The objective of this study was to determine sprint athletes and non–athletes girls acute muscles and heart adaptation peculiarities to multiple anaerobic loads. The study‘s participants were girls of similar age: 5 trained athletes in sprint events and 5 non–athletes. There were estimated their antropometric parametres, assessed body mass index (BMI) and heart rate at rest. Also participants performed multiple anaerobic workloads. The results of this research showed that girls well–trained athletes managed to perform greater extent of physical load, were less tired and managed faster to recover between reiterations. The non–athletes performed significantly lower extent of physical load and before the third workload their heart rate‘s indices and results of running 10 m distance with 10 % of body mass weighting indicated considerable fatigue. In consideration of long term adaptation effect we propose, that well–trained athletes have lower body mass, BMI, heart rate at rest, higher running velocity and greater increase of velocity compare to non–athletes. Eaqual duration, intensity, rest pauses and the number of reiterations with and without weighting have different effect on muscle working ability. Athletes showed greater running velocity and it remained unaltered during reiterations in performing eaqual workload without weighting. Participants reached lower velocity and there were obtained velocity reduction in performance with weighting.