alérgicas tanto à Bt quanto ao KLH no modelo de inflamação pulmonar experimental de forma profilática, mas que a tolerância cruzada não é eficiente em animais já sensibilizados. / Tolerance is among the Immunology pillars. Experimental data indicate that tolerance towards an antigen can promote tolerance to an unrelated antigen, a phenomenon known as cross-tolerance. Here we sought to characterize cross tolerance using OVA as a tolerogen and Blomia tropicalis (Bt) extract or Keyhole limpet Hemocianina (KLH) as allergens. We found that cross tolerance can be reproduced in the model of allergic lung disease induced by KLH or Bt, with less inflammatory infiltrate in the lung, eosinophils, total IgE and mucus production. Using cross tolerance therapeutically, i.e., after KLH sensitization, was not effective, since the allergic lung response was not modulated. Altogether, our data shows that OVA tolerance modulate allergic lung disease induced either by Bt or KLH when used as prophylactic model, however cross tolerance is ineffective in sensitized animals.

A tolerância pode ser considerada um dos pilares da imunologia. Sabe-se que a tolerância a um antígeno pode gerar tolerância a outro antígeno não relacionado, fenômeno conhecido como tolerância cruzada. Neste trabalho caracterizamos a tolerância cruzada utilizando a OVA como tolerógeno e extrato de Blomia tropicalis (Bt) ou Hemocianina de Keyhole limpet (KLH) como alérgenos. Verificamos que é possível reproduzir o fenômeno da tolerância cruzada neste modelo de inflamação alérgica induzida tanto pelo KLH quanto pela Bt, com diminuição do infiltrado inflamatório no pulmão, eosinófilos, IgE total e produção de muco. Ainda, a estratégia de utilizar a tolerância cruzada terapeuticamente, i.é., após a sensibilização com KLH, a indução de tolerância cruzada não foi capaz de prevenir a resposta alérgica. Em conjunto, nossos dados mostram que a tolerância à OVA modifica as respostas alérgicas tanto à Bt quanto ao KLH no modelo de inflamação pulmonar experimental de forma profilática, mas que a tolerância cruzada não é eficiente em animais já sensibilizados. / Tolerance is among the Immunology pillars. Experimental data indicate that tolerance towards an antigen can promote tolerance to an unrelated antigen, a phenomenon known as cross-tolerance. Here we sought to characterize cross tolerance using OVA as a tolerogen and Blomia tropicalis (Bt) extract or Keyhole limpet Hemocianina (KLH) as allergens. We found that cross tolerance can be reproduced in the model of allergic lung disease induced by KLH or Bt, with less inflammatory infiltrate in the lung, eosinophils, total IgE and mucus production. Using cross tolerance therapeutically, i.e., after KLH sensitization, was not effective, since the allergic lung response was not modulated. Altogether, our data shows that OVA tolerance modulate allergic lung disease induced either by Bt or KLH when used as prophylactic model, however cross tolerance is ineffective in sensitized animals.

The Master´s thesis addresses the design of wooden residential building. It is a four-storey building with a gabled roof with a slope of 15°. Building is designed for housing 32 people. The building is located on flat terrain. The main structural system are KLH solid wood panels with insulation layer on the outside. The ground floor is brick and consists of rooms of technical support and garages. The Master´s thesis includes project documentation for construction execution.

The aim of my master's thesis is a project of a new apartment building on a sloped site at a level of the documentation for the structure realization. The building is partly basement with four above-ground floor. Individual floors up recede and are roofed with flat roofs. The structural system of the basement part is made up of permanent shuttering and the above-ground part is made up wall of cross laminated timber construction panels KLH. The project of the building is in accordance with the development plan of Kopřivnice and with the current street built. The work puts great emphasis on layout including security of the construction in terms of statics, architecture, energy savings and safety at utilizing the structure.

Diploma thesis deals with construction technological project for extension of school. Contents of construction technological project are project engineering report, study of realization of main technological stages, engineering report of site equipment, proposal of construction machines, etc. In detail thesis deals with load-bearing structure of cross laminated timber and the technological note, inspection and testing plan.