Le thon obèse (Thunnus obesus), le thon alabore (Thunnus albacares) et le listao (Katsuwonus pelamis) comptent parmi les espèces de thons les plus utilisées en conserve. Lors de la fabrication de conserves de thon, la substitution d'espèce et/ou le mélange de différentes espèces de thon sont interdits par la réglementation européenne. L'authentification des espèces de thon reste complexe à cause du degré de similitude élevé entre les espèces de thon, ou encore, lorsque les caractéristiques morphologiques externes sont éliminées au cours du filetage et lors de la mise en conserve. Par conséquent, des substitutions involontaires ou frauduleuses peuvent se produire. Dans cette étude, le marqueur mitochondrial du gène de la sous-unité 2 de la NADH déshydrogénase a été utilisé pour identifier le thon obèse et le gène de la sous-unité II de la cytochrome c oxydase a été utilisé pour identifier le thon albacore et le listao en utilisant la PCR en temps réel basée sur la technologie TaqMan. Deux méthodes différentes basées sur la qPCR ont été développées pour quantifier le pourcentage de chair de chaque espèce présente au sein d'une boîte de thon. La première a été basée sur la quantification absolue avec standard externe réalisée avec les deux marqueurs. La seconde a été basée sur la quantification relative avec standard externe avec le gène endogène de l'ARN 12S. Sur la base de ces résultats, nous pouvons conclure que notre méthode peut s'appliquer pour quantifier les deux espèces de thon albacore et obèse génétiquement très proches lorsqu'elles sont utilisées dans un mélange binaire en conserve. / Bigeye tuna (Thunnus obesus), yellowfin tuna (Thunnus albacares) and skipjack tuna (Katsuwonus pelanis) are among the most widely used tuna species for canning purposes. Not only substitution but also mixing of tuna species is prohibited by the European regulation for canned tuna products. However, it can be difficult to authenticate the tuna species, due to their high degree of similarity or even when the external morphological characteristics are removed due to filleting before canning. Consequently, involuntary or fraudulent substitutions may occur during the canning process. In this study, the mitochondrial marker from NADH dehydrogenase subunit 2 gene was used to identify bigeye tuna and the mitochondrial marker cytochrome c oxidase subunit II gene was used to identify yellowfin tuna and skipjack tuna, utilizing TaqMan qPCR methodology. Two different qPCR-based methods were developed to quantify the percentage of flesh of each species used for can processing. The first one was based on absolute quantification using standard curves realized with these two markers ; the second one was founded on relative quantification with the universal 12S rRNA gene as the endogenous gene. On the basis of our results, we conclude that our methodology could be applied to authenticate the two closely related tuna species (bigeye tuna and yellowfin tuna) when used in a binary mix in tuna cans.

Understanding the life history of a species is essential for fully understanding its role within an ecosystem. However, many of the fish species of high ecological value have not been studied due to their less prominent roles in local recreational and commercial fisheries in comparison to other targeted species. This study describes the age and growth patterns of three small tuna species inhabiting South Florida waters: blackfin tuna Thunnus atlanticus, little tunny Euthynnus alletteratus, and skipjack tuna Katsuwonus pelamis. Tuna specimens were collected via donations obtained from various fishing tournaments and charter captains in the areas of the Florida Straits as well as hook-and-line by the Nova Southeastern University Oceanographic Center. Age was described via sagittal otolith deposition patterns. They were removed, dried, sectioned, and rings were counted as well as measured. Validation of the timing of ring deposits was done by marginal increment analysis. Growth parameters were determined by comparison of fish fork length to count measurements. This comparison via the Von Bertalanffy growth equation produced a growth rate for each species: blackfin, L∞ = 95.34 cm, K = 0.28, and t0 = -1.53; little tunny, L∞ = 77.93 cm, K = 0.69, and t0 = -0.69; and skipjack, L∞ = 112.76 cm, K = 0.24, and t0 = -1.70. The curves indicate an average size of an individual of a given species at a certain age. They also give an estimation of a maximum length (L∞) of each species, in addition to specific growth rate, which is indicated by the slope. Parameters of each resulting Von Bertalanffy equation were compared among species. Results were also compared with growth rates currently used in stock assessments by fisheries management organizations, such as the International Commission for the Conservation of Atlantic Tunas (ICCAT).