The purpose is to identify the potential for the development of a creative economy in the territory of the Local Action Group Moravian Karst, located here especially due to close proximity to Brno and localization of unique natural and cultural heritage. Identifying potential is performed using the method of composite indicator, which links together disparate indicators of openness of the environment, advancement of the region and institutional backing. The result is the order of observed municipalities, ranked by degree of creative potential. Based on these findings the work proposed a set of recommendations Creativity 2021. The fulfillment of the steps indicated in the ideal case will lead to further work with creative potential and to strengthen the competitiveness of the selected region.

This Master thesis summarizes the characteristics of geological conditions in the Bohemian Karst and presents a proposal for organizing a geological excursion for lower-level gymnasium students or ninth-grade pupils to this territory. The goal of this Thesis is to set up a methodological proposal for preparing an excursion to the Bohemian Karst. It sums up the theoretical assumptions for organizing a geological excursion, lays down methodological proposals for on-site work and compiles worksheets. This Master thesis was created as a part project GA JU 065/2010/S

With a five-year survival rate of less than 5%, pancreatic cancer is one of the deadliest cancers. The most common activating mutations in pancreatic cancer are found in the KRAS gene, causing a constitutively-active KRAS protein in approximately 90% of pancreatic ductal adenocarcinomas (PDAC). PDAC-derived cell lines that harbor oncogenic KRAS mutations can be divided into two classes, KRAS dependent (or addicted) cells and KRAS independent cells. Oncogene dependency (or addiction) is a phenomenon where tu-mors require sustained activity of a single aberrantly activated gene despite the accumulation of multiple oncogenic lesions. In the case of PDAC, the single aberrantly activated gene is KRAS. KRAS independent cells have acquired various other oncogenic lesions that confer alternative cell survival signaling pathways to bypass oncogenic KRAS dependency. The Spleen Tyrosine Kinase (Syk) is highly expressed in KRAS dependent cells, while KRAS independent cells have low Syk expression. This pattern suggests that in KRAS dependent cells, constitutively active KRAS and Syk play a role in stimulating pro-survival pathways. One of these pro-survival pathways is known as mTORC1, which causes increased anabolic processes like protein and lipid synthesis. Accordingly, mTORC1 causes suppression of catabolic processes like autophagy. The net effect is in-creased cellular growth and proliferation. However, mTORC1 inhibitors have limited clinical efficacy, and potential therapeutic targets upstream of mTORC1 have drawn interest. Syk is a non-receptor tyrosine kinase that is an upstream activator of the mTORC1 pathway in hematopoietic malignancies. Through Syk inhibition studies using the small molecule PRT062607 (SYKi), we demonstrated that Syk is also involved in activating the mTORC1 pathway in KRAS dependent PDAC cells. However, the mechanism by which Syk-mediated activation of mTORC1 occurs is currently unknown. Moreover, it is unclear whether SYK kinase activity is required for the activation of the mTORC1 pathway. To address this issue, we introduced a single nucleotide mutation in the kinase do-main of Syk to render it kinase-inactive and found that Syk requires its kinase function to activate mTORC1. Studies using Syki also revealed that mTORC1 activity was also inhibited in KRAS independent PDAC cells that lack significant Syk expression. Interestingly, substrate specificity studies indicate that Syki also binds to and inhibits structurally similar protein tyrosine kinases such as the SRC Family Kinases (SFKs). Therefore, we designed an experiment to look for Syk and SFK cooperativity in regards to mTORC1 activation in PDAC cells. Our results indicate that the SFKs, Yes1 and Src display the most significant cooperative effect with Syk in activating the mTORC1 pathway. Src and Yes1 may even be involved in the upstream activation of Syk. To establish the physiological significance of Syk signaling in pancreatic cancer, it is important to establish model organisms that could be used for future studies. Thus, we test-ed Syk expression and function in PDAC cell lines derived from genetically-engineered mouse models (GEMM), which develop pancreatic cancer via oncogenic mutations in KRAS and TP53. We found that Syk is indeed expressed in murine PDAC cell lines and that the use of Syki in the murine PDAC cell lines results in decreased mTORC1 activity. These results recapitulate those obtained in human KRAS dependent PDAC cell lines. In summary, our studies show that Syk is a key regulator of mTORC1 signaling in human and mouse-derived pancreatic cancer cells. Syk kinase activity is required for mTORC1 activation. Finally, SFKs cooperate with Syk to promote robust mTORC1 activation. The mechanisms of SFK and Syk cooperativity in mTORC1 pathway activation will require further investigation. Additionally, our findings provide a strong rationale to study the effects of Syk kinase inhibition in physiologically-relevant murine models of pancreatic cancer. / 2021-06-08T00:00:00Z

The Diploma Thesis deals with the water pollution entering the Protected Landscape Area Moravian Karst via surface streams. In order to quantify the extent of the pollution surface streams entering the Moravian Karst were selected. A survey of a region of interest was done. Actual volume flow rate, measurements of physicochemical parameters, water samplings, laboratory analyses of samples were done monthly from April to November 2019. Conductivity, pH, temperature, oxidation-reduction potential, concentration of dissolved oxygen, and oxygen saturation were measured in situ. Afterwards, COD, BOD, phosphate, total phosphorus, ammonia, nitrites, nitrates and Kjeldahl nitrogen were determined in the laboratory. Organic nitrogen and total nitrogen were also calculated. The results were evaluated with a respect of Government Decree No. 401/2015 Coll. Material flows were calculated for selected parameters and used as other criterion that evaluate the water pollution of monitoring surface streams. Areas out of the PLA needing an enhanced protection of watercourses were proposed based on quantified pollution. Cooperation with two primary schools in the Moravian Karst supported the research. Pupils and their teachers were trained, and they measured phosphate in surface streams from April to November 2019. The website related to phosphorus in surface water were created and results of phosphate measurement were uploaded there.

The aim of this master thesis was to create a geographic information systém (GIS) for the needs of cycling. The proposed route connects Vyškov and Blansko and allows you to get to know beauties of the Moravian Karst and the Drahanská vrchovina. The whole thesis was created in software ArcGIS for Desktop 10.4.1 from the company Esri. In addition to the actual processing of data from measurement and GIS creation, I have tried various database queries and analytical task over the data. The final GIS was published on the web and is accessible to the public. It allows you to display the characteristics of the route and points of interest without having to turn off individual layers.

The aims of my diploma thesis are the design of a map of Drátenická cave surroundings in the Moravian Karst in the 1:500 scale with details utilizable by speleologists and the examination of a map of 1945 depicting the region of Drátenická cave. The thesis includes an elaboration of the theoretical foundations relevant for the fulfillment of the aims and the descriptions of the geodetic and processing procedures. The outcome of the thesis is the planimetric and altimetric survey, a new digital map of the region and the final examination of planimetric and altimetric components of the map of 1945.

Karst rivers are special type of rivers because of their hydrography conditions. They usually flow from non-karst area and as soon as they reach in to the karst area they disappear underground. There, they flow through cavities until they reach the surface again. The aim of this thesis is to assess the impact of water outflow from wastewater treatment plant on karst rivers. The study was done in the central area of CHKO Moravský kras where the impact of two WWTP on a cave system Rudické propadání – Býčí skála is evaluated. As a part of this study a half-year monitoring was carried out, which consisted of water sampling once every two weeks. In total, 13 water samples were taken from 8 sampling points. Also 4 additional one-time samples were taken from the cave system. Moreover, flow rate, temperature, conductivity, oxygen concentration and pH were measured at the sampling point. Afterwards, the content of undissolved solids, CODCr, BOD, N-NH4+, N-NO2-, N-NO3-, P-PO43- and total phosphorus were determined in the samples. The change of parameters in time and during the flow in the river was observed from the measured values. The balance of pollutants in particular parts of the river was calculated. The outflow values from waste water treatment plants were compared to the emission limits for surface waters and the parameters of rivers were compared to the environmental quality standards. According to the results, it can be observed that the water quality improves after passing through the cavities. It was proven that the outflows from the observed waste water treatment plants are negatively influencing the water streams. The most serious problem is the wash up of sludge from WWTP Rudice and its consequent sedimentation in the river and/or outflow to the Rudické propadání.

The aim of this thesis is to assess the impact of water outflow from wastewater treatment plant on karst rivers. The study was done in the northern area of Moravský kras protected landscape, where the impact of WWTP Ostrov u Macochy on a cave system Lopačské propadání was studied. As a part of this study a half-year monitoring was carried out, which consisted of water sampling every two weeks. In total, 13 water samples were taken from 7 sampling points. Moreover, flow rate, temperature, conductivity, oxygen concentration and pH were measured at the sampling point. Afterwards, the content of undissolved solids, COD, BOD, N-NH4+, N-NO2-, N-NO3-, NKi and total phosphorus were determined in the samples. The change of parameters in time on the sampling points and further along the flow of the river was evaluated from the measured values. The outflow values from waste water treatment plants were compared with the emission limits for surface waters and the values of river water parameters were compared with the environmental quality standards. It wasn’t proven that the outflow from the observed waste water treatment plant is negatively influencing the water stream. The most serious problems are: -The WWTP was designed for treatment of sewage from Ostrov u Macochy, which involved separate sanitary and storm sewers. After finishing the WWTP, a uniform sewer was built. -High concentration of total phosphorus on the outflow from WWTP. -Poor quality of Lopač brook water already before the outflow vent from WWTP.

In this thesis, the succession of spider assemblages in five limestone quarries of different ages in Bohemian Karst has been studied. The main goal of this paper was studying changes of spider assemblages along successional gradient. Furthermore, different environmental factors affecting spider communities and also assemblages of spider communites in different terraces of the quarry have been studied. Standardized methods (pitfall traps, hanging desk traps, shifting leaf litter, sweeping of herb vegetation, and beating shrubs) have been used for recording the highest possible richness of spiders. The results showed that position of the terraces and the age of the quarry do not affect species richness and density. Species density of epigeic spiders increased significantly with number of the plant species. The results showed that spiders do not have their own succession, but they are following vegetational succession. The environmental factors which affect species composition of spiders from the pitfall traps are positon of terraces, shading and moss cover. Although the age of the quarry was insignificant for spiders from pitfall traps, every quarry was inhabited by different spider communities. Shrubs and tree-dwelling spiders showed correlations with the age of the quarry. Data recorded from...

This thesis deals with evaluation of perception of forest paths for cycling, known as singletrack, located in the village called Jedovnice. In the first part, the concepts of terms such as tourism, cycletourism and singletrack are explained. The application of marketing tools is examined on the example of the project Singletrail Moravian Karst. The thesis also describes the implementation and results of the individual marketing tools. The results were processed on the basis of a questionnaire survey among visitors of forest trails of Singletrail Moravian Karst for comparison also among students and employees of Mendel University in Brno.